Analysis of the binding of 1,3-diacetylchloramphenicol to chloramphenicol acetyltransferase by isotope-edited proton NMR and site-directed mutagenesis

Biochemistry ◽  
1992 ◽  
Vol 31 (35) ◽  
pp. 8191-8195 ◽  
Author(s):  
J. P. Derrick ◽  
L. Y. Lian ◽  
G. C. K. Roberts ◽  
W. V. Shaw
Keyword(s):  
Proton Nmr ◽  
Chloramphenicol Acetyltransferase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Catalytic mechanism of chloramphenicol acetyltransferase investigated by site-directed mutagenesis

1986 ◽  
Vol 14 (6) ◽  
pp. 1227-1228 ◽  
Author(s):  
IAIN A. MURRAY ◽  
ANN LEWENDON ◽  
COLIN KLEANTHOUS ◽  
WILLIAM V. SHAW
Keyword(s):  
Catalytic Mechanism ◽  
Chloramphenicol Acetyltransferase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Abbreviation used: CAT, chloramphenicol acetyltransferase.

Site-directed mutagenesis and proton NMR spectroscopy of an interdomain segment in the pyruvate dehydrogenase multienzyme complex of Escherichia coli

Biochemistry ◽  
1988 ◽  
Vol 27 (1) ◽  
pp. 289-296 ◽  
Author(s):  
Frieda L. Texter ◽  
Sheena E. Radford ◽  
Ernest D. Laue ◽  
Richard N. Perham ◽  
John S. Miles ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nmr Spectroscopy ◽  
Pyruvate Dehydrogenase ◽  
Proton Nmr ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Multienzyme Complex ◽  
Proton Nmr Spectroscopy

Structure-function relationships in human epidermal growth factor studied by site-directed mutagenesis and proton NMR

Biochemistry ◽  
1991 ◽  
Vol 30 (36) ◽  
pp. 8891-8898 ◽  
Author(s):  
Ulrich Hommel ◽  
Timothy J. Dudgeon ◽  
Anthony Fallon ◽  
R. Mark Edwards ◽  
Iain D. Campbell
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Structure Function ◽  
Proton Nmr ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Human Epidermal Growth Factor ◽  
Epidermal Growth

Analysis of Hydrogen Bonding in Enzyme-Substrate Complexes of Chloramphenicol Acetyltransferase by Infrared Spectroscopy and Site-Directed Mutagenesis

Biochemistry ◽  
1994 ◽  
Vol 33 (33) ◽  
pp. 9826-9830 ◽  
Author(s):  
Iain A. Murray ◽  
Jeremy P. Derrick ◽  
Andrew J. White ◽  
Kevin Drabble ◽  
Christopher W. Wharton ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Infrared Spectroscopy ◽  
Chloramphenicol Acetyltransferase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Enzyme Substrate

Proton NMR (500 MHz) identification of aromatic residues of gene 32 protein involved in DNA binding by use of protein containing perdeuterated aromatic residues and by site-directed mutagenesis

Biochemistry ◽  
1986 ◽  
Vol 25 (12) ◽  
pp. 3666-3672 ◽  
Author(s):  
Richard V. Prigodich ◽  
Yousif Shamoo ◽  
Kenneth R. Williams ◽  
John W. Chase ◽  
William H. Konigsberg ◽  
...  
Keyword(s):  
Dna Binding ◽  
Proton Nmr ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Aromatic Residues ◽  
Gene 32
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