Epidermal filaggrin is synthesized on a large messenger ribonucleic acid as a high-molecular-weight precursor

Biochemistry ◽  
1983 ◽  
Vol 22 (21) ◽  
pp. 4867-4871 ◽  
Author(s):  
Rick L. Meek ◽  
John D. Lonsdale-Eccles ◽  
Beverly A. Dale
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid

scholarly journals High-molecular-weight nuclear polyadenylate-containing ribonucleic acid isolated from the lactating guniea-pig mammary gland contains milk-protein messenger ribonucleic acid sequences

1979 ◽  
Vol 181 (2) ◽  
pp. 501-504 ◽  
Author(s):  
I C Bathurst ◽  
R K Craig ◽  
P N Campbell
Keyword(s):  
Molecular Weight ◽  
Mammary Gland ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Milk Protein ◽  
Gradient Centrifugation ◽  
Nucleic Acid Hybridization ◽  
Nuclear Fraction ◽  
Messenger Ribonucleic Acid ◽  
Precursor Rna

1. Steady-state poly(A)-containing nuclear RNA was isolated from the lactating guinea-pig mammary gland and analysed by sucrose-gradient centrifugation under denaturing conditions. 2. Nucleic acid-hybridization studies demonstrated the presence of small amounts of high-molecular-weight RNA species containing milk-protein mRNA sequences sedimenting at 25S. These were not found in the post-nuclear supernatant, where milk-protein sequences sedimented only between 12S and 15S; these were also the predominant species in the nuclear fraction. 3. The results are consistent with the transcription of milk-protein genes as high-molecular-weight precursor RNA species, 3-4 times as large as the active mRNA species isolated from the post-nuclear fraction.

scholarly journals Terminal sequence studies of high-molecular-weight ribonucleic acid. The 3′-termini of rabbit globin messenger ribonucleic acid

1973 ◽  
Vol 131 (2) ◽  
pp. 315-325 ◽  
Author(s):  
J. A. Hunt
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Periodate Oxidation ◽  
Apparent Molecular Weight ◽  
Paper Electrophoresis ◽  
Terminal Sequence ◽  
Globin Mrna ◽  
Messenger Ribonucleic Acid ◽  
Oxidation Analysis

Haemoglobin mRNA isolated from EDTA-treated polyribosomes has an apparent molecular weight of 120000–180000 estimated by condensation with 3H-labelled isoniazid after periodate oxidation. Analysis of the ribonuclease digests of isoniazid-labelled RNA by paper electrophoresis and column chromatography enables the amount of contaminating 18S, 7S, 5S and 4S RNA to be estimated, and a corrected molecular weight of globin mRNA as the acid is 161000 or 500 nucleotides in length. This molecule contains two groups of 3′-terminal sequences in equal yield; G-Y-A6 and G-Y-A7 in the ratio 3:2, and G-N9–16-Y-A2 and G-N9–16-Y-N3 in the ratio 3:2. The significance of these sequences is discussed in relation to the poly(A) content of globin mRNA, the specificity of the sequences, and possible function in processing and biosynthesis of mRNA.

scholarly journals The detection of messenger ribonucleic acid sequences in heterogeneous nuclear ribonucleic acid fractions of the oestrogen-stimulated rat uterus

1980 ◽  
Vol 187 (1) ◽  
pp. 265-267 ◽  
Author(s):  
S Aziz ◽  
J T Knowler
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Mrna Sequence ◽  
Rat Uterus ◽  
Complementary Dna ◽  
Messenger Ribonucleic Acid ◽  
Complementary Sequences ◽  
Nuclear Rna

cDNA (complementary DNA) complementary to the abundant sequences of mRNA isolated from oestrogen-stimulated uterus was hybridized to polyadenylated and non-polyadenylated uterine hnRNA (heterogeneous nuclear RNA). High-molecular-weight polyadenylated hnRNA, isolated under denaturing conditions, was able to saturate the cDNA, but complementary sequences were low abundance when compared with homologous mRNA. The mRNA sequence content of the polyadenylated hnRNA increased considerably during oestrogen-induced growth of the uterus. Non-polyadenylated hnRNA also contained sequences complementary to the cDNA.

scholarly journals The Structure of High Molecular Weight Ribonucleic Acid in Solution

1961 ◽  
Vol 1 (7) ◽  
pp. 525-537 ◽  
Author(s):  
Serge N. Timasheff ◽  
J. Witz ◽  
V. Luzzati
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid

scholarly journals The presence of a high-molecular-weight (guanine-plus-cytosine)-rich segment at the 3' end of rabbit 28S ribosomal ribonucleic acid.

1975 ◽  
Vol 147 (3) ◽  
pp. 625-628 ◽  
Author(s):  
A A Hadjiolov ◽  
R A Cox ◽  
P Huvos
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Average Molecular Weight ◽  
28S Rrna ◽  
Number Average Molecular Weight ◽  
Ribonuclease T1 ◽  
Naphthoic Acid ◽  
Rrna Molecule ◽  
Ribosomal Ribonucleic Acid

The 3′ hydroxyl end of 28S L-rRNA (major RNA species of the larger subribosomal particle) was labelled by coupling its 2-hydroxy-3-naphthoic acid hydrazine with diazotized [3H]aniline. The RNA was hydrolysed partially with ribonuclease T1 and fractionated on Sephadex G-200. The results show that a highly structured segment with 78% G+C content and a number-average molecular weight of at least 1.0×10(5)-1.8×10(5) is located at the 3′ hydroxyl end of the 28S rRNA molecule.

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