Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing

2020 ◽  
Vol 9 (2) ◽  
pp. 304-315 ◽  
Author(s):  
Zhiqiang Wen ◽  
Rodrigo Ledesma-Amaro ◽  
Minrui Lu ◽  
Mingjie Jin ◽  
Sheng Yang
Keyword(s):  
Metabolic Engineering ◽  
Consolidated Bioprocessing ◽  
Butanol Production ◽  
Clostridium Cellulovorans

scholarly journals Improvedn-Butanol Production fromClostridium cellulovoransby Integrated Metabolic and Evolutionary Engineering

2019 ◽  
Vol 85 (7) ◽  
Author(s):  
Zhiqiang Wen ◽  
Rodrigo Ledesma-Amaro ◽  
Jianping Lin ◽  
Yu Jiang ◽  
Sheng Yang
Keyword(s):  
Metabolic Engineering ◽  
Consolidated Bioprocessing ◽  
Low Cost ◽  
Agricultural Waste ◽  
Evolutionary Engineering ◽  
Butanol Production ◽  
Clostridium Cellulovorans ◽  
Content Type ◽  
Corn Cobs ◽  
Engineered Strain

ABSTRACTClostridium cellulovoransDSM 743B offers potential as a chassis strain for biomass refining by consolidated bioprocessing (CBP). However, itsn-butanol production from lignocellulosic biomass has yet to be demonstrated. This study demonstrates the construction of a coenzyme A (CoA)-dependent acetone-butanol-ethanol (ABE) pathway inC. cellulovoransby introducingadhE1andctfA-ctfB-adcgenes fromClostridium acetobutylicumATCC 824, which enabled it to producen-butanol using the abundant and low-cost agricultural waste of alkali-extracted, deshelled corn cobs (AECC) as the sole carbon source. Then, a novel adaptive laboratory evolution (ALE) approach was adapted to strengthen then-butanol tolerance ofC. cellulovoransto fully utilize itsn-butanol output potential. To further improven-butanol production, both metabolic engineering and evolutionary engineering were combined, using the evolved strain as a host for metabolic engineering. Then-butanol production from AECC of the engineeredC. cellulovoranswas increased 138-fold, from less than 0.025 g/liter to 3.47 g/liter. This method represents a milestone towardn-butanol production by CBP, using a single recombinant clostridium strain. The engineered strain offers a promising CBP-enabling microbial chassis forn-butanol fermentation from lignocellulose.IMPORTANCEDue to a lack of genetic tools,Clostridium cellulovoransDSM 743B has not been comprehensively explored as a putative strain platform forn-butanol production by consolidated bioprocessing (CBP). Based on the previous study of genetic tools, strain engineering ofC. cellulovoransfor the development of a CBP-enabling microbial chassis was demonstrated in this study. Metabolic engineering and evolutionary engineering were integrated to improve then-butanol production ofC. cellulovoransfrom the low-cost renewable agricultural waste of alkali-extracted, deshelled corn cobs (AECC). Then-butanol production from AECC was increased 138-fold, from less than 0.025 g/liter to 3.47 g/liter, which represents the highest titer ofn-butanol produced using a single recombinant clostridium strain by CBP reported to date. This engineered strain serves as a promising chassis forn-butanol production from lignocellulose by CBP.

scholarly journals Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization

2019 ◽  
Vol 13 (2) ◽  
pp. 410-422 ◽  
Author(s):  
Zhiqiang Wen ◽  
Qi Li ◽  
Jinle Liu ◽  
Mingjie Jin ◽  
Sheng Yang
Keyword(s):  
Consolidated Bioprocessing ◽  
Butanol Production

Microbial co-culturing systems: butanol production from organic wastes through consolidated bioprocessing

2018 ◽  
Vol 102 (13) ◽  
pp. 5419-5425 ◽  
Author(s):  
Yujia Jiang ◽  
Ting Zhang ◽  
Jiasheng Lu ◽  
Peter Dürre ◽  
Wenming Zhang ◽  
...  
Keyword(s):  
Consolidated Bioprocessing ◽  
Organic Wastes ◽  
Butanol Production

Consolidated bioprocessing performance of a two‐species microbial consortium for butanol production from lignocellulosic biomass

2020 ◽  
Vol 117 (10) ◽  
pp. 2985-2995 ◽  
Author(s):  
Yujia Jiang ◽  
Yang Lv ◽  
Ruofan Wu ◽  
Jiasheng Lu ◽  
Weiliang Dong ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Microbial Consortium ◽  
Consolidated Bioprocessing ◽  
Butanol Production

scholarly journals Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shuobo Shi ◽  
Tong Si ◽  
Zihe Liu ◽  
Hongfang Zhang ◽  
Ee Lui Ang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Butanol Production

Metabolic engineering ofClostridium acetobutylicumM5 for highly selective butanol production

2009 ◽  
Vol 4 (10) ◽  
pp. 1432-1440 ◽  
Author(s):  
Jin Young Lee ◽  
Yu-Sin Jang ◽  
Joungmin Lee ◽  
Eleftherios Terry Papoutsakis ◽  
Sang Yup Lee
Keyword(s):  
Metabolic Engineering ◽  
Butanol Production
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