Structure-function analysis of DNA polymerase-.beta. using monoclonal antibodies: identification of a putative nucleotide binding domain

Biochemistry ◽  
1992 ◽  
Vol 31 (34) ◽  
pp. 7989-7997 ◽  
Author(s):  
Anthony J. Recupero ◽  
Diane C. Rein ◽  
Ralph R. Meyer
Keyword(s):  
Monoclonal Antibodies ◽  
Structure Function ◽  
Dna Polymerase ◽  
Function Analysis ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Dna Polymerase Beta ◽  
Nucleotide Binding Domain ◽  
Polymerase Beta

Structure-function analysis of the mammalian DNA polymerase .beta. active site: role of aspartic acid 256, arginine 254, and arginine 258 in nucleotidyl transfer

Biochemistry ◽  
1995 ◽  
Vol 34 (49) ◽  
pp. 15934-15942 ◽  
Author(s):  
Karen L. Menge ◽  
Zdenek Hostomsky ◽  
Beverly R. Nodes ◽  
Geoffrey O. Hudson ◽  
Soheil Rahmati ◽  
...  
Keyword(s):  
Structure Function ◽  
Aspartic Acid ◽  
Dna Polymerase ◽  
Active Site ◽  
Function Analysis ◽  
Dna Polymerase Beta ◽  
Polymerase Beta

scholarly journals Studies of the domain structure of mammalian DNA polymerase beta. Identification of a discrete template binding domain.

1990 ◽  
Vol 265 (4) ◽  
pp. 2124-2131
Author(s):  
A Kumar ◽  
S G Widen ◽  
K R Williams ◽  
P Kedar ◽  
R L Karpel ◽  
...  
Keyword(s):  
Domain Structure ◽  
Dna Polymerase ◽  
Binding Domain ◽  
Dna Polymerase Beta ◽  
Polymerase Beta

scholarly journals Definition of surface-exposed epitopes on the (Ca2+-Mg2+)-ATPase of sarcoplasmic reticulum

1991 ◽  
Vol 279 (1) ◽  
pp. 203-212 ◽  
Author(s):  
R E A Tunwell ◽  
J W Conlan ◽  
I Matthews ◽  
J M East ◽  
A G Lee
Keyword(s):  
Monoclonal Antibodies ◽  
Sarcoplasmic Reticulum ◽  
Polyclonal Antibodies ◽  
Nucleotide Binding ◽  
Binding Domain ◽  
Nucleotide Binding Domain ◽  
Stalk Domain ◽  
Definition Of ◽  
Peptide Antibodies

Epitopes for monoclonal antibodies binding to the native (Ca(2+)-Mg2+)-ATPase have been defined by studying binding to sets of hexameric peptides synthesized on plastic pegs. Epitopes have been confirmed by demonstrating the binding of anti-peptide antibodies to the ATPase. A method is presented for definition of surface-exposed epitopes using polyclonal antibodies. Three surface-exposed epitopes have been defined in the nucleotide-binding domain of the ATPase, suggesting considerable surface exposure of this region. Other surface-exposed epitopes have been located in the region of the fourth stalk domain.

scholarly journals The Asp285 Variant of DNA Polymerase Beta Extends Mispaired Primer Termini via Increased Nucleotide Binding

Biochemistry ◽  
2008 ◽  
Vol 47 (31) ◽  
pp. 8048-8057 ◽  
Author(s):  
Drew L. Murphy ◽  
Jessica Kosa ◽  
Joachim Jaeger ◽  
Joann B. Sweasy
Keyword(s):  
Dna Polymerase ◽  
Nucleotide Binding ◽  
Dna Polymerase Beta ◽  
Polymerase Beta
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