scholarly journals High-throughput microfluidic platform for adherent single cells non-viral gene delivery

RSC Advances ◽  
2015 ◽  
Vol 5 (7) ◽  
pp. 5087-5095 ◽  
Author(s):  
P. Occhetta ◽  
C. Malloggi ◽  
A. Gazaneo ◽  
A. Redaelli ◽  
G. Candiani ◽  
...  
Keyword(s):  
Gene Delivery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Single Cells ◽  
Viral Gene ◽  
Single Cell Level ◽  
Cell Level ◽  
Microfluidic Platform ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

Microfluidic platform for the high-throughput screening of gene delivery vectors and optimization of the transfection protocols at a single cell level.

Development of a microfluidic platform for high-throughput screening of non-viral gene delivery vectors

2017 ◽  
Vol 115 (3) ◽  
pp. 775-784 ◽  
Author(s):  
Elisa Giupponi ◽  
Roberta Visone ◽  
Paola Occhetta ◽  
Federica Colombo ◽  
Marco Rasponi ◽  
...  
Keyword(s):  
Gene Delivery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Viral Gene ◽  
Microfluidic Platform ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

Recent advances in the analysis full/empty capsid ratio and genome integrity of adeno-associated virus (AAV) gene delivery vectors

2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Analytical Techniques ◽  
Viral Gene ◽  
Adeno Associated Virus ◽  
Gene Delivery Vectors ◽  
Empty Capsid ◽  
Purification Methods ◽  
Viral Gene Delivery

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.

scholarly journals Cationic lipids with a cyclen headgroup: synthesis and structure–activity relationship studies as non-viral gene vectors

RSC Advances ◽  
2017 ◽  
Vol 7 (30) ◽  
pp. 18681-18689 ◽  
Author(s):  
De-Chun Chang ◽  
Yi-Mei Zhang ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
Xiao-Qi Yu
Keyword(s):  
Gene Delivery ◽  
Structure Activity Relationship ◽  
Cationic Lipids ◽  
Activity Relationship ◽  
Viral Gene ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Gene Delivery Vectors ◽  
Gene Vectors ◽  
Viral Gene Delivery

The structure–activity relationships of cyclen-based cationic lipids as non-viral gene delivery vectors were studied and clarified.

Cholesterol derived cationic lipids as potential non-viral gene delivery vectors and their serum compatibility

2016 ◽  
Vol 26 (10) ◽  
pp. 2401-2407 ◽  
Author(s):  
Jia Ju ◽  
Meng-Lei Huan ◽  
Ning Wan ◽  
Yi-Lin Hou ◽  
Xi-Xi Ma ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Lipids ◽  
Viral Gene ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

Biotinylated Cyclen-Contained Cationic Lipids as Non-Viral Gene Delivery Vectors

2013 ◽  
Vol 82 (4) ◽  
pp. 376-383 ◽  
Author(s):  
Qiang Liu ◽  
Wen-Jing Yi ◽  
Yi-Mei Zhang ◽  
Ji Zhang ◽  
Liandi Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Lipids ◽  
Viral Gene ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

RGD peptide-based non-viral gene delivery vectors targeting integrin αvβ3 for cancer therapy

2018 ◽  
Vol 27 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Shuang Fu ◽  
Xiaodong Xu ◽  
Yu Ma ◽  
Shubiao Zhang ◽  
Shufen Zhang
Keyword(s):  
Cancer Therapy ◽  
Gene Delivery ◽  
Integrin Αvβ3 ◽  
Rgd Peptide ◽  
Viral Gene ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

scholarly journals Optofluidic lab-on-chips for high throughput 3D imaging of cells and tissues

2019 ◽  
Vol 215 ◽  
pp. 11002
Author(s):  
Federico Sala ◽  
Petra Paiè ◽  
Roberto Memeo ◽  
Roberto Osellame ◽  
Andrea Farina ◽  
...  
Keyword(s):  
High Throughput ◽  
Chemical Etching ◽  
3D Imaging ◽  
Single Cells ◽  
Three Dimensions ◽  
Single Cell Level ◽  
Cell Level ◽  
Optical Components ◽  
Femtosecond Laser Micromachining ◽  
On Chip

Optofludic lab-on-chips are compact microsystems that allow the manipulation, the analysis and the imaging of cells with high throughput even at the single cell level. The optofluidic microscopes on chip here presented are fabricated by femtosecond laser micromachining followed by chemical etching. The technique is ideally suited for rapid prototyping and allows 3D geometries and perfect alignment between microfluidic and optical components networks. The devices have been validated on cancer cell lines by imaging in three dimensions both clusters of cells growing on spheres and single cells.

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