Tryptophan Fluorescence of Chloramphenicol Acetyltransferase: Resolution of Individual Excited-State Lifetimes by Site-Directed Mutagenesis and Multifrequency Phase Fluorometry

Biochemistry ◽  
1995 ◽  
Vol 34 (11) ◽  
pp. 3513-3520 ◽  
Author(s):  
Jacqueline Ellis ◽  
Clive R. Bagshaw ◽  
William V. Shaw
Keyword(s):  
Excited State ◽  
Tryptophan Fluorescence ◽  
Chloramphenicol Acetyltransferase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Phase Fluorometry ◽  
Excited State Lifetimes

Catalytic mechanism of chloramphenicol acetyltransferase investigated by site-directed mutagenesis

1986 ◽  
Vol 14 (6) ◽  
pp. 1227-1228 ◽  
Author(s):  
IAIN A. MURRAY ◽  
ANN LEWENDON ◽  
COLIN KLEANTHOUS ◽  
WILLIAM V. SHAW
Keyword(s):  
Catalytic Mechanism ◽  
Chloramphenicol Acetyltransferase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Abbreviation used: CAT, chloramphenicol acetyltransferase.

Dissecting the Catalytic Mechanism of Betaine−HomocysteineS-Methyltransferase by Use of Intrinsic Tryptophan Fluorescence and Site-Directed Mutagenesis†

Biochemistry ◽  
2004 ◽  
Vol 43 (18) ◽  
pp. 5341-5351 ◽  
Author(s):  
Carmen Castro ◽  
Alejandra A. Gratson ◽  
John C. Evans ◽  
Jiri Jiracek ◽  
Michaela Collinsová ◽  
...  
Keyword(s):  
Catalytic Mechanism ◽  
Tryptophan Fluorescence ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Intrinsic Tryptophan Fluorescence

Determination of the excited-state lifetimes of the tryptophan residues in barnase, via multifrequency phase fluorometry of tryptophan mutants

Biochemistry ◽  
1992 ◽  
Vol 31 (3) ◽  
pp. 711-716 ◽  
Author(s):  
K. Willaert ◽  
R. Loewenthal ◽  
J. Sancho ◽  
M. Froeyen ◽  
A. Fersht ◽  
...  
Keyword(s):  
Excited State ◽  
Phase Fluorometry ◽  
Tryptophan Residues ◽  
Excited State Lifetimes

Analysis of Hydrogen Bonding in Enzyme-Substrate Complexes of Chloramphenicol Acetyltransferase by Infrared Spectroscopy and Site-Directed Mutagenesis

Biochemistry ◽  
1994 ◽  
Vol 33 (33) ◽  
pp. 9826-9830 ◽  
Author(s):  
Iain A. Murray ◽  
Jeremy P. Derrick ◽  
Andrew J. White ◽  
Kevin Drabble ◽  
Christopher W. Wharton ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Infrared Spectroscopy ◽  
Chloramphenicol Acetyltransferase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Enzyme Substrate
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