Optimal Ratio of Carbon Flux between Glycolysis and the Pentose Phosphate Pathway for Amino Acid Accumulation in Corynebacterium glutamicum

2020 ◽  
Vol 9 (7) ◽  
pp. 1615-1622
Author(s):  
Katsuki Murai ◽  
Daisuke Sasaki ◽  
Shunsuke Kobayashi ◽  
Akira Yamaguchi ◽  
Hiroto Uchikura ◽  
...  
Keyword(s):  
Amino Acid ◽  
Corynebacterium Glutamicum ◽  
Pentose Phosphate Pathway ◽  
Carbon Flux ◽  
Pentose Phosphate ◽  
Optimal Ratio ◽  
Acid Accumulation ◽  
Amino Acid Accumulation

Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum

2020 ◽  
Vol 9 (4) ◽  
pp. 814-826
Author(s):  
Shunsuke Kobayashi ◽  
Hideo Kawaguchi ◽  
Tomokazu Shirai ◽  
Kazuaki Ninomiya ◽  
Kenji Takahashi ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Pentose Phosphate Pathway ◽  
Carbon Flux ◽  
Pentose Phosphate ◽  
Metabolic Switch

scholarly journals Adaptation of central metabolite pools to variations in growth rate and cultivation conditions in Saccharomyces cerevisiae

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Kanhaiya Kumar ◽  
Vishwesh Venkatraman ◽  
Per Bruheim
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Amino Acid ◽  
Growth Rates ◽  
Pentose Phosphate Pathway ◽  
Pentose Phosphate ◽  
Relative Reduction ◽  
Central Metabolism ◽  
Cultivation Conditions ◽  
Biological Studies

Abstract Background Saccharomyces cerevisiae is a well-known popular model system for basic biological studies and serves as a host organism for the heterologous production of commercially interesting small molecules and proteins. The central metabolism is at the core to provide building blocks and energy to support growth and survival in normal situations as well as during exogenous stresses and forced heterologous protein production. Here, we present a comprehensive study of intracellular central metabolite pool profiling when growing S. cerevisiae on different carbon sources in batch cultivations and at different growth rates in nutrient-limited glucose chemostats. The latest versions of absolute quantitative mass spectrometry-based metabolite profiling methodology were applied to cover glycolytic and pentose phosphate pathway metabolites, tricarboxylic acid cycle (TCA), complete amino acid, and deoxy-/nucleoside phosphate pools. Results Glutamate, glutamine, alanine, and citrate were the four most abundant metabolites for most conditions tested. The amino acid is the dominant metabolite class even though a marked relative reduction compared to the other metabolite classes was observed for nitrogen and phosphate limited chemostats. Interestingly, glycolytic and pentose phosphate pathway (PPP) metabolites display the largest variation among the cultivation conditions while the nucleoside phosphate pools are more stable and vary within a closer concentration window. The overall trends for glucose and nitrogen-limited chemostats were increased metabolite pools with the increasing growth rate. Next, comparing the chosen chemostat reference growth rate (0.12 h−1, approximate one-fourth of maximal unlimited growth rate) illuminates an interesting pattern: almost all pools are lower in nitrogen and phosphate limited conditions compared to glucose limitation, except for the TCA metabolites citrate, isocitrate and α-ketoglutarate. Conclusions This study provides new knowledge-how the central metabolism is adapting to various cultivations conditions and growth rates which is essential for expanding our understanding of cellular metabolism and the development of improved phenotypes in metabolic engineering.

Acidic amino acid accumulation by rat choroid plexus during development

1997 ◽  
Vol 102 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Hameed Al-Sarraf ◽  
Jane E Preston ◽  
Malcolm B Segal
Keyword(s):  
Amino Acid ◽  
Choroid Plexus ◽  
Acidic Amino Acid ◽  
Acid Accumulation ◽  
Amino Acid Accumulation
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