Age-dependent accumulation of N.epsilon.-(carboxymethyl)lysine and N.epsilon.-(carboxymethyl)hydroxylysine in human skin collagen

Biochemistry ◽  
1991 ◽  
Vol 30 (5) ◽  
pp. 1205-1210 ◽  
Author(s):  
John A. Dunn ◽  
David R. McCance ◽  
Suzanne R. Thorpe ◽  
Timothy J. Lyons ◽  
John W. Baynes
Keyword(s):  
Human Skin ◽  
Skin Collagen ◽  
Age Dependent ◽  
Carboxymethyl Lysine

scholarly journals 2-Aminoadipic acid is a marker of protein carbonyl oxidation in the aging human skin: effects of diabetes, renal failure and sepsis

2007 ◽  
Vol 404 (2) ◽  
pp. 269-277 ◽  
Author(s):  
David R. Sell ◽  
Christopher M. Strauch ◽  
Wei Shen ◽  
Vincent M. Monnier
Keyword(s):  
Renal Failure ◽  
Human Skin ◽  
Protein Carbonyl ◽  
Catalysed Oxidation ◽  
Skin Collagen ◽  
Lysine Residues ◽  
Selective Ion Monitoring ◽  
Carboxymethyl Lysine ◽  
Reliable Marker ◽  
Age Range

We hypothesized that the ϵ-amino group of lysine residues in longlived proteins oxidatively deaminates with age forming the carbonyl compound, allysine (α-aminoadipic acid-δ-semialdehyde), which can further oxidize into 2-aminoadipic acid. In the present study, we measured both products in insoluble human skin collagen from n=117 individuals of age range 10–90 years, of which n=61 and n=56 were non-diabetic and diabetic respectively, and a total of n=61 individuals had either acute or chronic renal failure. Allysine was reduced by borohydride into 6-hydroxynorleucine and both products were measured in acid hydrolysates by selective ion monitoring gas chromatography (GC)-MS. The results showed that 2-aminoadipic acid (P<0.0001), but not 6-hydroxynorleucine (P=0.14), significantly increased with age reaching levels of 1 and 0.3 mmol/mol lysine at late age respectively. Diabetes in the absence of renal failure significantly (P<0.0001) increased 2-aminoadipic acid up to <3 mmol/mol, but not 6-hydroxynorleucine (levels<0.4 mmol/mol, P=0.18). Renal failure even in the absence of diabetes markedly increased levels reaching up to <0.5 and 8 mmol/mol for 6-hydroxynorleucine and 2-aminoadipic acid respectively. Septicaemia significantly (P<0.0001) elevated 2-aminoadipic acid in non-diabetic, but not diabetic individuals, and mildly correlated with other glycoxidation markers, carboxymethyl-lysine and the methylglyoxal-derived products, carboxyethyl-lysine, argpyrimidine and MODIC (methylglyoxal-derived imidazolium cross-link). These results provide support for the presence of metal-catalysed oxidation (the Suyama pathway) in diabetes and the possible activation of myeloperoxidase during sepsis. We conclude that 2-aminoadipic acid is a more reliable marker for protein oxidation than its precursor, allysine. Its mechanism of formation in each of these conditions needs to be elucidated.

Caloric Restriction Decreases Age-Dependent Accumulation of the Glycoxidation Products, Nisin-(Carboxymethyl)lysine and Pentosidine, in Rat Skin Collagen

1995 ◽  
Vol 50A (6) ◽  
pp. B337-B341 ◽  
Author(s):  
W. T. Cefalu ◽  
A. D. Bell-Farrow ◽  
Z. Q. Wang ◽  
W. E. Sonntag ◽  
M.-X. Fu ◽  
...  
Keyword(s):  
Caloric Restriction ◽  
Rat Skin ◽  
Skin Collagen ◽  
Age Dependent ◽  
Carboxymethyl Lysine

Heat-Shock Protein 90 (HSP90) in Age-Dependent Changes in the Fibroblast Number in Human Skin

2020 ◽  
Vol 10 (3) ◽  
pp. 260-265
Author(s):  
A. G. Gunin ◽  
N. N. Golubtzova ◽  
N. K. Kornilova
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Human Skin ◽  
Heat Shock Protein 90 ◽  
Age Dependent

Capturing Age-Dependent Properties of Human Skin Using Magnetorheological Elastomers

2018 ◽  
Author(s):  
Kyle Weaver ◽  
Jeong-Hoi Koo ◽  
Tae-Heon Yang ◽  
Young-Min Kim
Keyword(s):  
Mechanical Properties ◽  
Human Skin ◽  
Skin Testing ◽  
Filler Particle ◽  
Variable Stiffness ◽  
Particle Volume ◽  
Magnetorheological Elastomers ◽  
Age Dependent ◽  
Skin Samples

Artificial and synthetic skins are widely used in the medical field; used in applications ranging from skin grafts to suture training pads. There is a growing need for artificial skins with tunable properties. However, current artificial skins do not take into account the variability of mechanical properties between individual humans as well as the age-dependent properties of human skin. Furthermore, there has been little development in artificial skins based on these properties. Thus, the primary purpose of this research is to develop variable stiffness artificial skin samples using magnetorheological elastomers (MREs) whose properties that can be controlled using external magnetic fields. In this study, multiple MRE skin samples were fabricated with varying filler particle volume contents. Using a precision dynamic mechanical analyzer, a series of indenting experiments were performed on the samples to characterize their mechanical properties. The samples were tested using a spherical indenter that indented a total depth of 1 mm with a speed of 0.01 mm/s and unloaded at the same rate. The results show that the modulus or stiffness increases significantly as the iron percent (w/w) in the sample increases. Additionally, the stiffness of the sample increases proportional to the intensity of the applied external magnetic field. To assess the MRE samples’ variability of properties, the testing results were compared with in vivo human skin testing data. The results show the MRE samples are feasible to represent the age-dependent stiffness demonstrated in in vivo human skin testing. The MRE materials studied will be further studied as a variable-stiffness skin model in medical devices, such as radial pulse simulators.

Analysis of the age-related composition of human skin collagen and collagens synthesized by fibroblast culture

1994 ◽  
Vol 286 (7) ◽  
pp. 391-395 ◽  
Author(s):  
J. Brinckmann ◽  
M. Bodo ◽  
M. Brey ◽  
H. H. Wolff ◽  
P. K. M�ller
Keyword(s):  
Human Skin ◽  
Fibroblast Culture ◽  
Age Related ◽  
Skin Collagen
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