Sequence specificity in triple helix formation: experimental and theoretical studies of the effect of mismatches on triplex stability

Biochemistry ◽  
1991 ◽  
Vol 30 (40) ◽  
pp. 9791-9798 ◽  
Author(s):  
Jean Louis Mergny ◽  
Jian Sheng Sun ◽  
Michel Rougee ◽  
Therese Montenay-Garestier ◽  
Francisca Barcelo ◽  
...  
Keyword(s):  
Triple Helix ◽  
Theoretical Studies ◽  
Sequence Specificity ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Experimental And Theoretical Studies

Formation of stable DNA triplexes

2011 ◽  
Vol 39 (2) ◽  
pp. 629-634 ◽  
Author(s):  
Keith R. Fox ◽  
Tom Brown
Keyword(s):  
Triple Helix ◽  
Low Ph ◽  
Cross Linking ◽  
Duplex Dna ◽  
Sequence Specificity ◽  
Modified Oligonucleotides ◽  
Major Groove ◽  
Dna Triplexes ◽  
Helix Formation ◽  
Triple Helix Formation

Triple-helical nucleic acids are formed by binding an oligonucleotide within the major groove of duplex DNA. These complexes offer the possibility of designing oligonucleotides which bind to duplex DNA with considerable sequence specificity. However, triple-helix formation with natural nucleotides is limited by (i) the requirement for low pH, (ii) the requirement for homopurine target sequences, and (iii) their relatively low affinity. We have prepared modified oligonucleotides to overcome these limitations, including the addition of positive charges to the sugar and/or base, the inclusion of cytosine analogues, the development of nucleosides for recognition of pyrimidine interruptions and the attachment of one or more cross-linking groups. By these means we are able to generate triplexes which have high affinities at physiological pH at sequences that contain pyrimidine interruptions.

scholarly journals A physico-chemical study of triple helix formation by an oligodeoxythymidylate with N3'->P5' phosphoramidate linkages

1997 ◽  
Vol 25 (9) ◽  
pp. 1782-1787 ◽  
Author(s):  
B.-w. Zhou-Sun ◽  
J. Liquier ◽  
E. Taillandier ◽  
J.-s. Sun ◽  
T. Garestier ◽  
...  
Keyword(s):  
Triple Helix ◽  
Chemical Study ◽  
Helix Formation ◽  
Physico Chemical ◽  
Triple Helix Formation

Characterization and Quantification of Triple Helix Formation in Chromosomal DNA

2004 ◽  
Vol 341 (4) ◽  
pp. 979-989 ◽  
Author(s):  
Robert Besch ◽  
Carine Giovannangeli ◽  
Theda Schuh ◽  
Claudia Kammerbauer ◽  
Klaus Degitz
Keyword(s):  
Triple Helix ◽  
Chromosomal Dna ◽  
Helix Formation ◽  
Triple Helix Formation
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