Mutations of the Asparagine117Residue of a Receptor Activity-Modifying Protein 1-Dependent Human Calcitonin Gene-Related Peptide Receptor Result in Selective Loss of Function†

Biochemistry ◽  
2001 ◽  
Vol 40 (18) ◽  
pp. 5392-5398 ◽  
Author(s):  
Remo Gujer ◽  
Amaya Aldecoa ◽  
Nicole Bühlmann ◽  
Kerstin Leuthäuser ◽  
Roman Muff ◽  
...  
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Receptor Activity ◽  
Human Calcitonin ◽  
Loss Of Function ◽  
Peptide Receptor ◽  
Related Peptide ◽  
Selective Loss ◽  
Calcitonin Gene

Calcitonin gene related peptide relaxes cholecystokinin-induced contraction in guinea pig gallbladder strips in vitro

1992 ◽  
Vol 70 (12) ◽  
pp. 1571-1575 ◽  
Author(s):  
L. W. Kline ◽  
P. K. T. Pang
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Calcitonin Gene Related Peptide ◽  
Intestinal Smooth Muscle ◽  
Human Calcitonin ◽  
Peptide Receptor ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Dose Dependent

Calcitonin gene related peptide has been shown to relax vascular and intestinal smooth muscle. This study examines the effects of calcitonin gene related peptide on cholecystokinin-induced contraction of guinea pig gallbladder strips in vitro. Calcitonin gene related peptide was found to cause a dose-dependent relaxation of cholecystokinin-induced tension, which was blocked by the calcitonin gene related peptide receptor antagonist human calcitonin gene related peptide8–37. Previous studies demonstrated that calcitonin gene related peptide acted directly on guinea pig gallbladder smooth muscle to inhibit acetylcholine- or KCl-induced contraction. The present results further confirm that calcitonin gene related peptide acts directly on the smooth muscle. In addition, the use of L-NG-nitroarginine methyl ester, glibenclamide, and other agents strongly suggests that calcitonin gene related peptide also acts by way of the nonadrenergic noncholinergic nervous system, to induce the relaxation of cholecystokinin-induced contraction observed in the guinea pig gallbladder strips.Key words: calcitonin gene related peptide, gallbladder, cholecystokinin.

Distribution of CGRP and CGRP receptor components in the rat brain

Cephalalgia ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 342-353 ◽  
Author(s):  
Karin Warfvinge ◽  
Lars Edvinsson
Keyword(s):  
Rat Brain ◽  
Calcitonin Gene Related Peptide ◽  
Receptor Activity ◽  
Calcitonin Receptor ◽  
Thalamic Nuclei ◽  
Peptide Receptor ◽  
Brain Functions ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Neuronal Processes

Background Calcitonin gene-related peptide and its receptor, consisting of receptor activity-modifying protein 1 and calcitonin receptor-like receptor, are of considerable interest because of the role they play in migraine and recently developed migraine therapies. Methods To better understand the function of this neuropeptide, we used immunohistochemistry to determine a detailed distribution of calcitonin gene-related peptide, receptor activity-modifying protein 1 and calcitonin receptor-like receptor in the rat brain in a region of 0.5–1.5 mm lateral to the midline. We found calcitonin gene-related peptide immunoreactivity in most of the neurons of the cerebral cortex, hippocampus, cerebellum, thalamic nuclei, hypothalamic nuclei and brainstem nuclei. In contrast, receptor activity-modifying protein 1 and calcitonin receptor-like receptor immunoreactivity were found almost exclusively in the neuronal processes in the investigated regions. Conclusion Overall, the degree of expression of calcitonin gene-related peptide and calcitonin gene-related peptide receptor components in the central nervous system is astonishingly complex and suggestive of many different brain functions, including a possible role in migraine. However, currently, the presence of calcitonin gene-related peptide and the nature of its receptors throughout the brain is an enigma yet to be solved.

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