Evidence for the presence of a carbohydrate moiety in fluorescein isothiocyanate labeled fragments of rat gastric hydrogen ion-potassium ATPase

Biochemistry ◽  
1989 ◽  
Vol 28 (8) ◽  
pp. 3183-3187 ◽  
Author(s):  
Mindy M. Tai ◽  
Wha Bin Im ◽  
John P. Davis ◽  
David P. Blakeman ◽  
Heidi A. Zurcher-Neely ◽  
...  
Keyword(s):  
Fluorescein Isothiocyanate ◽  
Carbohydrate Moiety ◽  
Hydrogen Ion ◽  
Potassium Atpase

Location of the carbohydrates present in the hydrogen ion-potassium ATPase vesicles isolated from hog gastric mucosa

Biochemistry ◽  
1990 ◽  
Vol 29 (3) ◽  
pp. 701-706 ◽  
Author(s):  
Kathleen Hall ◽  
Gonzalo Perez ◽  
Debra Anderson ◽  
Cecilia Gutierrez ◽  
Keith Munson ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Hydrogen Ion ◽  
Potassium Atpase

Physiological Effects of Nonimmune Platelet Associated Immunoglobulin G

1981 ◽  
Vol 45 (01) ◽  
pp. 027-033 ◽  
Author(s):  
K Sugiura ◽  
M Steiner ◽  
M Baldini
Keyword(s):  
Shape Change ◽  
Fluorescein Isothiocyanate ◽  
Recovery Phase ◽  
Human Platelets ◽  
Serotonin Release ◽  
Platelet Volume ◽  
Igg Antibody ◽  
Heterologous Antiserum ◽  
Igg Binding ◽  
Series Of Experiments

SummaryThe function of nonimmune IgG associated with platelets is unknown. In a series of experiments we have investigated this problem, relating amount of platelet-associated IgG (PAIgG) to platelet volume, serotonin release, adherence of platelets to monocytes and platelet senescence. Most of these studies were performed with human platelets. Platelets freed of preexisting PAIgG by incubation at 22° C were incubated with IgG in a series of concentrations ranging from 0.4 — 27.0 X10-6 M. The IgG preparations used were demonstrably free of aggregated forms of the protein. The amount of PAIgG bound to platelets was determined by the use of fluorescein isothiocyanate-conjugated anti-IgG antibody (F-anti-IgG antibody) which was quantified in a fluorospectrophotometer. Newly bound IgG was assayed similarly by the use of F-IgG. A dose-dependent increase in platelet volume was associated with the binding of nonimmune IgG by platelets. The process which leveled off at an IgG concentration of 1.2 —1.5 X10-5 M was almost fully reversible and was not due to platelet shape change or aggregation. Release of serotonin from IgG-treated platelets was relatively small but to the extent that it occurred was positively related to the IgG concentration to which platelets were exposed. Adherence to autologous monocytes studied quantitatively by the use of formaldehyde-fixed cells was also positively related to the amount of IgG on the platelets. Normal or IgG-defident serum had a potent inhibitory (noncompetitive) action on the binding of F-IgG and F-anti-human IgG antibody to human platelets. Cohorts of platelets prepared in rabbits during the recovery phase of immunological thrombocytopenia induced by injection of heterologous antiserum, showed an age-dependent increase of PAIgG and of IgG binding. These results suggest that PAIgG plays a role in the clearance of senescent platelets.

Interspecific variation of zona pellucida glycoconjugates in several species of marsupial

Reproduction ◽  
2000 ◽  
pp. 111-120 ◽  
Author(s):  
JA Chapman ◽  
OW Wiebkin ◽  
WG Breed
Keyword(s):  
Glycine Max ◽  
Sialic Acid ◽  
Zona Pellucida ◽  
Fluorescein Isothiocyanate ◽  
Interspecific Variation ◽  
Lectin Histochemistry ◽  
Sialic Acids ◽  
Arachis Hypogea ◽  
Marsupial Species ◽  
Similar Intensity

The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. Ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. Sections were also incubated with either neuraminidase or saponified, respectively, before incubation with the lectins to identify saccharide residues masked by sialic acids or O-acetyl groups on sialic acids. The zonae pellucidae surrounding the oocytes of the marsupials demonstrated interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Some of the zona pellucida glycoconjugates of all species, except those of the opossums, were masked by sialic acid with an increase in fluorescence with lectins from Arachis hypogea (PNA), and Glycine max (SBA), after desialylation. The disaccharide beta-galactose(1-4)N-acetyl-D-glucosamine appeared to be conformationally masked by O-acetyl groups of sialic acids in the zonae pellucidae of all species, with an increase in fluorescence with the lectin from Erythrina cristagalli (ECA), after saponification. Similar intensity and localization of beta-(1-4)-N-acetyl-D-glucosamine, as shown by staining of the lectin from Triticum vulgaris (WGA), to the inner and outer regions of the zona pellucida, were found to those reported in eutherian species. WGA fluorescence became uniform throughout the zonae pellucidae after saponification, indicating differential O-acetylation of sialic acids on the internal compartment of the zonae pellucidae.

Temporal Patterns of Acidification of Rivers in Nova Scotia and Newfoundland and their Relationship to Sulphate Emissions

1988 ◽  
Vol 23 (4) ◽  
pp. 532-541
Author(s):  
G. D. Howell ◽  
P.E.J. Green ◽  
C.A. Field ◽  
B. Freedman
Keyword(s):  
Statistical Analysis ◽  
Nova Scotia ◽  
Water Chemistry ◽  
Sulphur Dioxide ◽  
Low Ph ◽  
Hydrogen Ion ◽  
Atlantic Canada ◽  
Atmospheric Loading ◽  
Mineral Acids ◽  
Sulphur Dioxide Emission

Abstract Twenty-eight rivers in Atlantic Canada are sampled monthly, and have periods of record dating back as far as 1965. Statistical analysis of water chemistry for rivers in areas of Nova Scotia and Newfoundland that are believed to be sensitive to acidification has revealed that pH was significantly lower during the late 1960’s and early 1970’s. Annual hydrogen ion export for selected rivers was also greater during this period. This period of relatively low pH and large hydrogen ion export corresponds to a peak of North American sulphur dioxide emission, suggesting that these rivers may be responding to atmospheric loading of anthropogenic mineral acids.

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