Calcium-promoted DNA cleavage by eukaryotic topoisomerase II: trapping the covalent enzyme-DNA complex in an active form

Biochemistry ◽  
1987 ◽  
Vol 26 (14) ◽  
pp. 4303-4309 ◽  
Author(s):  
Neil Osheroff ◽  
E. Lynn Zechiedrich
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Active Form ◽  
Dna Complex

scholarly journals Structural domains of plant nuclear DNA as a constitutive component of the topoisomerase II/DNA complex.

1995 ◽  
Vol 42 (2) ◽  
pp. 201-204 ◽  
Author(s):  
V T Solovyan ◽  
I O Andreyev
Keyword(s):  
Dna Fragmentation ◽  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Nuclear Dna ◽  
Main Property ◽  
Reverse Reaction ◽  
Dna Fragments ◽  
Structural Domains ◽  
Chromatin Folding ◽  
Dna Complex

The treatment of agarose embedded plant nuclei by strong protein denaturants was demonstrated to result in discrete self-fragmentation of intact nuclear DNA. The set of resultant DNA cleavage products involves two main types of DNA fragments sized about 50-100 kb and 300-500 kb, being of the same type in various eukaryotic representatives. The pattern of ordered DNA fragmentation has been shown to be similar both in intact nuclei and in histone-depleted ones thus suggesting that the observed DNA fragments represent preexisting DNA structural domains, corresponding to the higher levels of chromatin folding. The topoisomerase II-specific poison teniposide (VM-26) has been shown to increase the ordered DNA cleavage while the conditions stimulating the topoisomerase II-mediated reverse reaction lead to the reassociation of the cleaved DNA domains. The data presented suggest that the nuclear DNA structural domains are involved in functioning of the topoisomerase II/DNA complex, the main property of which is its ability to mediate the cleavage/reassociation reactions.

scholarly journals Local base sequence preferences for DNA cleavage by mammalian topoisomerase II in the presence of amsacrine or teniposide

1991 ◽  
Vol 19 (24) ◽  
pp. 7003-7003 ◽  
Author(s):  
Y. Pommier ◽  
G. Capranico ◽  
A. Orr ◽  
K.W. Kohn
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Base Sequence ◽  
Local Base

scholarly journals Analysis of topoisomerase II-mediated DNA cleavage of the c-myc gene during HL60 differentiation

FEBS Letters ◽  
1993 ◽  
Vol 334 (3) ◽  
pp. 369-372 ◽  
Author(s):  
Jean-François Riou ◽  
Michèle Gabillot ◽  
Guy Riou
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Myc Gene

scholarly journals Drug Stimulated DNA Cleavage Mediated by Cauliflower Topoisomerase II

1991 ◽  
Vol 95 (2) ◽  
pp. 659-662 ◽  
Author(s):  
Boe S. Sørensen ◽  
Hideki Fukata ◽  
Palle S. Jensen ◽  
Anni H. Andersen ◽  
Kent Christiansen ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii

Altered catalytic activity of and DNA cleavage by DNA topoisomerase II from human leukemic cells selected for resistance to VM-26

Biochemistry ◽  
1988 ◽  
Vol 27 (24) ◽  
pp. 8861-8869 ◽  
Author(s):  
Mary K. Danks ◽  
Carla A. Schmidt ◽  
Margaret C. Cirtain ◽  
D. Parker Suttle ◽  
William T. Beck
Keyword(s):  
Catalytic Activity ◽  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Leukemic Cells ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Human Leukemic Cells

Topoisomerase-II-Mediated DNA Cleavage within the Human Ribosomal Genes

1995 ◽  
Vol 213 (1) ◽  
pp. 282-288 ◽  
Author(s):  
M. Govoni ◽  
S. Neri ◽  
T. Labella ◽  
J.E. Sylvester ◽  
F. Novello ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Ribosomal Genes

Glycosylated flavones as selective inhibitors of topoisomerase IV.

1997 ◽  
Vol 41 (5) ◽  
pp. 992-998 ◽  
Author(s):  
F X Bernard ◽  
S Sablé ◽  
B Cameron ◽  
J Provost ◽  
J F Desnottes ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Coli Strain ◽  
Selective Inhibitors ◽  
Topoisomerase Iv ◽  
Gene Encoding ◽  
Cottonseed Flour ◽  
Dna Topoisomerase ◽  
E Coli ◽  
Pbr322 Dna

Three flavonoids which promoted Escherichia coli topoisomerase IV-dependent DNA cleavage were isolated from cottonseed flour and identified as quercetin 3-O-beta-D-glucose-[1,6]-O-alpha-L-rhamnose (rutin), quercetin 3-O-beta-D-galactose-[1,6]-O-alpha-L-rhamnose, and quercetin 3-O-beta-D-glucose (isoquercitrin). The most active one (rutin) also inhibited topoisomerase IV-dependent decatenation activity (50% inhibitory concentration, 64 microg/ml) and induced the SOS response of a permeable E. coli strain. Derivatives of quercetin glycosylated at position C-3 were shown to induce two site-specific DNA cleavages of pBR322 DNA, which were mapped by DNA sequence analysis to the gene encoding resistance to tetracycline. Cleavage at these sites was hardly detectable in cleavage reactions with quercetin or fluoroquinolones. None of the three flavonoids isolated from cottonseeds had any stimulatory activity on E. coli DNA gyrase-dependent or calf thymus topoisomerase II-dependent DNA cleavage, and they were therefore specific to topoisomerase IV. These results show that selective inhibitors of topoisomerase IV can be derived from the flavone structure. This is the first report on a DNA topoisomerase inhibitor specific for topoisomerase IV.

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