scholarly journals Time-resolved energy transfer measurements of donor-acceptor distance distributions and intramolecular flexibility of a CCHH zinc finger peptide

Biochemistry ◽  
1993 ◽  
Vol 32 (31) ◽  
pp. 7981-7993 ◽  
Author(s):  
Peggy S. Eis ◽  
Joseph R. Lakowicz
Keyword(s):  
Energy Transfer ◽  
Zinc Finger ◽  
Time Resolved ◽  
Distance Distributions ◽  
Donor Acceptor ◽  
Zinc Finger Peptide

Time-Resolved Measurements of Intramolecular Energy Transfer in Single Donor/Acceptor Dyads

2005 ◽  
Vol 109 (30) ◽  
pp. 6725-6729 ◽  
Author(s):  
G. Hinze ◽  
M. Haase ◽  
F. Nolde ◽  
K. Müllen ◽  
Th. Basché
Keyword(s):  
Energy Transfer ◽  
Intramolecular Energy Transfer ◽  
Time Resolved ◽  
Single Donor ◽  
Donor Acceptor ◽  
Time Resolved Measurements

scholarly journals Improving Lanthanide-Based Resonance Energy Transfer Detection by Increasing Donor-Acceptor Distances

2006 ◽  
Vol 11 (4) ◽  
pp. 439-443 ◽  
Author(s):  
Kurt W. Vogel ◽  
Kevin L. Vedvik
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Established Method ◽  
Time Resolved ◽  
Energy Donor ◽  
Emission Lifetime ◽  
Donor Acceptor ◽  
Organic Fluorophore ◽  
Energy Acceptor

Lanthanide-based resonance energy transfer (LRET) is an established method for measuring or detecting proximity between a luminescent lanthanide (energy donor) and an organic fluorophore (energy acceptor). Because resonance energy transfer is a distance-dependent phenomenon that increases in efficiency to the 6th power of the distance between the donor and the acceptor, assay systems are often designed to minimize donor-acceptor distances. However, the authors show that because of the R6 relationship between transfer efficiency and sensitized emission lifetime, energy transfer can be difficult to measure in a time-gated manner when the donor-acceptor distance is small relative to the Förster radius. In such systems, the advantages inherent in time-resolved, ratiometric measurements are lost but can be regained by designing the system such that the average donor-acceptor distance is increased.

Luminescent Terbium Complexes with Polymer Ligands

2004 ◽  
Vol 69 (2) ◽  
pp. 309-321 ◽  
Author(s):  
Drahomír Výprachtický ◽  
Věra Cimrová ◽  
Stanislav Kukla ◽  
Luďka Machová
Keyword(s):  
Energy Transfer ◽  
Maleic Anhydride ◽  
Diethyl Maleate ◽  
Time Resolved ◽  
Lanthanide Ion ◽  
Transfer Processes ◽  
Donor Acceptor ◽  
Complexing Properties ◽  
Terbium Complexes ◽  
Polymer Ligands

Alternating and statistical copolymers of 9-vinylcarbazole with diethyl fumarate (1), diethyl maleate (2), methacrylic acid (3), maleic anhydride (4), or maleic acid (5) were synthesized and characterized. These copolymers were tested as polymer ligands, that might be able to suppress the environmental vibronic quenching of a lanthanide ion and, simultaneously, to function as energy donors in the ligand-to-metal energy transfer processes. Time-resolved luminescence of a series of [Tb(III)-ligand] complexes in common and deuterated solvents revealed that the complexing properties of copolymers 3 or 5 are stronger than those of 1 or 2. Consequently, the strong binding affinity decreases the ligand-metal (donor-acceptor) distance and gives rise to an efficient ligand-to-metal energy transfer. Thus, the intensities of the long-lived emission (5D4→7F6, 5D4→7F5, 5D4→7F4, 5D4→7F3) of the [Tb(III)-3], [Tb(III)-5], and [Tb(III)-1] or [Tb(III)-2] complexes were found to be eight times, five times, and less than twice that of uncomplexed Tb3+, respectively.

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