The role of cis-acting promoter elements in tissue-specific albumin gene expression

Science ◽  
1989 ◽  
Vol 244 (4902) ◽  
pp. 343-346 ◽  
Author(s):  
P Maire ◽  
J Wuarin ◽  
U Schibler
Keyword(s):  
Gene Expression ◽  
Promoter Elements ◽  
Tissue Specific ◽  
Cis Acting ◽  
Albumin Gene

scholarly journals Control of yeast gene expression by transposable elements: maximum expression requires a functional Ty activator sequence and a defective Ty promoter.

1988 ◽  
Vol 8 (10) ◽  
pp. 4009-4017 ◽  
Author(s):  
L R Coney ◽  
G S Roeder
Keyword(s):  
Gene Expression ◽  
Adjacent Gene ◽  
Multiple Sequence ◽  
Sequence Element ◽  
Cis Acting ◽  
Ty Elements ◽  
The Relationship ◽  
Maximum Expression ◽  
Modest Effect

Integration of a transposable element adjacent to a gene frequently results in an alteration in expression of the nearby gene. The purpose of our experiments was to identify cis-acting sequences within a yeast transposon (Ty) that are important for expression of the adjacent gene. The role of these sequences in Ty transcription was also analyzed in order to examine the relationship between Ty and adjacent gene expression. Three naturally occurring Ty elements located at the HIS4 locus were examined. These Ty elements differed by multiple sequence changes and had different effects on HIS4 expression. To determine which sequences were important to Ty and HIS4 expression, Ty::lacZ and Ty::HIS4::lacZ fusion genes were constructed and analyzed. Results of these experiments indicated that a sequence element is present in the Ty epsilon region that is necessary for HIS4 expression but which has only a modest effect on Ty transcription. Additionally, a mutation in the Ty promoter region decreased Ty transcription and increased HIS4 expression. The opposite effects of this mutation on Ty and adjacent gene expression were probably caused by promoter competition.

scholarly journals The tissue specific role of SirT1 in joint inflammation and anabolic cartilage gene expression

2015 ◽  
Vol 23 ◽  
pp. A33
Author(s):  
P.K. Sacitharan ◽  
J. Zarebska ◽  
A. Chanalaris ◽  
G. Bou Gharios ◽  
E. J ◽  
...  
Keyword(s):  
Gene Expression ◽  
Joint Inflammation ◽  
Specific Role ◽  
Tissue Specific

scholarly journals Stress affects the epigenetic marks added by natural transposable element insertions in Drosophila melanogaster

2016 ◽  
Author(s):  
Lain Guio ◽  
Cristina Vieira ◽  
Josefa González
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Transposable Element ◽  
Stress Conditions ◽  
Regulatory Sequences ◽  
Epigenetic Marks ◽  
Cis Acting ◽  
Comprehensive Picture ◽  
Epigenetic Gene Regulation

ABSTRACTTransposable elements are emerging as an important source of cis-acting regulatory sequences and epigenetic marks that could influence gene expression. However, few studies have dissected the role of specific transposable element insertions on epigenetic gene regulation. Bari-Jheh is a natural transposon that mediates resistance to oxidative stress by adding cis-regulatory sequences that affect expression of nearby genes. In this work, we integrated publicly available data with chromatin immunoprecipitation experiments to get a more comprehensive picture of Bari-Jheh molecular effects. We showed that Bari-Jheh was enriched for H3K9me3 in nonstress conditions, and for H3K9me3, H3K4me3 and H3K27me3 in oxidative stress conditions, which is consistent with expression changes in adjacent genes. We further showed that under oxidative stress conditions, H3K4me3 and H3K9me3 spread to the promoter region of Jheh1 gene. Finally, another insertion of the Bari1 family was associated with increased H3K27me3 in oxidative stress conditions suggesting that Bari1 histone marks are copy-specific. We concluded that besides adding cis-regulatory sequences, Bari-Jheh influences gene expression by affecting the local chromatin state.

Regulation of plant genes specifically induced in nitrogen-fixing nodules: role of cis-acting elements and trans-acting factors in leghemoglobin gene expression

1989 ◽  
Vol 13 (3) ◽  
pp. 319-325 ◽  
Author(s):  
F. J. de Bruijn ◽  
G. Felix ◽  
B. Grunenberg ◽  
H. J. Hoffmann ◽  
B. Metz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixing ◽  
Cis Acting ◽  
Plant Genes

A modified pod specific promoter for high level heterologous expression of genes in legumes

2019 ◽  
Author(s):  
Aravind Kumar Konda ◽  
Pallavi Singh ◽  
Khela Ram Soren ◽  
Narendra Pratap Singh
Keyword(s):  
Gene Expression ◽  
Regulatory Elements ◽  
Specific Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Cis Acting ◽  
Inducible Promoters ◽  
Expression Of Genes ◽  
Enhanced Expression ◽  
High Level

Promoters are cis-acting regulatory elements that are usually present upstream to the coding sequences and determine the gene expression. Deployment of tissue specific and inducible promoters are constantly increasing for development of successful and stable multiple transgenic plants. To this end, as a strategy for enhanced expression of cis or transgenes, promoter engineering of the native msg promoter from soya bean has been carried out for executing pod specific expression of genes. Cis regulatory elements such as 5’UTR and poly (A) tract have been incorporated for imparting mRNA stability and translational enhancement to generate the modified 1.285 Kb pod specific promoter. Further to attain transcriptional enhancement the modified promoter has been cloned to generate Bi-directional Duplex Promoters (BDDP). The engineered msg promoter gene constructs can be deployed for high level tissue specific gene expression of cis/trans genes along with chosen terminator in chickpea. soybean and other legumes as well.

scholarly journals Deciphering the Role of various cis-acting regulatory elements in controlling SamDC gene expression in Rice

2014 ◽  
Vol 9 (3) ◽  
pp. e28391 ◽  
Author(s):  
Supratim Basu ◽  
Aryadeep Roychoudhury ◽  
Dibyendu N Sengupta
Keyword(s):  
Gene Expression ◽  
Regulatory Elements ◽  
Cis Acting
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