Amelioration of Diabetes in a Murine Model upon Transplantation of Pancreatic β-Cells with Optogenetic Control of Cyclic Adenosine Monophosphate

2019 ◽  
Vol 8 (10) ◽  
pp. 2248-2255
Author(s):  
Fan Zhang ◽  
Emmanuel S. Tzanakakis
Keyword(s):  
Murine Model ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Optogenetic Control

Bile acid-polymer-probucol microparticles: protective effect on pancreatic β-cells and decrease in type 1 diabetes development in a murine model

2019 ◽  
Vol 24 (10) ◽  
pp. 1272-1277 ◽  
Author(s):  
Armin Mooranian ◽  
Nassim Zamani ◽  
Giuseppe Luna ◽  
Hesham Al-Sallami ◽  
Momir Mikov ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Bile Acid ◽  
Protective Effect ◽  
Murine Model ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Diabetes Development

scholarly journals Spatially compartmentalized phase regulation of a Ca2+-cAMP-PKA oscillatory circuit

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Brian Tenner ◽  
Michael Getz ◽  
Brian Ross ◽  
Donya Ohadi ◽  
Christopher H Bohrer ◽  
...  
Keyword(s):  
Relative Phase ◽  
Phase Relationship ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Signaling Networks ◽  
Adenylyl Cyclases ◽  
Β Cells ◽  
Oscillatory Circuit ◽  
Spatiotemporal Regulation ◽  
High Degree

Signaling networks are spatiotemporally organized to sense diverse inputs, process information, and carry out specific cellular tasks. In β cells, Ca2+, cyclic adenosine monophosphate (cAMP), and Protein Kinase A (PKA) exist in an oscillatory circuit characterized by a high degree of feedback. Here, we describe a mode of regulation within this circuit involving a spatial dependence of the relative phase between cAMP, PKA, and Ca2+. We show that in mouse MIN6 β cells, nanodomain clustering of Ca2+-sensitive adenylyl cyclases (ACs) drives oscillations of local cAMP levels to be precisely in-phase with Ca2+ oscillations, whereas Ca2+-sensitive phosphodiesterases maintain out-of-phase oscillations outside of the nanodomain. Disruption of this precise phase relationship perturbs Ca2+ oscillations, suggesting the relative phase within an oscillatory circuit can encode specific functional information. This work unveils a novel mechanism of cAMP compartmentation utilized for localized tuning of an oscillatory circuit and has broad implications for the spatiotemporal regulation of signaling networks.

ISLET CONTENTS OF CYCLIC 3′,5′-GUANOSINE MONOPHOSPHATE UNDER CONDITIONS WHICH AFFECT THE CYCLIC 3′,5′-ADENOSINE MONOPHOSPHATE

1977 ◽  
Vol 86 (2) ◽  
pp. 344-354 ◽  
Author(s):  
Eva Gagerman ◽  
Bo Hellman
Keyword(s):  
Turnover Rate ◽  
Phosphodiesterase Inhibitor ◽  
Dry Weight ◽  
Adenosine Monophosphate ◽  
Guanosine Monophosphate ◽  
Simple Pattern ◽  
Β Cells ◽  
Β Cell ◽  
Pancreatic Β Cells ◽  
High Concentrations

ABSTRACT The sensitivity of the radioimmunoassay for cGMP was considerably increased by previous 2′-O-succinylation of the nucleotide. The basal content of cGMP in β-cell-rich pancreatic islets isolated from ob/ob-mice was similar to that of cAMP, i. e. about 3 μmoles per kg dry weight. Extracellular Ca2+ was a prerequisite for maintaining this amount of cGMP. The islet cGMP differed from cAMP in being only slightly enhanced or not affected at all when the islets were exposed to high concentrations of glucose, the sulphydryl reagents chloromercuribenzene-p-sulphonic acid and iodoacetamide, or the potent phosphodiesterase inhibitor 3-isobutyl-l-methylxanthine. The data obtained suggest that the turnover rate for cGMP is much slower than that for cAMP in the pancreatic β-cells. The interrelationships between the two cyclic nucleotides do not seem to fit into a simple pattern of antagonism.

scholarly journals Optogenetic control of insulin secretion by pancreatic β-cells in vitro and in vivo

Gene Therapy ◽  
2015 ◽  
Vol 22 (7) ◽  
pp. 553-559 ◽  
Author(s):  
T Kushibiki ◽  
S Okawa ◽  
T Hirasawa ◽  
M Ishihara
Keyword(s):  
Insulin Secretion ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Optogenetic Control

HUMAN FOETAL PANCREATIC INSULIN SECRETION IN RESPONSE TO IONIC AND OTHER STIMULI

1971 ◽  
Vol 51 (2) ◽  
pp. 323-332 ◽  
Author(s):  
R. D. G. MILNER ◽  
A. J. BARSON ◽  
M. A. ASHWORTH
Keyword(s):  
Insulin Secretion ◽  
Insulin Release ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Β Cells ◽  
Pancreatic Insulin ◽  
Foetal Life ◽  
Acinar Tissue ◽  
Foetal Pancreas

SUMMARY Pieces of human foetal pancreas were incubated under control conditions and in media containing different stimuli of insulin release. Insulin secretion was stimulated from the pancreases of foetuses (83–625 g body weight) which were of 16–24 weeks gestational age. Potassium (60 mmol/l), barium (2·54 mmol/l) and ouabain (10−5 mol/l) were effective stimuli in all experiments. Glucagon (5 μg/ml), theophylline (1 mmol/l) and dibutyryl 3′,5′-cyclic adenosine monophosphate (1 mmol/l) stimulated insulin secretion in media containing 0, 0·6 or 3·0 mg glucose/ml. Theophylline and dibutyryl 3′,5′-cyclic adenosine monophosphate were effective in all experients and glucagon stimulated insulin release in four out of six experiments. At all ages studied, histological examination of the pancreas after each experiment revealed islets of Langerhans containing β cells. In most cases the islets were of the mantle type but occasionally bipolar islets were seen. Cellular normality, as judged by light microscopy, was preserved after periods of incubation for up to 5½ h. Glycogen was demonstrable in the pancreatic acinar tissue but not in the islets. The results of these experiments indicate that, between the 16th and 24th week of foetal life, the human β cell is capable of releasing insulin in vitro when stimulated appropriately.

Sign in / Sign up

Export Citation Format

Share Document