Novel Method for Covalent Fluorescent Labeling of Plasmid DNA That Maintains Structural Integrity of the Plasmid

2000 ◽  
Vol 11 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Carole Neves ◽  
Gerardo Byk ◽  
Virginie Escriou ◽  
Florence Bussone ◽  
Daniel Scherman ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Structural Integrity ◽  
Fluorescent Labeling ◽  
Novel Method

A novel method for reproducible fluorescent labeling of small amounts of antibodies on solid phase

2007 ◽  
Vol 322 (1-2) ◽  
pp. 40-49 ◽  
Author(s):  
Emma Lundberg ◽  
Mårten Sundberg ◽  
Torbjörn Gräslund ◽  
Mathias Uhlén ◽  
Helene Andersson Svahn
Keyword(s):  
Solid Phase ◽  
Fluorescent Labeling ◽  
Novel Method

Dual Fluorescent Labeling Method to Visualize Plasmid DNA Degradation

2009 ◽  
Vol 20 (1) ◽  
pp. 163-169 ◽  
Author(s):  
Charudharshini Srinivasan ◽  
Shafiuddin Siddiqui ◽  
Lawrence K. Silbart ◽  
Fotios Papadimitrakopoulos ◽  
Diane J. Burgess
Keyword(s):  
Plasmid Dna ◽  
Dna Degradation ◽  
Fluorescent Labeling ◽  
Labeling Method

scholarly journals Selective fluorescent labeling of S-nitrosothiols (S-FLOS): A novel method for studying S-nitrosation

Nitric Oxide ◽  
2008 ◽  
Vol 19 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Lakshmi Santhanam ◽  
Marjan Gucek ◽  
Tashalee R. Brown ◽  
Malini Mansharamani ◽  
Sungwoo Ryoo ◽  
...  
Keyword(s):  
Fluorescent Labeling ◽  
Novel Method

scholarly journals Stable Immune Response Induced by Intradermal DNA Vaccination by a Novel Needleless Pyro-Drive Jet Injector

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Chinyang Chang ◽  
Jiao Sun ◽  
Hiroki Hayashi ◽  
Ayano Suzuki ◽  
Yuko Sakaguchi ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Targeted Delivery ◽  
Antigen Expression ◽  
Dna Vaccination ◽  
Effective Delivery ◽  
Novel Method ◽  
Injection Depth ◽  
Dermal Cells ◽  
Rats And Mice ◽  
Dose Dependent

AbstractDNA vaccination can be applied to the treatment of various infectious diseases and cancers; however, technical difficulties have hindered the development of an effective delivery method. The efficacy of a DNA vaccine depends on optimal antigen expression by the injected plasmid DNA. The pyro-drive jet injector (PJI) is a novel system that allows for adjustment of injection depth and may, thus, provide a targeted delivery approach for various therapeutic or preventative compounds. Herein, we investigated its potential for use in delivering DNA vaccines. This study evaluated the optimal ignition powder dosage, as well as its delivery effectiveness in both rat and mouse models, while comparing the results of the PJI with that of a needle syringe delivery system. We found that the PJI effectively delivered plasmid DNA to intradermal regions in both rats and mice. Further, it efficiently transfected plasmid DNA directly into the nuclei, resulting in higher protein expression than that achieved via needle syringe injection. Moreover, results from animal ovalbumin (OVA) antigen induction models revealed that animals receiving OVA expression plasmids (pOVA) via PJI exhibited dose-dependent (10 μg, 60 μg, and 120 μg) production of anti-OVA antibodies; while only low titers (< 1/100) of OVA antibodies were detected when 120 μg of pOVA was injected via needle syringe. Thus, PJI is an effective, novel method for delivery of plasmid DNA into epidermal and dermal cells suggesting its promise as a tool for DNA vaccination.

scholarly journals Development of Weather-Resistant 3D Printed Structures by Multi-Material Additive Manufacturing

2020 ◽  
Vol 4 (3) ◽  
pp. 94 ◽  
Author(s):  
Arash Afshar ◽  
Roy Wood
Keyword(s):  
Mechanical Properties ◽  
Additive Manufacturing ◽  
Structural Integrity ◽  
Low Cost ◽  
Acrylonitrile Butadiene Styrene ◽  
Outdoor Environments ◽  
3D Printed ◽  
Novel Method ◽  
Structural Surface

