scholarly journals Effect of Surface Charge on the Cellular Uptake and Cytotoxicity of Fluorescent Labeled Cellulose Nanocrystals

2010 ◽  
Vol 2 (10) ◽  
pp. 2924-2932 ◽  
Author(s):  
Khaled A. Mahmoud ◽  
Jimmy A. Mena ◽  
Keith B. Male ◽  
Sabahudin Hrapovic ◽  
Amine Kamen ◽  
...  
Keyword(s):  
Surface Charge ◽  
Cellular Uptake ◽  
Cellulose Nanocrystals ◽  
Effect Of Surface

scholarly journals EFFECTS OF POLYMERIC NANOPARTICLE SURFACE PROPERTIES ON INTERACTION WITH BRAIN TUMOR ENVIRONMENT

Nano LIFE ◽  
2013 ◽  
Vol 03 (04) ◽  
pp. 1343003 ◽  
Author(s):  
BRANDON MATTIX ◽  
THOMAS MOORE ◽  
OLGA UVAROV ◽  
SAMUEL POLLARD ◽  
LAUREN O'DONNELL ◽  
...  
Keyword(s):  
Human Brain ◽  
Surface Charge ◽  
Cellular Uptake ◽  
Drug Clearance ◽  
Cellular Interaction ◽  
Normal Tissues ◽  
Poly Ethylene ◽  
Uptake Studies ◽  
Effect Of Surface

Current chemotherapy treatments are limited by poor drug solubility, rapid drug clearance and systemic side effects. Additionally, drug penetration into solid tumors is limited by physical diffusion barriers [e.g., extracellular matrix (ECM)]. Nanoparticle (NP) blood circulation half-life, biodistribution and ability to cross extracellular and cellular barriers will be dictated by NP composition, size, shape and surface functionality. Here, we investigated the effect of surface charge of poly(lactide)-poly(ethylene glycol) NPs on mediating cellular interaction. Polymeric NPs of equal sizes were used that had two different surface functionalities: negatively charged carboxyl ( COOH ) and neutral charged methoxy ( OCH 3). Cellular uptake studies showed significantly higher uptake in human brain cancer cells compared to noncancerous human brain cells, and negatively charged COOH NPs were uptaken more than neutral OCH 3 NPs in 2D culture. NPs were also able to load and control the release of paclitaxel (PTX) over 19 days. Toxicity studies in U-87 glioblastoma cells showed that PTX-loaded NPs were effective drug delivery vehicles. Effect of surface charge on NP interaction with the ECM was investigated using collagen in a 3D cellular uptake model, as collagen content varies with the type of cancer and the stage of the disease compared to normal tissues. Results demonstrated that NPs can effectively diffuse across an ECM barrier and into cells, but NP mobility is dictated by surface charge. In vivo biodistribution of OCH 3 NPs in intracranial tumor xenografts showed that NPs more easily accumulated in tumors with less collagen. These results indicate that a robust understanding of NP interaction with various tumor environments can lead to more effective patient-tailored therapies.

Effect of surface charge on the cellular uptake of fluorescent magnetic nanoparticles

2012 ◽  
Vol 14 (10) ◽  
Author(s):  
Slavko Kralj ◽  
Matija Rojnik ◽  
Rok Romih ◽  
Marko Jagodič ◽  
Janko Kos ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Surface Charge ◽  
Cellular Uptake ◽  
Effect Of Surface

scholarly journals Effect of surface charge of polymeric micelles on in vitro cellular uptake

2013 ◽  
Vol 31 (9) ◽  
pp. 1299-1309 ◽  
Author(s):  
Dan-hua Zhou ◽  
Jie Zhang ◽  
Guan Zhang ◽  
Zhi-hua Gan
Keyword(s):  
Surface Charge ◽  
Cellular Uptake ◽  
Polymeric Micelles ◽  
Effect Of Surface

scholarly journals Effect of Surface Charge on Surface-Initiated Atom Transfer Radical Polymerization from Cellulose Nanocrystals in Aqueous Media

2016 ◽  
Vol 17 (4) ◽  
pp. 1404-1413 ◽  
Author(s):  
Justin O. Zoppe ◽  
Xingyu Xu ◽  
Cindy Känel ◽  
Paola Orsolini ◽  
Gilberto Siqueira ◽  
...  
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Surface Charge ◽  
Radical Polymerization ◽  
Cellulose Nanocrystals ◽  
Aqueous Media ◽  
Atom Transfer ◽  
Effect Of Surface

The effect of surface charge on cellular uptake and inflammatory behavior of carbon dots

2020 ◽  
Vol 35 ◽  
pp. 100243 ◽  
Author(s):  
Muhammad Usman ◽  
Yumna Zaheer ◽  
Muhammad Rizwan Younis ◽  
Ruken Esra Demirdogen ◽  
Syed Zajif Hussain ◽  
...  
Keyword(s):  
Surface Charge ◽  
Cellular Uptake ◽  
Carbon Dots ◽  
Effect Of Surface

scholarly journals Charge reversal nano-systems for tumor therapy

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Peng Zhang ◽  
Daoyuan Chen ◽  
Lin Li ◽  
Kaoxiang Sun
Keyword(s):  
Surface Charge ◽  
Cellular Uptake ◽  
Blood Circulation ◽  
Tumor Therapy ◽  
Polymeric Materials ◽  
Antitumor Effects ◽  
Charge Reversal ◽  
Physiological Conditions ◽  
Positively Charged ◽  
Effect Of Surface

AbstractSurface charge of biological and medical nanocarriers has been demonstrated to play an important role in cellular uptake. Owing to the unique physicochemical properties, charge-reversal delivery strategy has rapidly developed as a promising approach for drug delivery application, especially for cancer treatment. Charge-reversal nanocarriers are neutral/negatively charged at physiological conditions while could be triggered to positively charged by specific stimuli (i.e., pH, redox, ROS, enzyme, light or temperature) to achieve the prolonged blood circulation and enhanced tumor cellular uptake, thus to potentiate the antitumor effects of delivered therapeutic agents. In this review, we comprehensively summarized the recent advances of charge-reversal nanocarriers, including: (i) the effect of surface charge on cellular uptake; (ii) charge-conversion mechanisms responding to several specific stimuli; (iii) relation between the chemical structure and charge reversal activity; and (iv) polymeric materials that are commonly applied in the charge-reversal delivery systems. Graphical Abstract

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