Molecularly Imprinted Protein Recognition Cavities Bearing Exchangeable Binding Sites for Postimprinting Site-Directed Introduction of Reporter Molecules for Readout of Binding Events

2014 ◽  
Vol 6 (22) ◽  
pp. 20003-20009 ◽  
Author(s):  
Hirobumi Sunayama ◽  
Toshifumi Takeuchi
Keyword(s):  
Binding Sites ◽  
Protein Recognition ◽  
Molecularly Imprinted

scholarly journals Molecular Recognition: Perspective and a New Approach

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2757
Author(s):  
W. Rudolf Seitz ◽  
Casey J. Grenier ◽  
John R. Csoros ◽  
Rongfang Yang ◽  
Tianyu Ren
Keyword(s):  
Molecular Recognition ◽  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Binding Kinetics ◽  
Molecularly Imprinted ◽  
New Approach ◽  
Imprinted Polymers ◽  
High Affinity ◽  
Water Blocking ◽  
High Level

This perspective presents an overview of approaches to the preparation of molecular recognition agents for chemical sensing. These approaches include chemical synthesis, using catalysts from biological systems, partitioning, aptamers, antibodies and molecularly imprinted polymers. The latter three approaches are general in that they can be applied with a large number of analytes, both proteins and smaller molecules like drugs and hormones. Aptamers and antibodies bind analytes rapidly while molecularly imprinted polymers bind much more slowly. Most molecularly imprinted polymers, formed by polymerizing in the presence of a template, contain a high level of covalent crosslinker that causes the polymer to form a separate phase. This results in a material that is rigid with low affinity for analyte and slow binding kinetics. Our approach to templating is to use predominantly or exclusively noncovalent crosslinks. This results in soluble templated polymers that bind analyte rapidly with high affinity. The biggest challenge of this approach is that the chains are tangled when the templated polymer is dissolved in water, blocking access to binding sites.

Molecularly imprinted fluorescent and colorimetric sensor based on TiO2@Cu(OH)2 nanoparticle autocatalysis for protein recognition

2013 ◽  
Vol 1 (9) ◽  
pp. 1256 ◽  
Author(s):  
Yanxia Li ◽  
Yujun Li ◽  
Lihua Huang ◽  
Qiu Bin ◽  
Zhenyu Lin ◽  
...  
Keyword(s):  
Colorimetric Sensor ◽  
Protein Recognition ◽  
Molecularly Imprinted

Post-Imprinting-Modified Molecularly Imprinted Nanocavities with Two Synergetic, Orthogonal, Glycoprotein-Binding Sites to Transduce Binding Events into Fluorescence Changes

ChemNanoMat ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Takahiro Morishige ◽  
Eri Takano ◽  
Hirobumi Sunayama ◽  
Yukiya Kitayama ◽  
Toshifumi Takeuchi
Keyword(s):  
Binding Sites ◽  
Molecularly Imprinted

Application of Response Surface Methodology to Synthesize Appropriate Molecularly Imprinted Polymer for Diazinon

2014 ◽  
Vol 605 ◽  
pp. 67-70 ◽  
Author(s):  
Mohsen Rahiminezhad ◽  
Seyed Jamaleddin Shahtaheri ◽  
Mohammad Reza Ganjali ◽  
Abbas Rahimi Rahimi Forushani
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymer ◽  
Binding Sites ◽  
Capillary Electrochromatography ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Molecular Imprinting Technology ◽  
Cross Linker

Molecular imprinting technology has become an interesting research area to the preparation of specific sorbent material for environmental and occupational sample preparation techniques (1). In the molecular imprinting technology, specific binding sites have been formed in polymeric matrix, which often have an affinity and selectivity similar to antibody-antigen systems (2). In molecular imprinted technology, functional monomers are arranged in a complementary configuration around a template molecule, then, cross-linker and solvent are also added and the mixture is treated to give a porous material containing nono-sized binding sites. After extraction of the template molecule by washing, vacant imprinted sites will be left in polymer, which are available for rebinding of the template or its structural analogue (3). The stability, convention of preparation and low cost of these materials make them particularly attractive (4). These synthetic materials have been used for capillary electrochromatography (5), chromatography columns (6), sensors (7), and catalyze system (8). Depending on the molecular imprinting approach, different experimental variables such as the type and amounts of functional monomers, porogenic solvent, initiator, monomer to cross-linker ratio, temperature, and etc may alter the properties of the final polymeric materials. In this work, chemometric approach based on Central Composite Design (CCD) was used to design the experiments as well as to find the optimum conditions for preparing appropriate diazinon molecularly imprinted polymer.

Preparation of a novel supermacroporous molecularly imprinted cryogel membrane with a specific ionic liquid for protein recognition and permselectivity

2018 ◽  
Vol 135 (41) ◽  
pp. 46740 ◽  
Author(s):  
Jie-Ping Fan ◽  
Feng-Yan Zhang ◽  
Xue-Meng Yang ◽  
Xue-Hong Zhang ◽  
Ya-Hui Cao ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Protein Recognition ◽  
Molecularly Imprinted

scholarly journals Polymer composition primarily determines the protein recognition characteristics of molecularly imprinted hydrogels

2020 ◽  
Vol 8 (34) ◽  
pp. 7685-7695
Author(s):  
Abhijeet K. Venkataraman ◽  
John R. Clegg ◽  
Nicholas A. Peppas
Keyword(s):  
Molecular Recognition ◽  
Molecular Imprinting ◽  
Material Composition ◽  
Polymer Composition ◽  
Protein Recognition ◽  
Molecularly Imprinted

A hydrogel's molecular recognition properties are determined by the material composition, and are minimally influenced by molecular imprinting.

scholarly journals Recent advances in green reagents for molecularly imprinted polymers

RSC Advances ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 311-327 ◽  
Author(s):  
Xi Wu ◽  
Jiajun Du ◽  
Mengyao Li ◽  
Lintao Wu ◽  
Chun Han ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Recent Advances ◽  
Green Reagents

Molecularly imprinted polymers (MIPs) are tailor-made materials with special binding sites.

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