Inhibition of Gene Expression Inside Cells by Peptide Nucleic Acids:  Effect of mRNA Target Sequence, Mismatched Bases, and PNA Length

Biochemistry ◽  
2001 ◽  
Vol 40 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Donald F. Doyle ◽  
Dwaine A. Braasch ◽  
Bethany A. Janowski ◽  
David R. Corey
Keyword(s):  
Gene Expression ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Target Sequence

scholarly journals Use of Peptide Nucleic Acids to Manipulate Gene Expression in the Malaria Parasite Plasmodium falciparum

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86802 ◽  
Author(s):  
Netanel Kolevzon ◽  
Abed Nasereddin ◽  
Shankar Naik ◽  
Eylon Yavin ◽  
Ron Dzikowski
Keyword(s):  
Gene Expression ◽  
Plasmodium Falciparum ◽  
Nucleic Acids ◽  
Malaria Parasite ◽  
Peptide Nucleic Acids ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum

Using peptide nucleic acids as gene-expression modifiers to reduce β-amyloid levels

2002 ◽  
Vol 19 (1-2) ◽  
pp. 71-76 ◽  
Author(s):  
Beth M. McMahon ◽  
Jennifer Stewart ◽  
Abdul Fauq ◽  
Steven Younkin ◽  
Linda Younkin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Β Amyloid

Inhibition of Gene Expression by Peptide Nucleic Acids in Cultured Cells

ChemInform ◽  
2004 ◽  
Vol 35 (9) ◽  
Author(s):  
Susanna Cogoi ◽  
Valentina Rapozzi ◽  
Luigi E. Xodo
Keyword(s):  
Gene Expression ◽  
Nucleic Acids ◽  
Cultured Cells ◽  
Peptide Nucleic Acids

Impact of biophysical parameters on the biological assessment of peptide nucleic acids, antisense inhibitors of gene expression

1995 ◽  
Vol 34 (2) ◽  
pp. 184-195 ◽  
Author(s):  
Stewart A. Noble ◽  
Michele A. Bonham ◽  
John E. Bisi ◽  
David A. Bruckenstein ◽  
Pamela H. Brown ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Biological Assessment ◽  
Biophysical Parameters

scholarly journals SNP Discrimination by Tolane-Modified Peptide Nucleic Acids: Application for the Detection of Drug Resistance in Pathogens

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 769 ◽  
Author(s):  
Kenji Takagi ◽  
Tenko Hayashi ◽  
Shinjiro Sawada ◽  
Miku Okazaki ◽  
Sakiko Hori ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Nucleic Acids ◽  
Base Pair ◽  
Bacterial Infections ◽  
Peptide Nucleic Acids ◽  
Conformational Search ◽  
Target Sequence ◽  
Single Base ◽  
Influenza A H1n1 ◽  
Mismatched Base Pair

During the treatment of viral or bacterial infections, it is important to evaluate any resistance to the therapeutic agents used. An amino acid substitution arising from a single base mutation in a particular gene often causes drug resistance in pathogens. Therefore, molecular tools that discriminate a single base mismatch in the target sequence are required for achieving therapeutic success. Here, we synthesized peptide nucleic acids (PNAs) derivatized with tolane via an amide linkage at the N-terminus and succeeded in improving the sequence specificity, even with a mismatched base pair located near the terminal region of the duplex. We assessed the sequence specificities of the tolane-PNAs for single-strand DNA and RNA by UV-melting temperature analysis, thermodynamic analysis, an in silico conformational search, and a gel mobility shift assay. As a result, all of the PNA-tolane derivatives stabilized duplex formation to the matched target sequence without inducing mismatch target binding. Among the different PNA-tolane derivatives, PNA that was modified with a naphthyl-type tolane could efficiently discriminate a mismatched base pair and be utilized for the detection of resistance to neuraminidase inhibitors of the influenza A/H1N1 virus. Therefore, our molecular tool can be used to discriminate single nucleotide polymorphisms that are related to drug resistance in pathogens.

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