Additive manufacturing, or 3D printing, has had a big impact on the manufacturing world through its low cost, material recyclability, and fabrication of intricate geometries with a high resolution. Three-dimensionally printed polymer structures in aerospace, marine, construction, and automotive industries are usually intended for service in outdoor environments. During long-term exposures to harsh environmental conditions, the mechanical properties of these structures can be degraded significantly. Developing coating systems for 3D printed parts that protect the structural surface against environmental effects and provide desired surface properties is crucial for the long-term integrity of these structures. In this study, a novel method was presented to create 3D printed structures coated with a weather-resistant material in a single manufacturing operation using multi-material additive manufacturing. One group of specimens was 3D printed from acrylonitrile-butadiene-styrene (ABS) material and the other group was printed from ABS and acrylic-styrene-acrylonitrile (ASA) as a substrate and coating material, respectively. The uncoated ABS specimens suffered significant degradation in the mechanical properties, particularly in the failure strain and toughness, during exposure to UV radiation, moisture, and high temperature. However, the ASA coating preserved the mechanical properties and structural integrity of ABS 3D printed structures in aggressive environments.

scholarly journals Non-Destructive Identification of Fibre Orientation in Multi-Ply Biaxial Laminates Using Contact Temperature Sensors

Sensors ◽  
2020 ◽  
Vol 20 (14) ◽  
pp. 3865 ◽  
Author(s):  
David I. Gillespie ◽  
Andrew W. Hamilton ◽  
Ewan J. McKay ◽  
Brian Neilson ◽  
Robert C. Atkinson ◽  
...  
Keyword(s):  
Material Properties ◽  
Composite Laminates ◽  
Composite Structures ◽  
Structural Integrity ◽  
Fibre Orientation ◽  
Temperature Sensors ◽  
Inspection Method ◽  
Novel Method ◽  
Non Destructive ◽  
Contact Sensors

Fibre orientation within composite structures dictates the material properties of the laminate once cured. The ability to accurately and automatically assess fibre orientation of composite parts is a significant enabler in the goal to optimise the established processes within aftermarket aerospace industries. Incorrect ply lay-up results in a structure with undesirable material properties and as such, has the potential to fail under safe working loads. Since it is necessary to assure structural integrity during re-manufacture and repair assessment, the paper demonstrates a novel method of readily and non-destructively determining fibre orientation throughout multi-ply Biaxial woven composite laminates using point temperature contact sensors and data analysis techniques. Once cured, only the outermost laminates are visible to assess orientation. The inspection method is conducted visually, with reference guides to allow for rapid adoption with minimum training, as well as harnessing established temperature sensors within the Maintenance Repair and Overhaul (MRO) environment. The system is amenable to integration within existing repair/re-manufacture processes without significant impact to process flow. The method is able to identify noisy samples with an accuracy, precision and recall of 0.9, and for synthetically created samples of double the cure ply thickness, a precision of 0.75, a recall of 0.7 and an accuracy of 0.87.

scholarly journals Dynamic shear analysis: a novel method to determine mechanical integrity of normal and torn human acetabular labra

2018 ◽  
Vol 7 (7) ◽  
pp. 440-446 ◽  
Author(s):  
A. K. Woods ◽  
J. Broomfield ◽  
P. Monk ◽  
F. Vollrath ◽  
S. Glyn-Jones
Keyword(s):  
Storage Modulus ◽  
Structural Integrity ◽  
Biomechanical Testing ◽  
Young Patients ◽  
Dynamic Shear ◽  
Mechanical Integrity ◽  
Labral Tears ◽  
Significant Difference ◽  
Novel Method ◽  
End Stage

ObjectivesThe aim of this study was to investigate the structural integrity of torn and non-torn human acetabular labral tissue.MethodsA total of 47 human labral specimens were obtained from a biobank. These included 22 torn specimens and 25 control specimens from patients undergoing total hip arthroplasty with macroscopically normal labra. The specimens underwent dynamic shear analysis using a rheometer to measure storage modulus, as an indicator of structural integrity.ResultsThere was a significant difference in the storage modulus between torn (mean modulus = 2144.08 Pa) and non-torn (3178.1 Pa) labra (p = 0.0001).ConclusionThe acetabular labrum of young patients with a tear has significantly reduced structural integrity compared with a non-torn labrum in older patients with end-stage osteoarthritis. This study contributes to the understanding of the biomechanics of labral tears, and the observation of reduced structural integrity in torn labra may explain why some repairs fail. Our data demonstrate that labral tears probably have a relatively narrow phenotype, presenting a basis for further investigations that will provide quantifiable data to support their classification and a means to develop a standardized surgical technique for their repair. This study also demonstrates the value of novel biomechanical testing methods in investigating pathological tissues of orthopaedic interest. Cite this article: A. K. Woods, J. Broomfield, P. Monk, F. Vollrath, S. Glyn-Jones. Dynamic shear analysis: a novel method to determine mechanical integrity of normal and torn human acetabular labra: Implications for prediction of outcome of repair. Bone Joint Res 2018;7:440–446. DOI: 10.1302/2046-3758.77.BJR-2017-0282.R2.

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