Active-site modification of mammalian DNA polymerase .beta. with pyridoxal 5'-phosphate: mechanism of inhibition and identification of lysine 71 in the deoxynucleoside triphosphate binding pocket

Biochemistry ◽  
1989 ◽  
Vol 28 (15) ◽  
pp. 6305-6309 ◽  
Author(s):  
Amaresh Basu ◽  
Padmini Kedar ◽  
Samuel H. Wilson ◽  
Mukund J. Modak
Keyword(s):  
Dna Polymerase ◽  
Active Site ◽  
Binding Pocket ◽  
Dna Polymerase Beta ◽  
Polymerase Beta ◽  
Mechanism Of Inhibition ◽  
Deoxynucleoside Triphosphate

Structure-function analysis of the mammalian DNA polymerase .beta. active site: role of aspartic acid 256, arginine 254, and arginine 258 in nucleotidyl transfer

Biochemistry ◽  
1995 ◽  
Vol 34 (49) ◽  
pp. 15934-15942 ◽  
Author(s):  
Karen L. Menge ◽  
Zdenek Hostomsky ◽  
Beverly R. Nodes ◽  
Geoffrey O. Hudson ◽  
Soheil Rahmati ◽  
...  
Keyword(s):  
Structure Function ◽  
Aspartic Acid ◽  
Dna Polymerase ◽  
Active Site ◽  
Function Analysis ◽  
Dna Polymerase Beta ◽  
Polymerase Beta

scholarly journals Chick embryo DNA polymerase beta. Purified enzyme consists of a single Mr = 40,000 polypeptide.

1980 ◽  
Vol 255 (20) ◽  
pp. 9942-9948 ◽  
Author(s):  
M. Yamaguchi ◽  
K. Tanabe ◽  
Y.N. Taguchi ◽  
M. Nishizawa ◽  
T. Takahashi ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Dna Polymerase ◽  
Dna Polymerase Beta ◽  
Polymerase Beta

scholarly journals Studies of the domain structure of mammalian DNA polymerase beta. Identification of a discrete template binding domain.

1990 ◽  
Vol 265 (4) ◽  
pp. 2124-2131
Author(s):  
A Kumar ◽  
S G Widen ◽  
K R Williams ◽  
P Kedar ◽  
R L Karpel ◽  
...  
Keyword(s):  
Domain Structure ◽  
Dna Polymerase ◽  
Binding Domain ◽  
Dna Polymerase Beta ◽  
Polymerase Beta

scholarly journals Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis

1978 ◽  
Vol 173 (1) ◽  
pp. 309-314 ◽  
Author(s):  
T R Butt ◽  
W M Wood ◽  
E L McKay ◽  
R L P Adams
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Dna Polymerase Beta ◽  
Cell Nuclei ◽  
High Molecular Weight Dna ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta

The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.

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