Investigation of structure and rate of synthesis of ornithine decarboxylase protein in mouse kidney

Biochemistry ◽  
1984 ◽  
Vol 23 (16) ◽  
pp. 3777-3783 ◽  
Author(s):  
Lo Persson ◽  
James E. Seely ◽  
Anthony E. Pegg
Keyword(s):  
Ornithine Decarboxylase ◽  
Mouse Kidney

Heterogenous expression of ornithine decarboxylase gene in the proximal tubule of the mouse kidney following testosterone treatment

1993 ◽  
Vol 100 (5) ◽  
pp. 325-330 ◽  
Author(s):  
Noriyuki Koibuchi ◽  
Shigeru Matsuzaki ◽  
Mikako Sakai ◽  
Hideki Ohtake ◽  
Sadao Yamaoka
Keyword(s):  
Proximal Tubule ◽  
Ornithine Decarboxylase ◽  
Mouse Kidney ◽  
Testosterone Treatment

scholarly journals Studies of mammalian ornithine decarboxylase using a monoclonal antibody

1984 ◽  
Vol 217 (1) ◽  
pp. 123-128 ◽  
Author(s):  
A E Pegg ◽  
J E Seely ◽  
L Persson ◽  
M Herlyn ◽  
K Ponsell ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ornithine Decarboxylase ◽  
Mouse Kidney ◽  
Immunoglobulin M ◽  
Active Enzyme ◽  
Sodium Thiocyanate ◽  
Immunoaffinity Column ◽  
Rapid Turnover ◽  
Cytosolic Protein

A monoclonal antibody of the immunoglobulin M class was produced against mouse kidney ornithine decarboxylase. Screening for the antibody was carried out using alpha-difluoromethyl[5-3H]ornithine-labelled ornithine decarboxylase. The antibody reacted with this antigen and with native ornithine decarboxylase. The antibody attached to Sepharose could be used to form an immunoaffinity column that retained mammalian ornithine decarboxylase. The active enzyme could then be eluted in a highly purified form by 1.0M-sodium thiocyanate. The monoclonal antibody could also be used to precipitate labelled ornithine decarboxylase from homogenates of kidneys from androgen-treated mice given [35S]methionine. Only one band, corresponding to Mr of about 55000, was observed. The extensive labelling of this band is consistent with the rapid turnover of ornithine decarboxylase protein, since this enzyme represents only about 1 part in 10000 of the cytosolic protein.

scholarly journals Ornithine decarboxylase antizyme in kidneys of male and female mice

1988 ◽  
Vol 254 (2) ◽  
pp. 367-372 ◽  
Author(s):  
Y Murakami ◽  
M Marumo ◽  
S I Hayashi
Keyword(s):  
Ornithine Decarboxylase ◽  
Half Life ◽  
Mouse Kidney ◽  
Repeated Injection ◽  
Male Mice ◽  
Protein Inhibitor ◽  
Male And Female ◽  
Female Mice ◽  
Htc Cells

Antizyme, a protein inhibitor of ornithine decarboxylase (ODC), was shown to be induced in mouse kidney by repeated injection of putrescine. Antizyme was also present as a complex with ODC in the kidney of untreated mouse. The amount of the renal ODC-antizyme complex was 3-fold higher in male mice than in female mice. On the contrary, the proportion of ODC present as a complex with antizyme was 24-fold higher in females than in males, and the decay of renal ODC activity after cycloheximide treatment was about 5-fold more rapid in females than in males. Administration of testosterone to female mice, a procedure known to prolong the half-life of renal ODC, increased both ODC activity and the content of ODC-antizyme complex, but decreased the antizyme/ODC ratio in the kidney. These results are consistent with the previous observation in HTC cells that the decay rate of ODC activity in the presence of cycloheximide correlated well with the proportion of ODC present as a complex with antizyme, suggesting the ubiquitous role of antizyme in ODC degradation.

scholarly journals Inhibition of Ornithine Decarboxylase Activity in Mouse Kidney by Administration of Diamines.

1979 ◽  
Vol 33b ◽  
pp. 537-540 ◽  
Author(s):  
Lo Persson ◽  
Elsa Rosengren ◽  
Siv Osterman-Golkar ◽  
Dan Segerbäck ◽  
Curt R. Enzell ◽  
...  
Keyword(s):  
Ornithine Decarboxylase ◽  
Mouse Kidney ◽  
Decarboxylase Activity ◽  
Ornithine Decarboxylase Activity

Induction of ornithine decarboxylase immunoreactivity in the male mouse kidney following testosterone treatment: an axial heterogeneity in the proximal tubule

1993 ◽  
Vol 136 (1) ◽  
pp. 85-NP ◽  
Author(s):  
N. Koibuchi ◽  
S. Matsuzaki ◽  
H.-T. Ma ◽  
M. Sakai ◽  
S. Yamaoka
Keyword(s):  
Proximal Tubule ◽  
Ornithine Decarboxylase ◽  
Protein Level ◽  
Male Mouse ◽  
Subcutaneous Injection ◽  
Mouse Kidney ◽  
Luminal Surface ◽  
Testosterone Treatment ◽  
Pars Recta ◽  
Pars Convoluta

ABSTRACT The effect of testosterone on the activity of ornithine decarboxylase (ODC), its protein level and immunocytochemical distribution were examined in the mouse kidney. Male BALB C mice at 8 weeks of age were used throughout. Fourteen hours before death, they received a subcutaneous injection of testosterone (1 mg/animal) or solvent to measure renal ODC activity or to detect the distribution of ODC immunoreactivity in the kidney. Renal ODC activity and the content of the enzyme were markedly increased after testosterone treatment. Histologically, few cells that were obviously immunoreactive to ODC were observed in the control animals and in the testosterone-treated animals a marked increase in ODC immunoreactivity was observed only in the cortex. ODC immunoreactive cells were located diffusely in the proximal tubule. In the pars recta, cells were stained weakly and homogeneously, while in the pars convoluta, the luminal surface of the cells showed stronger immunoreactivity. Moreover, many granule-like particles that were strongly ODC immunoreactive were observed inside the lumen of the pars convoluta. These results show that testosterone treatment induces an increase in ODC content in certain cells located in the proximal tubule of the cortex. Journal of Endocrinology (1993) 136, 85–89

scholarly journals Androgen induction of ornithine decarboxylase mRNA in mouse kidney as studied by complementary DNA.

1984 ◽  
Vol 81 (3) ◽  
pp. 731-735 ◽  
Author(s):  
K. K. Kontula ◽  
T. K. Torkkeli ◽  
C. W. Bardin ◽  
O. A. Janne
Keyword(s):  
Ornithine Decarboxylase ◽  
Mouse Kidney ◽  
Complementary Dna
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