scholarly journals Overexpression and purification of human calcitonin gene-related peptide–receptor component protein in Escherichia coli

2007 ◽  
Vol 52 (1) ◽  
pp. 167-174 ◽  
Author(s):  
Adviye A. Tolun ◽  
Ian M. Dickerson ◽  
Arun Malhotra
Keyword(s):  
Escherichia Coli ◽  
Calcitonin Gene Related Peptide ◽  
Human Calcitonin ◽  
Peptide Receptor ◽  
Related Peptide ◽  
Receptor Component ◽  
Calcitonin Gene ◽  
Component Protein

scholarly journals Testes Exhibit Elevated Expression of Calcitonin Gene-Related Peptide Receptor Component Protein*

Endocrinology ◽  
1999 ◽  
Vol 140 (3) ◽  
pp. 1459-1469 ◽  
Author(s):  
Wayne Balkan ◽  
Edward L. Oates ◽  
Guy A. Howard ◽  
Bernard A. Roos
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Peptide Receptor ◽  
Related Peptide ◽  
Receptor Component ◽  
Calcitonin Gene ◽  
Elevated Expression ◽  
Component Protein

scholarly journals Vascular reactivity to calcitonin gene-related peptide is enhanced in subtotal nephrectomy-salt induced hypertension

2011 ◽  
Vol 301 (3) ◽  
pp. H683-H688 ◽  
Author(s):  
Scott C. Supowit ◽  
Khurshed A. Katki ◽  
Travis W. Hein ◽  
Prakash Gupta ◽  
Lih Kuo ◽  
...  
Keyword(s):  
Mean Arterial Pressure ◽  
Arterial Pressure ◽  
Tap Water ◽  
Calcitonin Gene Related Peptide ◽  
Subtotal Nephrectomy ◽  
Related Peptide ◽  
Receptor Component ◽  
Calcitonin Gene ◽  
Induced Hypertension ◽  
Component Protein

In subtotal nephrectomy (SN)- and salt-induced hypertension, calcitonin gene-related peptide (CGRP) plays a compensatory role to attenuate the blood pressure increase in the absence of an increase in the neuronal synthesis and release of this peptide. Therefore, the purpose of this study was to determine whether the mechanism of this antihypertensive activity is through enhanced sensitivity of the vasculature to the dilator actions of this neuropeptide. Hypertension was induced in Sprague-Dawley rats by SN and 1% saline drinking water. Control rats were sham-operated and given tap water to drink. After 11 days, rats had intravenous (drug administration) and arterial (continuous mean arterial pressure recording) catheters surgically placed and were studied in a conscious unrestrained state. Baseline mean arterial pressure was higher in the SN-salt rats (157 ± 5 mmHg) compared with controls (128 ± 3 mmHg). Administration of CGRP (and adrenomedullin) produced a significantly greater dose-dependent decrease in mean arterial pressure in SN-salt rats compared with controls (∼2.0-fold for both the low and high doses). Interestingly, isolated superior mesenteric arterioles from SN-salt rats were significantly more responsive to the dilator effects of CGRP (but not adenomedullin) than the controls (pEC50, SN-salt, 14.0 ± 0.1 vs. control, 12.0 ± 0.1). Analysis of the CGRP receptor proteins showed that only the receptor component protein was increased significantly in arterioles from SN-salt rats. These data indicate that the compensatory antihypertensive effects of CGRP result from an increased sensitivity of the vasculature to dilator activity of this peptide. The mechanism may be via the upregulation of receptor component protein, thereby providing a more efficient coupling of the receptor to the signal transduction pathways.

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