Carboxyl radical induced cleavage of disulfide bonds in proteins. A .gamma.-ray and pulse radiolysis mechanistic investigation

Biochemistry ◽  
1990 ◽  
Vol 29 (49) ◽  
pp. 10978-10989 ◽  
Author(s):  
Vincent Favaudon ◽  
Herve Tourbez ◽  
Chantal Houee-Levin ◽  
Jean Marc Lhoste
Keyword(s):  
Disulfide Bonds ◽  
Pulse Radiolysis ◽  
Gamma Ray ◽  
Mechanistic Investigation ◽  
Carboxyl Radical

Pulse radiolysis of sodium formate aqueous solution up to 400 °C: Absorption spectra, kinetics and yield of carboxyl radical CO2−

2008 ◽  
Vol 77 (10-12) ◽  
pp. 1208-1212 ◽  
Author(s):  
Mingzhang Lin ◽  
Yosuke Katsumura ◽  
Yusa Muroya ◽  
Hui He ◽  
Toyoaki Miyazaki ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Absorption Spectra ◽  
Pulse Radiolysis ◽  
Sodium Formate ◽  
Carboxyl Radical

Mechanistic investigation of poly(ethylene imine)-based siRNA delivery: Disulfide bonds boost intracellular release of the cargo

2008 ◽  
Vol 130 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Miriam Breunig ◽  
Constantin Hozsa ◽  
Uta Lungwitz ◽  
Kazuo Watanabe ◽  
Isao Umeda ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Sirna Delivery ◽  
Ethylene Imine ◽  
Intracellular Release ◽  
Mechanistic Investigation ◽  
Poly Ethylene

On the possible gamma-ray source in Cygnus

1967 ◽  
Vol 31 ◽  
pp. 469-471
Author(s):  
J. G. Duthie ◽  
M. P. Savedoff ◽  
R. Cobb
Keyword(s):  
Gamma Rays ◽  
Gamma Ray ◽  
Right Ascension

A source of gamma rays has been found at right ascension 20h15m, declination +35°, with an uncertainty of 6° in each coordinate. Its flux is (1·5 ± 0·8) x 10-4photons cm-2sec-1at 100 MeV. Possible identifications are reviewed, but no conclusion is reached. The mechanism producing the radiation is also uncertain.

Solar Flare Energetic Neutral Emission Measurements in the Project Coronas-I

1994 ◽  
Vol 144 ◽  
pp. 635-639
Author(s):  
J. Baláž ◽  
A. V. Dmitriev ◽  
M. A. Kovalevskaya ◽  
K. Kudela ◽  
S. N. Kuznetsov ◽  
...  
Keyword(s):  
Solar Flare ◽  
Energy Range ◽  
Gamma Rays ◽  
Pulse Shape ◽  
Gamma Ray ◽  
Pulse Shape Discrimination ◽  
Shape Discrimination ◽  
Solar Neutron ◽  
Low Altitude

AbstractThe experiment SONG (SOlar Neutron and Gamma rays) for the low altitude satellite CORONAS-I is described. The instrument is capable to provide gamma-ray line and continuum detection in the energy range 0.1 – 100 MeV as well as detection of neutrons with energies above 30 MeV. As a by-product, the electrons in the range 11 – 108 MeV will be measured too. The pulse shape discrimination technique (PSD) is used.

Cryo-electron microscopy of two-dimensional crystals of reduced type B botulinum neurotoxin

1992 ◽  
Vol 50 (1) ◽  
pp. 530-531
Author(s):  
P. F. Flicker ◽  
V.S. Kulkarni ◽  
J. P. Robinson ◽  
G. Stubbs ◽  
B. R. DasGupta
Keyword(s):  
Disulfide Bonds ◽  
Clostridium Botulinum ◽  
Structural Changes ◽  
Two Dimensional ◽  
Type B ◽  
Single Chain ◽  
Muscle Paralysis ◽  
Cryo Electron Microscopy ◽  
Disulfide Linkages ◽  
Intracellular Action

Botulinum toxin is a potent neurotoxin produced by Clostridium botulinum. The toxin inhibits release of neurotransmitter, causing muscle paralysis. There are several serotypes, A to G, all of molecular weight about 150,000. The protein exists as a single chain or or as two chains, with two disulfide linkages. In a recent investigation on intracellular action of neurotoxins it was reported that type B neurotoxin can inhibit the release of Ca++-activated [3H] norepinephrine only if the disulfide bonds are reduced. In order to investigate possible structural changes in the toxin upon reduction of the disulfide bonds, we have prepared two-dimensional crystals of reduced type B neurotoxin. These two-dimensional crystals will be compared with those of the native (unreduced) type B toxin.

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