Location of the carbohydrates present in the hydrogen ion-potassium ATPase vesicles isolated from hog gastric mucosa

Biochemistry ◽  
1990 ◽  
Vol 29 (3) ◽  
pp. 701-706 ◽  
Author(s):  
Kathleen Hall ◽  
Gonzalo Perez ◽  
Debra Anderson ◽  
Cecilia Gutierrez ◽  
Keith Munson ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Hydrogen Ion ◽  
Potassium Atpase

Relationship between potassium, acid secretion and bioelectric potentials of frog gastric mucosa in the presence of histamine and thiocyanate

1959 ◽  
Vol 196 (6) ◽  
pp. 1266-1269 ◽  
Author(s):  
John B. Harris ◽  
Isidore S. Edelman
Keyword(s):  
Gastric Mucosa ◽  
Acid Secretion ◽  
Potassium Concentration ◽  
Hydrogen Ion ◽  
Ion Secretion ◽  
Transient Rise ◽  
Net Flux ◽  
Bioelectric Potentials ◽  
Sustained Increase

The transmucosal potential difference (PD), the rate of H+ secretion and the net flux of potassium from nutrient to secretory phases (JnsK) of the frog gastric mucosa were studied in vitro by the chamber method. Histamine produced a fall in PD, a sustained increase in H+ production and an equivocal rise in JnsK. Increasing the nutrient potassium concentration (Kn) to 8.5 mEq/l. in the presence of histamine induced a depression in PD, although the rate of acid secretion was unchanged. Hydrogen ion secretion decreased when the nutrient potassium concentration was decreased to 1 mEq/l. despite the continued presence of histamine. The response of JnsK to alterations in nutrient potassium concentration was unaffected by the presence of either histamine or thiocyanate. Thiocyanate produced almost complete inhibition of H+ secretion and a rise in PD. Raising the nutrient potassium concentration in the presence of thiocyanate produced a prompt and sustained fall in PD, followed by a transient rise when Kn was lowered. Alterations of the nutrient potassium concentration in the presence of thiocyanate had no effect on the rate of acidification. The data indicate that under certain circumstances PD and H+ secretion can be uncoupled and that the inverse relationship between Kn and PD is substantially independent of the rate of H+ secretion.

Evidence for the presence of a carbohydrate moiety in fluorescein isothiocyanate labeled fragments of rat gastric hydrogen ion-potassium ATPase

Biochemistry ◽  
1989 ◽  
Vol 28 (8) ◽  
pp. 3183-3187 ◽  
Author(s):  
Mindy M. Tai ◽  
Wha Bin Im ◽  
John P. Davis ◽  
David P. Blakeman ◽  
Heidi A. Zurcher-Neely ◽  
...  
Keyword(s):  
Fluorescein Isothiocyanate ◽  
Carbohydrate Moiety ◽  
Hydrogen Ion ◽  
Potassium Atpase

Ionic fluxes of Rana pipiens stomach bathed by sulfate solutions

1964 ◽  
Vol 207 (6) ◽  
pp. 1173-1176 ◽  
Author(s):  
Sachiko Kaneko-Mohammed ◽  
C. Adrian M. Hogben
Keyword(s):  
Gastric Mucosa ◽  
Active Transport ◽  
Rana Pipiens ◽  
Sucrose Solution ◽  
Chloride Ion ◽  
Hydrogen Ion ◽  
Ion Pump ◽  
Ion Secretion ◽  
Sulfate Solutions ◽  
Ionic Fluxes

Efforts to understand the interdependence of the active transport of hydrogen and chloride ion by bathing gastric mucosae with sulfate-substituted salines have not led to agreement. For Rana pipiens, an independent hydrogen ion pump has been proposed, and an anion transporting system has been implicated in H+ transport by Rana catesbiana. This discrepancy prompted further study of the former gastric mucosa. The results show that the R. pipiens stomach does not actively transport SO4– and is able to secrete H+ without concomitant transport of anion. This is in agreement with the initial observation of Heinz and Durbin. Reversal of the spontaneous transepithelial potential upon exposure of the stomach to sulfate salines, however, is highly variable and in these experiments is attributed to a net transfer of Na+ from serosa to mucosa as well as to secretion of H+, not to the transport of the latter ion alone. For the gastric mucosa whose serosal aspect is bathed by sulfate saline, substitution of the mucosal fluid by an isoosmotic sucrose solution resulted in a reversible cessation of hydrogen ion secretion.

Relation between hydrogen ion secretion and oxygen uptake by gastric mucosa

1964 ◽  
Vol 206 (1) ◽  
pp. 218-222 ◽  
Author(s):  
J. G. Forte ◽  
R. E. Davies
Keyword(s):  
Gastric Mucosa ◽  
Acid Secretion ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Hydrogen Ion ◽  
Sodium Thiocyanate ◽  
Ph Stat ◽  
The Mean ◽  
Stat Method ◽  
Hydrogen Ion Transport

Bullfrog gastric mucosae were isolated, mounted between two glass chambers, and bathed with physiological salt solutions equilibrated with 5% CO2 and 95% O2. Oxygen consumption (qO2; measured polarographically) and acid secretion (qH+; pH stat method) were measured along with the transmucosal potential difference (p.d.) and current passing through the mucosa. Histamine (4 x 10–4 m) caused an increase in qH+ and qO2. In measurements on nine short-circuited mucosae the mean ratio for the ΔqH+/ΔqO2 was 2.1. Sodium thiocyanate (0.5–15 mm) caused a decrease in qH+ and qO2 and an increase in short-circuit current. These effects were reversible. The ratio of ΔqH+/ΔqO2 induced by thiocyanate varied from 5.0 to 12.0. Current (0.5 to 1.0 ma/cm2) passed through the mucosae, which reversed the normally observed p.d. to values between +70 and +240 mv (secretory side with respect to nutrient side in an external circuit), caused a decrease in qH+ and qO2; the average ΔqH+/ΔqO2 was approximately 13. Using either thiocyanate or electric current the ratio of the induced ΔqH+/ΔqO2 can really exceed 4.0, the electrochemical equivalent of oxygen, and thus if this extra oxygen provides the energy for the extra acid secretion these results invalidate a simple redox pump hypothesis of hydrogen ion transport by gastric mucosa.

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