Thermodynamic Properties of the Transition State for the Rate-Limiting Step in the Folding of the .alpha. Subunit of Tryptophan Synthase

Biochemistry ◽  
1994 ◽  
Vol 33 (20) ◽  
pp. 6356-6362 ◽  
Author(s):  
Xiaowu Chen ◽  
C. Robert Matthews
Keyword(s):  
Thermodynamic Properties ◽  
Transition State ◽  
Alpha Subunit ◽  
Tryptophan Synthase ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

DFT study of phenol alkylation with propylene on H-BEA in the absence and presence of water

2021 ◽  
Author(s):  
Sajal Kanti Dutta ◽  
Vishal Agarwal
Keyword(s):  
Transition State ◽  
Transition State Theory ◽  
Rate Coefficient ◽  
Activation Barrier ◽  
State Theory ◽  
Dft Study ◽  
Phenol Alkylation ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

Water reduces the activation barrier of the rate-limiting step of phenol alkylation with propylene in H-BEA. This, in turn, increases the transition-state theory rate coefficient by two orders-of-magnitude, suggesting much faster alkylation.

Transition State of the Rate-Limiting Step of Heat Denaturation of Cry3A δ-Endotoxin†

Biochemistry ◽  
1999 ◽  
Vol 38 (13) ◽  
pp. 4121-4127 ◽  
Author(s):  
Sergey A. Potekhin ◽  
Olga I. Loseva ◽  
Elizabeth I. Tiktopulo ◽  
Anatoly P. Dobritsa
Keyword(s):  
Transition State ◽  
Heat Denaturation ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

scholarly journals Biosynthesis, assembly and secretion of fibrinogen in cultured rat hepatocytes

1988 ◽  
Vol 251 (2) ◽  
pp. 373-377 ◽  
Author(s):  
S Hirose ◽  
K Oda ◽  
Y Ikehara
Keyword(s):  
Rat Hepatocytes ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Gamma Subunit ◽  
Intracellular Pool ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Gamma Subunits ◽  
Cultured Rat Hepatocytes ◽  
Rate Limiting

The biosynthesis, assembly and secretion of fibrinogen were investigated in cultured rat hepatocytes which were incubated with [35S]methionine. When initial rates of the synthesis of three fibrinogen subunits were compared, the A alpha-subunit was found to be synthesized significantly slower than the B beta- and gamma-subunits. Pulse-chase experiments revealed that the secreted fibrinogen contained different proportions of the newly synthesized subunits, depending upon the chase times. Radioactivity in the A alpha subunit, which initially had the highest level of the three, was rapidly decreased in parallel with the chase time. The gamma-subunit had an increasing amount of the radioactivity in the secreted molecule during the chase periods, whereas that in the B beta-subunit was gradually decreased at the later stages of chase. Analysis of intracellular components of fibrinogen confirmed that the nascent A alpha-subunit was most rapidly exhausted, and the gamma-subunit occupied the largest proportion among the non-assembled subunits at later stages of chase. Taken together, these results suggest that the synthesis of A alpha-subunit, which has the lowest rate, could be the rate-limiting step in the production and secretion of fibrinogen in cultured rat hepatocytes, in contrast with what has been proposed for human and rabbit fibrinogen, namely that the synthesis of B beta-subunit is the rate-limiting step. The results also indicate that there is a large intracellular pool of gamma-subunit.

Ornithine Decarboxylase Activity in Cultured Endothelial Cells Stimulated by Serum, Thrombin and Serotonin

1978 ◽  
Vol 39 (02) ◽  
pp. 496-503 ◽  
Author(s):  
P A D’Amore ◽  
H B Hechtman ◽  
D Shepro
Keyword(s):  
Endothelial Cells ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Aortic Endothelial Cells ◽  
Ornithine Decarboxylase Activity ◽  
Rate Limiting Step ◽  
Bovine Aortic Endothelial Cells ◽  
Limiting Step ◽  
Rate Limiting ◽  
Serum Serotonin

SummaryOrnithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.

Dynamics of hepatic and peripheral insulin effects suggest common rate-limiting step in vivo

Diabetes ◽  
1993 ◽  
Vol 42 (2) ◽  
pp. 296-306 ◽  
Author(s):  
D. C. Bradley ◽  
R. A. Poulin ◽  
R. N. Bergman
Keyword(s):  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting
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