Expression of the testis-specific histone H1t gene: evidence for involvement of multiple cis-acting promoter elements

Biochemistry ◽  
1995 ◽  
Vol 34 (38) ◽  
pp. 12461-12469 ◽  
Author(s):  
Steven A. Wolfe ◽  
Jane M. vanWert ◽  
Sidney R. Grimes
Keyword(s):  
Promoter Elements ◽  
Cis Acting ◽  
Histone H1t

scholarly journals Analysis of cis-Acting Elements Required for bfpA Expression in EnteropathogenicEscherichia coli

1998 ◽  
Vol 180 (11) ◽  
pp. 3013-3016 ◽  
Author(s):  
Victor H. Bustamante ◽  
Edmundo Calva ◽  
Jose Luis Puente
Keyword(s):  
Escherichia Coli ◽  
Growth Medium ◽  
Regulatory Region ◽  
Random Mutagenesis ◽  
Ammonium Concentration ◽  
Upstream Regulatory Region ◽  
Transcriptional Activators ◽  
Promoter Elements ◽  
Medium Temperature ◽  
Cis Acting

ABSTRACT bfpA expression in enteropathogenic Escherichia coli is regulated by growth medium, temperature, and ammonium concentration and requires the BfpT protein (also called PerA), a member of the AraC family of transcriptional activators. Site-directed and PCR random mutagenesis, as well as deletion analysis of thebfpA upstream regulatory region, supported assignment of the promoter elements and demonstrated that the cis-acting elements that mediate BfpT-dependent regulation of bfpA are located between positions −85 and −46. Interestingly, this region shares 73% identity with a 40-bp-long AT-rich tract located upstream of the bfpT gene, which is essential for bfpTautoregulation.

Gelatinase A expression in endothelial cells is regulated by at least two cis-acting promoter elements

1999 ◽  
Vol 1428 (2-3) ◽  
pp. 147-160 ◽  
Author(s):  
Kurt D Bottles ◽  
Elizabeth C Bullen ◽  
Dawn L Updike ◽  
Thien-Khai H Vu ◽  
Eric Phelps ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Gelatinase A ◽  
Promoter Elements ◽  
Cis Acting

The role of cis-acting promoter elements in tissue-specific albumin gene expression

Science ◽  
1989 ◽  
Vol 244 (4902) ◽  
pp. 343-346 ◽  
Author(s):  
P Maire ◽  
J Wuarin ◽  
U Schibler
Keyword(s):  
Gene Expression ◽  
Promoter Elements ◽  
Tissue Specific ◽  
Cis Acting ◽  
Albumin Gene

scholarly journals The tissue-specific transcriptional regulation of the megakaryocytic glycoprotein IIb gene is controlled by interactions between a repressor and positive cis-acting elements

Blood ◽  
1996 ◽  
Vol 88 (6) ◽  
pp. 2062-2070 ◽  
Author(s):  
MH Prandini ◽  
F Martin ◽  
D Thevenon ◽  
G Uzan
Keyword(s):  
Transcriptional Activity ◽  
Tissue Specificity ◽  
Specific Interaction ◽  
Specific Marker ◽  
Promoter Elements ◽  
Sv40 Promoter ◽  
Tissue Specific ◽  
Cis Acting ◽  
Hela Cell Lines ◽  
Repressor Activity

Much information on regulation of the transcription of megakaryocytic genes stems from studies on the glycoprotein IIb (GPIIb) gene, an early and specific marker of this lineage. Transcriptional activity is controlled by the association of positive promoter elements corresponding to binding sites for the transcription factor GATA-1 and a member of the Ets family. In the present study, we show that these elements are not directly involved in the control of cell specificity. In contrast, we identified a sequence located between -170 and -73 that exhibited a repressor activity based on an analysis of the transcriptional activity of 5′-deleted GPIIb promoter fragments transfected in the nonhematopoietic HeLa cells. Further analysis of this repressor by substitution mutagenesis of the -139/-63 region showed that bases -120/-116 and -102/-93 were required for full repressor activity. The repressor is able to interact differentially with GPIIb promoter elements active in the megakaryocytic HEL, the erythroid K562, the monocytic U937, or the nonhematopoietic HeLa cell lines, indicating that it controls GPIIb gene tissue specificity. In addition, direct evidence for tissue-specific interaction between this repressor and the GPIIb -598/ -406 enhancer was obtained when these elements were set in the context of a heterologous SV40 promoter. Interestingly, the same repressor element controlling tissue specificity of the GPIIb gene may also control its temporal expression during megakaryocyte differentiation, based on recent evidence obtained by Fong and Santoro (J Biol Chem 269:18441, 1994). Finally, we found that the -120/-116 GPIIb sequence was part of a consensus motif shared by promoters of other megakaryocyte-specific genes, suggesting a common repressor mechanism.

scholarly journals Identification of Cis-Acting Promoter Elements in Cold- and Dehydration-Induced Transcriptional Pathways in Arabidopsis, Rice, and Soybean

DNA Research ◽  
2011 ◽  
Vol 19 (1) ◽  
pp. 37-49 ◽  
Author(s):  
K. Maruyama ◽  
D. Todaka ◽  
J. Mizoi ◽  
T. Yoshida ◽  
S. Kidokoro ◽  
...  
Keyword(s):  
Promoter Elements ◽  
Cis Acting

scholarly journals Maximal stress-induced transcription from the human HSP70 promoter requires interactions with the basal promoter elements independent of rotational alignment.

1990 ◽  
Vol 10 (6) ◽  
pp. 3125-3136 ◽  
Author(s):  
G T Williams ◽  
R I Morimoto
Keyword(s):  
Heat Shock ◽  
Physiological Stress ◽  
Inducible Promoter ◽  
Inducible Expression ◽  
Promoter Elements ◽  
Transient Transfection Assay ◽  
Heat Shock Element ◽  
Cis Acting ◽  
Maximal Stress ◽  
Human Hsp70

Transcription of the human HSP70 gene is regulated by a complex array of cis-acting promoter elements that respond to conditions that include normal conditions of cell growth and induction following physiological stress. We have examined the requirements of the basal and inducible promoter elements by using promoter mutations and a transient transfection assay. Multiple forms of stress-induced transcription, including heat shock and incubation with heavy metals or amino acid analogs, are mediated by a single heat shock element (HSE) between -105 and -91 consisting of three contiguous 5-base-pair units, NGAAN, that are inverted relative to adjacent units. Maximal inducible expression requires a fully functional basal promoter. Spacing mutations which alter the relative helical orientation of adjacent genetic elements have only minimal effects on basal and stress-inducible expression and show no effects of periodicity. In addition, placement of the HSE adjacent to the basal promoter removes the requirements for a fully functional basal promoter for maximal stress-inducible expression. These results suggest that factors bound at the HSE and the basal promoter can function through multiple interactions.

PCR primers targeting cis-acting promoter elements in plants

2012 ◽  
Vol 59 (3) ◽  
pp. 413-418 ◽  
Author(s):  
M. Zhao ◽  
Z. Liu ◽  
S. Chen ◽  
F. Chen ◽  
J. Jiang ◽  
...  
Keyword(s):  
Pcr Primers ◽  
Promoter Elements ◽  
Cis Acting
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