Fluorescence Characterization of Structural Transitions at the Strong Actin Binding Motif in Skeletal Myosin Affinity Labeled at Cysteine 540 with Novel Spectroscopic Cysteaminyl Mixed Disulfides†

Biochemistry ◽  
2000 ◽  
Vol 39 (47) ◽  
pp. 14626-14637 ◽  
Author(s):  
Raoul Bertrand ◽  
Jean Derancourt ◽  
Ridha Kassab
Keyword(s):  
Actin Binding ◽  
Binding Motif ◽  
Structural Transitions ◽  
Skeletal Myosin ◽  
Mixed Disulfides

Characterization of the human nebulette gene: a polymorphism in an actin-binding motif is associated with nonfamilial idiopathic dilated cardiomyopathy

2000 ◽  
Vol 107 (5) ◽  
pp. 440-451 ◽  
Author(s):  
Takuro Arimura ◽  
Takeyuki Nakamura ◽  
Shitoshi Hiroi ◽  
Manatsu Satoh ◽  
Megumi Takahashi ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Actin Binding ◽  
Binding Motif

scholarly journals Identification and Characterization of the Actin-Binding Motif of Phostensin

2012 ◽  
Vol 13 (12) ◽  
pp. 15967-15982 ◽  
Author(s):  
Tzu-Fan Wang ◽  
Ning-Sheng Lai ◽  
Kuang-Yung Huang ◽  
Hsien-Lu Huang ◽  
Ming-Chi Lu ◽  
...  
Keyword(s):  
Actin Binding ◽  
Binding Motif ◽  
Identification And Characterization

scholarly journals The interfaces of actin and Acanthamoeba actobindin. Identification of a new actin-binding motif

1991 ◽  
Vol 266 (23) ◽  
pp. 15427-15431 ◽  
Author(s):  
K. Vancompernolle ◽  
J. Vandekerckhove ◽  
M.R. Bubb ◽  
E.D. Korn
Keyword(s):  
Actin Binding ◽  
Binding Motif

scholarly journals A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology inChlamydomonas reinhardtii

2019 ◽  
Vol 116 (37) ◽  
pp. 18445-18454 ◽  
Author(s):  
Alan K. Itakura ◽  
Kher Xing Chan ◽  
Nicky Atkinson ◽  
Leif Pallesen ◽  
Lianyong Wang ◽  
...  
Keyword(s):  
Surface Area ◽  
Structure And Function ◽  
Binding Motif ◽  
Starch Granules ◽  
Wild Type ◽  
Bisphosphate Carboxylase ◽  
Biophysical Mechanism ◽  
Mechanism Function ◽  
And Function

A phase-separated, liquid-like organelle called the pyrenoid mediates CO2fixation in the chloroplasts of nearly all eukaryotic algae. While most algae have 1 pyrenoid per chloroplast, here we describe a mutant in the model algaChlamydomonasthat has on average 10 pyrenoids per chloroplast. Characterization of the mutant leads us to propose a model where multiple pyrenoids are favored by an increase in the surface area of the starch sheath that surrounds and binds to the liquid-like pyrenoid matrix. We find that the mutant’s phenotypes are due to disruption of a gene, which we call StArch Granules Abnormal 1 (SAGA1) because starch sheath granules, or plates, in mutants lacking SAGA1 are more elongated and thinner than those of wild type. SAGA1 contains a starch binding motif, suggesting that it may directly regulate starch sheath morphology. SAGA1 localizes to multiple puncta and streaks in the pyrenoid and physically interacts with the small and large subunits of the carbon-fixing enzyme Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase), a major component of the liquid-like pyrenoid matrix. Our findings suggest a biophysical mechanism by which starch sheath morphology affects pyrenoid number and CO2-concentrating mechanism function, advancing our understanding of the structure and function of this biogeochemically important organelle. More broadly, we propose that the number of phase-separated organelles can be regulated by imposing constraints on their surface area.

scholarly journals Characterization of a γ-adaptin ear-binding motif in enthoprotin

FEBS Letters ◽  
2003 ◽  
Vol 555 (3) ◽  
pp. 437-442 ◽  
Author(s):  
Sylwia Wasiak ◽  
Alexei Yu. Denisov ◽  
Zhaozhong Han ◽  
Peter A. Leventis ◽  
Elaine de Heuvel ◽  
...  
Keyword(s):  
Binding Motif
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