Transverse location of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene in model lipid bilayer membrane systems by resonance excitation energy transfer

Biochemistry ◽  
1985 ◽  
Vol 24 (15) ◽  
pp. 4097-4108 ◽  
Author(s):  
Lesley Davenport ◽  
Robert E. Dale ◽  
Roger H. Bisby ◽  
Robert B. Cundall
1977 ◽  
Vol 32 (2) ◽  
pp. 140-143 ◽  
Author(s):  
J. Kamiński ◽  
A. Kawski

In studying the radiationless energy transfer between unlike molecules (heterotransfer) in fluid and rigid solutions the fluctuations of the concentration of the acceptor molecules, as well as the dependence of the probability of resonance excitation energy transfer on the mutual orientation of the transition moments of the interacting donor and acceptor molecules have been taken into account. With these and the assumptions of the shell model of a luminescent centre (A. Kawski and J. Kaminski, Z. Naturforsch. 29 a, 452 [1974]) one obtains the Förster expression for the quantum yield of the donor fluorescence quenched by foreign absorbing substances


2014 ◽  
Vol 11 (95) ◽  
pp. 20140069 ◽  
Author(s):  
Ben Kent ◽  
Taavi Hunt ◽  
Tamim A. Darwish ◽  
Thomas Hauß ◽  
Christopher J. Garvey ◽  
...  

Trehalose, a natural disaccharide with bioprotective properties, is widely recognized for its ability to preserve biological membranes during freezing and dehydration events. Despite debate over the molecular mechanisms by which this is achieved, and that different mechanisms imply quite different distributions of trehalose molecules with respect to the bilayer, there are no direct experimental data describing the location of trehalose within lipid bilayer membrane systems during dehydration. Here, we use neutron membrane diffraction to conclusively show that the trehalose distribution in a dioleoylphosphatidylcholine (DOPC) system follows a Gaussian profile centred in the water layer between bilayers. The absence of any preference for localizing near the lipid headgroups of the bilayers indicates that the bioprotective effects of trehalose at physiologically relevant concentrations are the result of non-specific mechanisms that do not rely on direct interactions with the lipid headgroups.


ACS Nano ◽  
2015 ◽  
Vol 9 (2) ◽  
pp. 1552-1560 ◽  
Author(s):  
Yan Qiao ◽  
Frank Polzer ◽  
Holm Kirmse ◽  
Egon Steeg ◽  
Sergei Kühn ◽  
...  

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