Consolidated bioprocessing performance of a two‐species microbial consortium for butanol production from lignocellulosic biomass

2020 ◽  
Vol 117 (10) ◽  
pp. 2985-2995 ◽  
Author(s):  
Yujia Jiang ◽  
Yang Lv ◽  
Ruofan Wu ◽  
Jiasheng Lu ◽  
Weiliang Dong ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Microbial Consortium ◽  
Consolidated Bioprocessing ◽  
Butanol Production

scholarly journals Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Chaofeng Li ◽  
Xiaofeng Lin ◽  
Xing Ling ◽  
Shuo Li ◽  
Hao Fang
Keyword(s):  
Acid Production ◽  
Microbial Consortium ◽  
Consolidated Bioprocessing ◽  
High Titer ◽  
Value Added ◽  
Superior Performance ◽  
Glucaric Acid ◽  
Practical Applications ◽  
Work Distribution ◽  
Rut C30

Abstract Background The biomanufacturing of d-glucaric acid has attracted increasing interest because it is one of the top value-added chemicals produced from biomass. Saccharomyces cerevisiae is regarded as an excellent host for d-glucaric acid production. Results The opi1 gene was knocked out because of its negative regulation on myo-inositol synthesis, which is the limiting step of d-glucaric acid production by S. cerevisiae. We then constructed the biosynthesis pathway of d-glucaric acid in S. cerevisiae INVSc1 opi1Δ and obtained two engineered strains, LGA-1 and LGA-C, producing record-breaking titers of d-glucaric acid: 9.53 ± 0.46 g/L and 11.21 ± 0.63 g/L d-glucaric acid from 30 g/L glucose and 10.8 g/L myo-inositol in fed-batch fermentation mode, respectively. However, LGA-1 was preferable because of its genetic stability and its superior performance in practical applications. There have been no reports on d-glucaric acid production from lignocellulose. Therefore, the biorefinery processes, including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) were investigated and compared. CBP using an artificial microbial consortium composed of Trichoderma reesei (T. reesei) Rut-C30 and S. cerevisiae LGA-1 was found to have relatively high d-glucaric acid titers and yields after 7 d of fermentation, 0.54 ± 0.12 g/L d-glucaric acid from 15 g/L Avicel and 0.45 ± 0.06 g/L d-glucaric acid from 15 g/L steam-exploded corn stover (SECS), respectively. In an attempt to design the microbial consortium for more efficient CBP, the team consisting of T. reesei Rut-C30 and S. cerevisiae LGA-1 was found to be the best, with excellent work distribution and collaboration. Conclusions Two engineered S. cerevisiae strains, LGA-1 and LGA-C, with high titers of d-glucaric acid were obtained. This indicated that S. cerevisiae INVSc1 is an excellent host for d-glucaric acid production. Lignocellulose is a preferable substrate over myo-inositol. SHF, SSF, and CBP were studied, and CBP using an artificial microbial consortium of T. reesei Rut-C30 and S. cerevisiae LGA-1 was found to be promising because of its relatively high titer and yield. T. reesei Rut-C30 and S. cerevisiae LGA-1were proven to be the best teammates for CBP. Further work should be done to improve the efficiency of this microbial consortium for d-glucaric acid production from lignocellulose.

scholarly journals Effect of Cellulase-producing Microbial Consortium on Biogas Production from Lignocellulosic Biomass

2017 ◽  
Vol 141 ◽  
pp. 180-183 ◽  
Author(s):  
Prapakorn Tantayotai ◽  
Peerapong Pornwongthong ◽  
Chotika Muenmuang ◽  
Theerawut Phusantisampan ◽  
Malinee Sriariyanun
Keyword(s):  
Lignocellulosic Biomass ◽  
Microbial Consortium ◽  
Biogas Production

scholarly journals The Role of Yeast-Surface-Display Techniques in Creating Biocatalysts for Consolidated BioProcessing

Catalysts ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 94 ◽  
Author(s):  
Ian Dominic Flormata Tabañag ◽  
I-Ming Chu ◽  
Yu-Hong Wei ◽  
Shen-Long Tsai
Keyword(s):  
Climate Change ◽  
Lignocellulosic Biomass ◽  
Surface Engineering ◽  
Consolidated Bioprocessing ◽  
Surface Display ◽  
Yeast Surface Display ◽  
Qualitative Assessment ◽  
The Status ◽  
Yeast Surface

Climate change is directly linked to the rapid depletion of our non-renewable fossil resources and has posed concerns on sustainability. Thus, imploring the need for us to shift from our fossil based economy to a sustainable bioeconomy centered on biomass utilization. The efficient bioconversion of lignocellulosic biomass (an ideal feedstock) to a platform chemical, such as bioethanol, can be achieved via the consolidated bioprocessing technology, termed yeast surface engineering, to produce yeasts that are capable of this feat. This approach has various strategies that involve the display of enzymes on the surface of yeast to degrade the lignocellulosic biomass, then metabolically convert the degraded sugars directly into ethanol, thus elevating the status of yeast from an immobilization material to a whole-cell biocatalyst. The performance of the engineered strains developed from these strategies are presented, visualized, and compared in this article to highlight the role of this technology in moving forward to our quest against climate change. Furthermore, the qualitative assessment synthesized in this work can serve as a reference material on addressing the areas of improvement of the field and on assessing the capability and potential of the different yeast surface display strategies on the efficient degradation, utilization, and ethanol production from lignocellulosic biomass.

scholarly journals Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization

2019 ◽  
Vol 13 (2) ◽  
pp. 410-422 ◽  
Author(s):  
Zhiqiang Wen ◽  
Qi Li ◽  
Jinle Liu ◽  
Mingjie Jin ◽  
Sheng Yang
Keyword(s):  
Consolidated Bioprocessing ◽  
Butanol Production

Microbial co-culturing systems: butanol production from organic wastes through consolidated bioprocessing

2018 ◽  
Vol 102 (13) ◽  
pp. 5419-5425 ◽  
Author(s):  
Yujia Jiang ◽  
Ting Zhang ◽  
Jiasheng Lu ◽  
Peter Dürre ◽  
Wenming Zhang ◽  
...  
Keyword(s):  
Consolidated Bioprocessing ◽  
Organic Wastes ◽  
Butanol Production

scholarly journals Strategies for improved isopropanol–butanol production by a Clostridium strain from glucose and hemicellulose through consolidated bioprocessing

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Fengxue Xin ◽  
Tianpeng Chen ◽  
Yujiang Jiang ◽  
Weiliang Dong ◽  
Wenming Zhang ◽  
...  
Keyword(s):  
Consolidated Bioprocessing ◽  
Butanol Production
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