Kinetic studies of the role of monovalent cations in the amidolytic activity of activated bovine plasma protein C

Biochemistry ◽  
1982 ◽  
Vol 21 (19) ◽  
pp. 4609-4614 ◽  
Author(s):  
Stephen A. Steiner ◽  
Francis J. Castellino
Keyword(s):  
Plasma Protein ◽  
Protein C ◽  
Kinetic Studies ◽  
Monovalent Cations ◽  
Amidolytic Activity ◽  
Bovine Plasma

Anticoagulant properties of bovine plasma protein C following activation by thrombin

Biochemistry ◽  
1977 ◽  
Vol 16 (26) ◽  
pp. 5824-5831 ◽  
Author(s):  
Walter Kisiel ◽  
William M. Canfield ◽  
Lowell H. Ericsson ◽  
Earl W. Davie
Keyword(s):  
Plasma Protein ◽  
Protein C ◽  
Bovine Plasma

Corrections - Interaction of Calcium with Bovine Plasma Protein C

Biochemistry ◽  
1981 ◽  
Vol 20 (17) ◽  
pp. 5094-5094
Author(s):  
Godfrey Amphlett ◽  
Walter Kisiel ◽  
Francis Castellino
Keyword(s):  
Plasma Protein ◽  
Protein C ◽  
Bovine Plasma

Interaction of calcium with bovine plasma protein C

Biochemistry ◽  
1981 ◽  
Vol 20 (8) ◽  
pp. 2156-2161 ◽  
Author(s):  
Godfrey W. Amphlett ◽  
Walter Kisiel ◽  
Francis J. Castellino
Keyword(s):  
Plasma Protein ◽  
Protein C ◽  
Bovine Plasma

Functional Assays for Protein C Activity and Protein C Inhibitor Activity in Plasma

1989 ◽  
Vol 61 (01) ◽  
pp. 086-092 ◽  
Author(s):  
Margarethe Geiger ◽  
Teresa M White ◽  
John H Griffin
Keyword(s):  
Protein C ◽  
Kinetic Studies ◽  
Microtiter Plate ◽  
Amidolytic Activity ◽  
First Order Kinetics ◽  
Maximal Stimulation ◽  
Dose Dependent Increase ◽  
Dose Dependent ◽  
Protein C Activity

SummaryAn assay system for protein C (PC) activity and PC-inhibitor in plasma was developed. The assay was based on: (1) binding of PC to wells of a microtiter plate coated with a murine monoclonal anti-PC antibody (C3) that did not interfere with the activity or activation of PC; (2) activation of immobilized PC with Protac C; (3) incubation with or without a source of activated PC inhibitor; and (4) measurement of amidolytic activity using the substrate S-2366. The activity assay was specific for PC and sensitive to <1 μl of plasma or 4 ng PC. Inhibition of activated PC by plasma followed pseudo first order kinetics. Heparin caused a dose dependent increase in the inhibition rate with half maximal stimulation at approximately 3 U/ml and maximal stimulation at heparin concentrations ≥10 U/ml. This assay is suitable not only for determination of functional plasma levels of PC and PC inhibitor activities but also for kinetic studies of inhibition of activated PC in complex systems, such as plasma. Studies showed that urokinase interfered with the inhibition of APC by plasma inhibitor(s).

Sign in / Sign up

Export Citation Format

Share Document