scholarly journals Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)] and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities

Biochemistry ◽  
1990 ◽  
Vol 29 (38) ◽  
pp. 9029-9039 ◽  
Author(s):  
Frank G. Loontiens ◽  
Peter Regenfuss ◽  
Annelies Zechel ◽  
Lieve Dumortier ◽  
Robert M. Clegg
Keyword(s):  
Wide Spectrum ◽  
Tight Binding ◽  
Calf Thymus Dna ◽  
Hoechst 33258 ◽  
Binding Characteristics ◽  
Calf Thymus

Thermodynamic Study of Rhodamine 123-Calf Thymus DNA Interaction: Determination of Calorimetric Enthalpy by Optical Melting Study

2014 ◽  
Vol 118 (46) ◽  
pp. 13151-13161 ◽  
Author(s):  
Abdulla Al Masum ◽  
Maharudra Chakraborty ◽  
Prateek Pandya ◽  
Umesh Chandra Halder ◽  
Md. Maidul Islam ◽  
...  
Keyword(s):  
Dna Interaction ◽  
Thermodynamic Study ◽  
Calf Thymus Dna ◽  
Rhodamine 123 ◽  
Calf Thymus

DUPLEX–TRIPLEX TRANSITION IN COMPLEXES OF NUCLEIC ACIDS WITH HOECHST 33258

2021 ◽  
Vol 55 (1 (254)) ◽  
pp. 39-45
Author(s):  
Marine A. Parsadanyan
Keyword(s):  
Ionic Strength ◽  
Nucleic Acids ◽  
Calf Thymus Dna ◽  
Hoechst 33258 ◽  
Groove Binding ◽  
Melting Curves ◽  
Calf Thymus ◽  
High Concentrations

The study of complexes of groove binding ligand Hoechst 33258 (H33258) with Calf Thymus DNA has been carried out. The data obtained revealed that the melting curves of the complexes of H33258 with DNA are monophasic at low ligand concentrations (0 < r ≤ 0.2) and become biphasic at relatively high concentrations (0.2 < r ≤ 0.33). This effect was revealed to depend on the ionic strength of the solution, and can also occur at high concentrations of the ligand. Comparison of the obtained data with the results for poly(rA)-poly(rU) and poly(dA)-poly(dT) shows a coincidence in the case of DNA and poly(rA)-poly(rU), while in the case of poly(dA)-poly(dT) the melting curves become biphasic at low ligand concentrations and actually do not depend on the ionic strength of the solution.

Fluorescence Interaction and Determination of Calf Thymus DNA with Two Ethidium Derivatives

2009 ◽  
Vol 19 (6) ◽  
pp. 1045-1051 ◽  
Author(s):  
Nuriye Akbay ◽  
Zeynel Seferoğlu ◽  
Elmas Gök
Keyword(s):  
Calf Thymus Dna ◽  
Calf Thymus

Binding Characteristics of Sodium Saccharin with Calf Thymus DNA in Vitro

2014 ◽  
Vol 62 (4) ◽  
pp. 991-1000 ◽  
Author(s):  
Guowen Zhang ◽  
Langhong Wang ◽  
Xiaoyue Zhou ◽  
Yu Li ◽  
Deming Gong
Keyword(s):  
Calf Thymus Dna ◽  
Binding Characteristics ◽  
Calf Thymus ◽  
Sodium Saccharin
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