Use of chemical modifications and site-directed mutagenesis to probe the functional role of thiol groups on the .gamma. subunit of Torpedo californica acetylcholine receptor

Laurent Pradier; Amy S. Yee; Mark G. McNamee

doi:10.1021/bi00442a006

Use of chemical modifications and site-directed mutagenesis to probe the functional role of thiol groups on the .gamma. subunit of Torpedo californica acetylcholine receptor

Biochemistry ◽

10.1021/bi00442a006 ◽

1989 ◽

Vol 28 (16) ◽

pp. 6562-6571 ◽

Cited By ~ 18

Author(s):

Laurent Pradier ◽

Amy S. Yee ◽

Mark G. McNamee

Keyword(s):

Acetylcholine Receptor ◽

Functional Role ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Thiol Groups ◽

Chemical Modifications ◽

Torpedo Californica ◽

Gamma Subunit

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Functional role of the cysteine 451 thiol group in the M4 helix of the .gamma. subunit of Torpedo californica acetylcholine receptor

Biochemistry ◽

10.1021/bi00475a003 ◽

1990 ◽

Vol 29 (23) ◽

pp. 5428-5436 ◽

Cited By ~ 30

Author(s):

Lian Li ◽

Mark Schuchard ◽

Andrew Palma ◽

Laurent Pradier ◽

Mark G. McNamee

Keyword(s):

Acetylcholine Receptor ◽

Functional Role ◽

Thiol Group ◽

Torpedo Californica ◽

Gamma Subunit

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Determination of the functional role of phenylalanine-31 of recombinant human dihydrofolate reductase by site-directed mutagenesis

Zurich, Switzerland, September 3–8, 1989 ◽

10.1515/9783110889000-148 ◽

1990 ◽

pp. 765-768

Keyword(s):

Dihydrofolate Reductase ◽

Functional Role ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Human Dihydrofolate Reductase

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Nitrate reductase of Neurospora crassa: the functional role of individual amino acids in the heme domain as examined by site-directed mutagenesis

MGG Molecular & General Genetics ◽

10.1007/bf00277060 ◽

1993 ◽

Vol 240 (2) ◽

pp. 221-230 ◽

Cited By ~ 11

Author(s):

Patricia M. Okamoto ◽

George A. Marzluf

Keyword(s):

Amino Acids ◽

Nitrate Reductase ◽

Neurospora Crassa ◽

Functional Role ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Heme Domain

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Role of a cysteine residue in substrate entry and catalysis in MtHIBADH : analysis by chemical modifications and site‐directed mutagenesis

IUBMB Life ◽

10.1002/iub.2466 ◽

2021 ◽

Author(s):

Amrita Singh ◽

Nagendar Goud Badepally ◽

Avadhesha Surolia

Keyword(s):

Cysteine Residue ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Chemical Modifications

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Evidence for the functional role of residues in the B′–C loop of baboon cytochrome P450 side-chain cleavage (CYP11A1) obtained by site-directed mutagenesis, kinetic analysis and homology modelling

The Journal of Steroid Biochemistry and Molecular Biology ◽

10.1016/j.jsbmb.2006.07.003 ◽

2007 ◽

Vol 103 (1) ◽

pp. 65-75 ◽

Cited By ~ 1

Author(s):

Karl-Heinz Storbeck ◽

Pieter Swart ◽

Sandra Graham ◽

Amanda C. Swart

Keyword(s):

Cytochrome P450 ◽

Kinetic Analysis ◽

Functional Role ◽

Homology Modelling ◽

Site Directed Mutagenesis ◽

Side Chain ◽

Directed Mutagenesis ◽

Side Chain Cleavage ◽

Chain Cleavage

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Characterization of the functional role of a flexible loop in the .alpha.-subunit of tryptophan synthase from Salmonella typhimurium by rapid-scanning, stopped-flow spectroscopy and site-directed mutagenesis

Biochemistry ◽

10.1021/bi00090a016 ◽

1993 ◽

Vol 32 (39) ◽

pp. 10404-10413 ◽

Cited By ~ 40

Author(s):

Peter S. Brzovic ◽

C. Craig Hyde ◽

Edith W. Miles ◽

Michael F. Dunn

Keyword(s):

Functional Role ◽

Site Directed Mutagenesis ◽

Alpha Subunit ◽

Directed Mutagenesis ◽

Stopped Flow ◽

Tryptophan Synthase ◽

Flexible Loop ◽

Rapid Scanning

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Investigation of the functional role of tryptophan-22 in Escherichia coli dihydrofolate reductase by site-directed mutagenesis

Biochemistry ◽

10.1021/bi00110a011 ◽

1991 ◽

Vol 30 (46) ◽

pp. 11092-11103 ◽

Cited By ~ 27

Author(s):

Mark S. Warren ◽

Katherine A. Brown ◽

Martin F. Farnum ◽

Elizabeth E. Howell ◽

Joseph Kraut

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Functional Role ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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The functional role of the binding site aspartate in muscarinic acetylcholine receptors, probed by site-directed mutagenesis

European Journal of Pharmacology Molecular Pharmacology ◽

10.1016/0922-4106(95)90151-5 ◽

1995 ◽

Vol 289 (3) ◽

pp. 429-437 ◽

Cited By ~ 39

Author(s):

Karen M. Page ◽

Carol A.M. Curtis ◽

Philip G. Jones ◽

Edward C. Hulme

Keyword(s):

Binding Site ◽

Acetylcholine Receptors ◽

Functional Role ◽

Muscarinic Acetylcholine Receptors ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Muscarinic Acetylcholine

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Probing the functional role of phenylalanine-31 of Escherichia coli dihydrofolate reductase by site-directed mutagenesis

Biochemistry ◽

10.1021/bi00387a053 ◽

1987 ◽

Vol 26 (13) ◽

pp. 4093-4100 ◽

Cited By ~ 45

Author(s):

Jin Tann Chen ◽

Kazunari Taira ◽

Chen Pei D. Tu ◽

Stephen J. Benkovic

Keyword(s):

Escherichia Coli ◽

Dihydrofolate Reductase ◽

Functional Role ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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The Functional Role of Positively Charged Amino Acid Side Chains in α-Bungarotoxin Revealed by Site-Directed Mutagenesis of a His-Tagged Recombinant α-Bungarotoxin†

Biochemistry ◽

10.1021/bi990045g ◽

1999 ◽

Vol 38 (24) ◽

pp. 7847-7855 ◽

Cited By ~ 29

Author(s):

Julie A. Rosenthal ◽

Mark M. Levandoski ◽

Belle Chang ◽

Jerald F. Potts ◽

Qing-Luo Shi ◽

...

Keyword(s):

Amino Acid ◽

Functional Role ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Side Chains ◽

Amino Acid Side Chains ◽

Positively Charged

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