Surface Modification of PAMAM Dendrimers Modulates the Mechanism of Cellular Internalization

2009 ◽  
Vol 20 (4) ◽  
pp. 693-701 ◽  
Author(s):  
Angkana Saovapakhiran ◽  
Antony D’Emanuele ◽  
David Attwood ◽  
Jeffrey Penny
Keyword(s):  
Surface Modification ◽  
Pamam Dendrimers ◽  
Cellular Internalization

scholarly journals Multivalent Lactose–Ferrocene Conjugates Based on Poly (Amido Amine) Dendrimers and Gold Nanoparticles as Electrochemical Probes for Sensing Galectin-3

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 203 ◽  
Author(s):  
Manuel C. Martos-Maldonado ◽  
Indalecio Quesada-Soriano ◽  
Luis García-Fuentes ◽  
Antonio Vargas-Berenguel
Keyword(s):  
Gold Nanoparticles ◽  
Surface Modification ◽  
Cardiac Remodeling ◽  
Building Blocks ◽  
Differential Pulse ◽  
Pamam Dendrimers ◽  
Titration Calorimetry ◽  
Vis Spectroscopy ◽  
Galectin 3 ◽  
Pulse Voltammetry

Galectin-3 is considered a cancer biomarker and bioindicator of fibrosis and cardiac remodeling and, therefore, it is desirable to develop convenient methods for its detection. Herein, an approach based on the development of multivalent electrochemical probes with high galectin-3 sensing abilities is reported. The probes consist of multivalent presentations of lactose–ferrocene conjugates scaffolded on poly (amido amine) (PAMAM) dendrimers and gold nanoparticles. Such multivalent lactose–ferrocene conjugates are synthesized by coupling of azidomethyl ferrocene–lactose building blocks on alkyne-functionalized PAMAM, for the case of the glycodendrimers, and to disulfide-functionalized linkers that are then used for the surface modification of citrate-stabilized gold nanoparticles. The binding and sensing abilities toward galectin-3 of both ferrocene-containing lactose dendrimers and gold nanoparticles have been evaluated by means of isothermal titration calorimetry, UV–vis spectroscopy, and differential pulse voltammetry. The highest sensitivity by electrochemical methods to galectin-3 was shown by lactosylferrocenylated gold nanoparticles, which are able to detect the lectin in nanomolar concentrations.

Enzyme-sensitive cytotoxic peptide–dendrimer conjugates enhance cell apoptosis and deep tumor penetration

2018 ◽  
Vol 6 (3) ◽  
pp. 604-613 ◽  
Author(s):  
Fu-Hua Liu ◽  
Chun-Yuan Hou ◽  
Di Zhang ◽  
Wen-Jing Zhao ◽  
Yong Cong ◽  
...  
Keyword(s):  
Cell Apoptosis ◽  
Pamam Dendrimers ◽  
Cellular Internalization ◽  
Tumor Penetration ◽  
Dendrimer Conjugates ◽  
Cytotoxic Peptide ◽  
Peptide Dendrimer

Cytotoxic peptide conjugated PAMAM dendrimers with MMP2-sensitive PEG for efficient tumor penetration, cellular internalization and mitochondria disruption.

scholarly journals Multivalent Lactose–Ferrocene Conjugates Based on Poly(Amido Amine) Dendrimers and Gold Nanoparticles as Electrochemical Probes for Sensing Galectin-3

2020 ◽  
Author(s):  
Manuel C. Martos-Maldonado ◽  
Indalecio Quesada-Soriano ◽  
Luis García-Fuentes ◽  
Antonio Vargas-Berenguel
Keyword(s):  
Gold Nanoparticles ◽  
Surface Modification ◽  
Cardiac Remodeling ◽  
Building Blocks ◽  
Differential Pulse ◽  
Pamam Dendrimers ◽  
Titration Calorimetry ◽  
Vis Spectroscopy ◽  
Galectin 3 ◽  
Pulse Voltammetry

Galectin-3 is considered a cancer biomarker and bioindicator of fibrosis and cardiac remodeling, and, therefore, it is desirable to develop convenient methods for its detection. Herein, an approach based on the development of multivalent electrochemical probes with high galectin-3 sensing abilities is reported. The probes consist of multivalent presentations of lactose–ferrocene conjugates scaffolded on poly(amido amine) (PAMAM) dendrimers and gold nanoparticles. Such multivalent lactose–ferrocene conjugates are synthesized by coupling of azidomethylferrocene-lactose building blocks on alkyne-functionalized PAMAM, for the case of the glycodendrimers, and to disulfide‐functionalized linkers that are then used for the surface modification of citrate-stabilized gold nanoparticles. The binding and sensing abilities towards galectin 3 of both ferrocene-containing lactose dendrimers and gold nanoparticles have been evaluated by means of isothermal titration calorimetry, UV-vis spectroscopy, and differential pulse voltammetry. The highest sensitivity by electrochemical methods to galectin-3 was shown by lactosylferrocenylated gold nanoparticles, which are able to detect the lectin in nanomolar concentrations.

Dually functionalized dendrimers by temperature-sensitive surface modification and gold nanoparticle loading for biomedical application

RSC Advances ◽  
2014 ◽  
Vol 4 (53) ◽  
pp. 27811-27819 ◽  
Author(s):  
Kenji Kono ◽  
Keishi Takeda ◽  
Xiaojie Li ◽  
Eiji Yuba ◽  
Atsushi Harada ◽  
...  
Keyword(s):  
Surface Modification ◽  
Gold Nanoparticle ◽  
Diethylene Glycol ◽  
Biomedical Application ◽  
Pamam Dendrimers ◽  
Au Nanoparticle ◽  
Temperature Sensitive ◽  
New Type ◽  
Stimuli Sensitive

Stimuli-sensitive dendrimers of a new type were developed through dual functionalization of polyamidoamine (PAMAM) dendrimers with temperature-sensitive surface modification using propoxy diethylene glycol (PDEG) and Au nanoparticle (AuNP) loading.

scholarly journals Nanoparticle Surface Functionalization: How to Improve Biocompatibility and Cellular Internalization

2020 ◽  
Vol 7 ◽  
Author(s):  
Gennaro Sanità ◽  
Barbara Carrese ◽  
Annalisa Lamberti
Keyword(s):  
Surface Modification ◽  
Surface Functionalization ◽  
Surface Composition ◽  
Chemical Properties ◽  
Cellular Internalization ◽  
Key Factors ◽  
Cytotoxic Effects ◽  
Uptake Efficiency ◽  
Nanoparticle Surface ◽  
Physico Chemical

The use of nanoparticles (NP) in diagnosis and treatment of many human diseases, including cancer, is of increasing interest. However, cytotoxic effects of NPs on cells and the uptake efficiency significantly limit their use in clinical practice. The physico-chemical properties of NPs including surface composition, superficial charge, size and shape are considered the key factors that affect the biocompatibility and uptake efficiency of these nanoplatforms. Thanks to the possibility of modifying physico-chemical properties of NPs, it is possible to improve their biocompatibility and uptake efficiency through the functionalization of the NP surface. In this review, we summarize some of the most recent studies in which NP surface modification enhances biocompatibility and uptake. Furthermore, the most used techniques used to assess biocompatibility and uptake are also reported.

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