Stimulation of the topoisomerase II induced DNA cleavage sites in the c-myc protooncogene by antitumor drugs is associated with gene expression

Biochemistry ◽  
1989 ◽  
Vol 28 (23) ◽  
pp. 9104-9110 ◽  
Author(s):  
Jean Francois Riou ◽  
Dominique Lefevre ◽  
Guy Riou
Keyword(s):  
Gene Expression ◽  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Antitumor Drugs ◽  
Cleavage Sites ◽  
Stimulation Of

scholarly journals In VitroEvolution of Preferred Topoisomerase II DNA Cleavage Sites

1999 ◽  
Vol 274 (8) ◽  
pp. 5227-5235 ◽  
Author(s):  
D. Andrew Burden ◽  
Neil Osheroff
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Cleavage Sites

In vivo stimulation by antitumor drugs of the topoisomerase II induced cleavage sites in c-myc protooncogene

1986 ◽  
Vol 137 (1) ◽  
pp. 154-160 ◽  
Author(s):  
Jean-François Riou ◽  
Eric Multon ◽  
Marie-José Vilarem ◽  
Christian-Jacques Larsen ◽  
Guy Riou
Keyword(s):  
Topoisomerase Ii ◽  
Antitumor Drugs ◽  
Cleavage Sites

Stimulation of topoisomerase II mediated DNA cleavage at specific sequence elements by the 2-nitroimidazole Ro 15-0216

Biochemistry ◽  
1990 ◽  
Vol 29 (41) ◽  
pp. 9507-9515 ◽  
Author(s):  
Boe S. Soerensen ◽  
Palle S. Jensen ◽  
Anni H. Andersen ◽  
Kent Christiansen ◽  
Jan Alsner ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Specific Sequence ◽  
Sequence Elements ◽  
Stimulation Of

scholarly journals Importance of the Fourth Alpha-Helix within the CAP Homology Domain of Type II Topoisomerase for DNA Cleavage Site Recognition and Quinolone Action

2002 ◽  
Vol 46 (9) ◽  
pp. 2735-2746 ◽  
Author(s):  
Dirk Strumberg ◽  
John L. Nitiss ◽  
Jiaowang Dong ◽  
Jerrylaine Walker ◽  
Marc C. Nicklaus ◽  
...  
Keyword(s):  
Amino Acid ◽  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Point Mutations ◽  
Alpha Helix ◽  
Single Amino Acid ◽  
Type Ii ◽  
Cleavage Sites ◽  
Amino Acid Residues ◽  
Site Recognition

ABSTRACT We report that point mutations causing alteration of the fourth alpha-helix (α4-helix) of the CAP homology domain of eukaryotic (Saccharomyces cerevisiae) type II topoisomerases (Ser740Trp, Gln743Pro, and Thr744Pro) change the selection of type II topoisomerase-mediated DNA cleavage sites promoted by Ca2+ or produced by etoposide, the fluoroquinolone CP-115,953, or mitoxantrone. By contrast, Thr744Ala substitution had minimal effect on Ca2+- and drug-stimulated DNA cleavage sites, indicating the selectivity of single amino acid substitutions within the α4-helix on type II topoisomerase-mediated DNA cleavage. The equivalent mutation in the gene for Escherichia coli gyrase causing Ser83Trp also changed the DNA cleavage pattern generated by Ca2+ or quinolones. Finally, Thr744Pro substitution in the yeast type II topoisomerase rendered the enzyme sensitive to antibacterial quinolones. This study shows that the α4-helix within the conserved CAP homology domain of type II topoisomerases is critical for selecting the sites of DNA cleavage. It also demonstrates that selective amino acid residues in the α4-helix are important in determining the activity and possibly the binding of quinolones to the topoisomerase II-DNA complexes.

Stimulation of Site-Specific Topoisomerase II-Mediated DNA Cleavage by an N-Methylpyrrolecarboxamide-anilinoacridine Conjugate: Relation to DNA Binding

Biochemistry ◽  
1994 ◽  
Vol 33 (33) ◽  
pp. 9865-9874 ◽  
Author(s):  
Philippe Fosse ◽  
Brigitte Rene ◽  
Jean-Marie Saucier ◽  
Jean-Pierre Henichart ◽  
Michael J. Waring ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Site Specific ◽  
Stimulation Of
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