Structural studies on the membrane-bound immunoglobulin E-receptor complex. 1. Characterization of large plasma membrane vesicles from rat basophilic leukemia cells and insertion of amphipathic fluorescent probes

Biochemistry ◽  
1983 ◽  
Vol 22 (14) ◽  
pp. 3466-3474 ◽  
Author(s):  
David Holowka ◽  
Barbara Baird
Keyword(s):  
Immunoglobulin E ◽  
Membrane Vesicles ◽  
Leukemia Cells ◽  
Receptor Complex ◽  
Structural Studies ◽  
Plasma Membrane Vesicles ◽  
Rat Basophilic Leukemia Cells ◽  
Membrane Bound ◽  
Basophilic Leukemia

Immunoglobulin E mediated membrane conductance changes in rat basophilic leukemia cells

1988 ◽  
Vol 66 (3) ◽  
pp. 328-331 ◽  
Author(s):  
Carlos Barajas-López ◽  
Jan D. Huizinga
Keyword(s):  
Immunoglobulin E ◽  
Calcium Influx ◽  
Membrane Conductance ◽  
Membrane Resistance ◽  
Leukemia Cells ◽  
Rat Basophilic Leukemia Cells ◽  
Conductance Changes ◽  
Electrophysiological Effects ◽  
Basophilic Leukemia ◽  
Stimulation Of

Electrophysiological effects of anaphylactic stimulation of rat basophilic leukemia cells (RBL-2H3) were studied using conventional microelectrodes. Stimulation of passively sensitized cells by anti-immunoglobulin E resulted in hyperpolarization followed by depolarization. These changes in membrane polarization were associated with a decrease in input membrane resistance. No effect of anaphylactic stimulation was seen in Ca2+-free solution or when Ca2+ influx was blocked by Co2+, but it was mimicked by the Ca2+ ionophore A-23187. This suggests that the changes in ionic conductances were associated with calcium influx. These results support the hypothesis that membrane conductance changes are involved in the stimulus-secretion process of the RBL-2H3 cells.

scholarly journals Small oligomers of immunoglobulin E (IgE) cause large-scale clustering of IgE receptors on the surface of rat basophilic leukemia cells.

1984 ◽  
Vol 98 (2) ◽  
pp. 577-583 ◽  
Author(s):  
A K Menon ◽  
D Holowka ◽  
B Baird
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Surface ◽  
Large Scale ◽  
Immunoglobulin E ◽  
Leukemia Cells ◽  
Ige Receptors ◽  
Rat Basophilic Leukemia Cells ◽  
A Cell ◽  
Basophilic Leukemia

We examined the distribution of small oligomers of IgE bound to rat basophilic leukemia cells using fluorescence microscopy. The oligomers were seen to cluster into visible patches on the cell surface at 4 degrees C; at higher temperatures internalization also was observed. In contrast, cells labeled with IgE monomers remained predominantly ring-stained. Evidence is provided that the observed clustering of IgE oligomers is a cell-induced phenomenon, and the possible significance of this clustering is discussed in the context of the oligomer-triggered degranulation of rat basophilic leukemia cells.

Characterization of the receptors for IgE on membranes isolated from rat basophilic leukemia cells

1982 ◽  
Vol 19 (7) ◽  
pp. 925-941 ◽  
Author(s):  
Chaviva Isersky ◽  
Juan Rivera ◽  
Timothy J. Triche ◽  
Henry Metzger
Keyword(s):  
Leukemia Cells ◽  
Rat Basophilic Leukemia Cells ◽  
Basophilic Leukemia
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