Combinatorial Synthesis and High-Throughput Screening of Alkyl Amines for Nonviral Gene Delivery

2013 ◽  
Vol 24 (9) ◽  
pp. 1543-1551 ◽  
Author(s):  
Linxian Li ◽  
Fengjian Wang ◽  
Yihang Wu ◽  
Gary Davidson ◽  
Pavel A. Levkin
Keyword(s):  
Gene Delivery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Combinatorial Synthesis ◽  
Nonviral Gene Delivery ◽  
Alkyl Amines

Parallel High-Throughput Screening of Polymer Vectors for Nonviral Gene Delivery: Evaluation of Structure–Property Relationships of Transfection

2013 ◽  
Vol 15 (9) ◽  
pp. 475-482 ◽  
Author(s):  
Alexandra C. Rinkenauer ◽  
Antje Vollrath ◽  
Anja Schallon ◽  
Lutz Tauhardt ◽  
Kristian Kempe ◽  
...  
Keyword(s):  
Gene Delivery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Nonviral Gene Delivery ◽  
Structure Property ◽  
Structure Property Relationships

Combinatorial synthesis and high throughput screening of lipidoids for gene delivery

2015 ◽  
Vol 213 ◽  
pp. e134 ◽  
Author(s):  
Yihang Wu ◽  
Linxian Li ◽  
Pavel A. Levkin ◽  
Gary Davidson
Keyword(s):  
Gene Delivery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Combinatorial Synthesis

Cycloelimination-assisted Combinatorial Synthesis of Diverse Heterocyclic Scaffolds of Chemotherapeutic Values

2019 ◽  
Vol 23 (7) ◽  
pp. 768-808
Author(s):  
Virendra Prasad ◽  
Nidhi Mishra ◽  
Anand K. Agrahari ◽  
Sumit K. Singh ◽  
Prabhu P. Mohapatra ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Solid Phase ◽  
Combinatorial Libraries ◽  
Building Blocks ◽  
Biological Evaluation ◽  
Molecular Structures ◽  
Combinatorial Synthesis ◽  
Drug Discovery And Development ◽  
Great Ease

Recent advances in high-throughput, automated techniques combined with the identification of new therapeutic targets in genome sequencing and molecular biology have generated a need for a large collection of diverse heterocyclic scaffolds. This inspires toward the development of novel reaction sequences and linking strategies to generate libraries of diverse simple to complex heterocyclic systems. In this regard, combinatorial chemistry has emerged as an excellent technology platform for the rapid assembly of building blocks to synthesize complex molecular structures with great ease in a few synthetic steps. By means of the implementation of high-throughput screening for the biological evaluation of hits and leads, combinatorial libraries have become important assets in drug discovery and development. In the last two decades, the cyclorelease strategy that minimizes the chemical and tethering implications by releasing the intact desired target molecule in the final step of reaction has attracted much attention. Recently, a particular interest is developing in linking strategies, where loading and cleavage steps contribute to the complexity of the target structure rather than only extraneous manipulations. This review summarises the practical and high-yielding approaches of solid phase combinatorial synthesis for diverse high-purity heterocyclic skeletons of pharmacological importance involving the cycloelimination strategy.

High Throughput Screening of Complex Hydrides for Hydrogen Storage

2005 ◽  
Vol 885 ◽  
Author(s):  
Gregory Lewis ◽  
J. W. Adriaan Sachtler ◽  
John J. Low ◽  
David A. Lesch ◽  
Syed A. Faheem ◽  
...  
Keyword(s):  
Hydrogen Storage ◽  
Molecular Mechanics ◽  
High Throughput ◽  
High Throughput Screening ◽  
Metal Hydrides ◽  
Combinatorial Synthesis ◽  
Established Method ◽  
Mild Conditions ◽  
Complex Hydrides ◽  
Combinatorial Methods

ABSTRACTThe discovery that dopants, such as Ti, cause NaAlH4 to reversibly desorb H2 at mild conditions has spurred a great deal of research into complex metal hydrides. However, no complex hydride meets the targets for automotive hydrogen storage. Our approach is to accelerate the rate of discovery of improved hydrides and dopants through the combination of Virtual High Throughput Screening (VHTS) and Combinatorial Synthesis and Screening (CSS). Our CSS methods will allow us to screen thousands of samples in a year. These samples will be prepared by ball milling mixtures of hydrides and dopants similar to the established method of preparing Ti doped NaAlH4. VHTS exploits a molecular mechanics method to screen a thousand phases in a month. The combination of combinatorial methods and VHTS will help us discover the most promising complex hydrides for hydrogen storage. We will show the results of our medium throughput CSS and VHTS as applied to the NaAlH4–LiAlH4 – Mg(AlH4)2 mixed alanate compositions.

scholarly journals High-throughput microfluidic platform for adherent single cells non-viral gene delivery

RSC Advances ◽  
2015 ◽  
Vol 5 (7) ◽  
pp. 5087-5095 ◽  
Author(s):  
P. Occhetta ◽  
C. Malloggi ◽  
A. Gazaneo ◽  
A. Redaelli ◽  
G. Candiani ◽  
...  
Keyword(s):  
Gene Delivery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Single Cells ◽  
Viral Gene ◽  
Single Cell Level ◽  
Cell Level ◽  
Microfluidic Platform ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

Microfluidic platform for the high-throughput screening of gene delivery vectors and optimization of the transfection protocols at a single cell level.

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