Chloroplast biogenesis 89: development of analytical tools for probing the biosynthetic topography of photosynthetic membranes by determination of resonance excitation energy transfer distances separating metabolic tetrapyrrole donors from chlorophyll a acceptors

2004 ◽  
Vol 329 (2) ◽  
pp. 207-219 ◽  
Author(s):  
Karen J. Kopetz ◽  
Vladimir L. Kolossov ◽  
Constantin A. Rebeiz
Keyword(s):  
Energy Transfer ◽  
Excitation Energy ◽  
Chlorophyll A ◽  
Excitation Energy Transfer ◽  
Resonance Excitation ◽  
Chloroplast Biogenesis ◽  
Photosynthetic Membranes ◽  
Analytical Tools

Elektronenanregungsenergieiibergang zwischen ungleichartigen Molekiilen in Losung / Electronic Excitation Energy Transition among unlike Molecules in Solution

1977 ◽  
Vol 32 (2) ◽  
pp. 140-143 ◽  
Author(s):  
J. Kamiński ◽  
A. Kawski
Keyword(s):  
Energy Transfer ◽  
Excitation Energy ◽  
Excitation Energy Transfer ◽  
Energy Transition ◽  
Mutual Orientation ◽  
Resonance Excitation ◽  
Electronic Excitation Energy ◽  
Radiationless Energy Transfer ◽  
Donor And Acceptor ◽  
Acceptor Molecules

In studying the radiationless energy transfer between unlike molecules (heterotransfer) in fluid and rigid solutions the fluctuations of the concentration of the acceptor molecules, as well as the dependence of the probability of resonance excitation energy transfer on the mutual orientation of the transition moments of the interacting donor and acceptor molecules have been taken into account. With these and the assumptions of the shell model of a luminescent centre (A. Kawski and J. Kaminski, Z. Naturforsch. 29 a, 452 [1974]) one obtains the Förster expression for the quantum yield of the donor fluorescence quenched by foreign absorbing substances

Excitation Energy Transfer among Chlorophyll a Molecules in Polystyrene: Concentration Dependence of Quantum Yield, Polarization and Lifetime of Fluorescence

1978 ◽  
Vol 33 (11-12) ◽  
pp. 863-869 ◽  
Author(s):  
D. Wong ◽  
Govindjee ◽  
H. Merkelo ◽  
K. Vacek
Keyword(s):  
Energy Transfer ◽  
Quantum Yield ◽  
Excitation Energy ◽  
Chlorophyll A ◽  
Excitation Energy Transfer ◽  
Concentration Quenching ◽  
Electronic Excitation Energy Transfer ◽  
Electronic Excitation Energy ◽  
Polarization Of Fluorescence ◽  
Total Concentrations

Electronic excitation energy transfer was studied for chlorophyll a in a solid solution of poly­styrene by measuring the concentration quenching of quantum yield, polarization, and lifetime of fluorescence. The concentration quenching of the experimental fluorescence quantum yield is adequately described by Kelly and Porter’s empirical formula (Proc. Roy. Soc., Lond. A 315, 149, 1970), and of polarization of fluorescence by the Jablonski theory (Acta Phys. Pol., 14, 295, 1955). With increasing concentration of chlorophyll a, the fluorescence peak at 672 nm (mainly monomer) is red-shifted, the intensity of the emission peak at ∼730 nm (mainly aggregate) relative to that at the shorter wavelength is increased. The R̅0 values, calculated by using total concentrations, for the emission at 672 nm and 730 nm are 73 ± 2 Å and 45 ±1 Å, respectively. This may suggest that the chlorophyll monomers have a greater efficiency of energy transfer than the aggregates, which fluoresce at ∼ 730 nm.

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