scholarly journals Spatiotemporal Dynamics of Synthetic Microbial Consortia in Microfluidic Devices

2019 ◽  
Vol 8 (9) ◽  
pp. 2051-2058 ◽  
Author(s):  
Razan N. Alnahhas ◽  
James J. Winkle ◽  
Andrew J. Hirning ◽  
Bhargav Karamched ◽  
William Ott ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Spatiotemporal Dynamics ◽  
Microbial Consortia ◽  
Synthetic Microbial Consortia

scholarly journals Spatiotemporal dynamics of synthetic microbial consortia in microfluidic devices

2019 ◽  
Author(s):  
Razan N. Alnahhas ◽  
James J. Winkle ◽  
Andrew J. Hirning ◽  
Bhargav Karamched ◽  
William Ott ◽  
...  
Keyword(s):  
Temporal Dynamics ◽  
Relative Proportion ◽  
Microfluidic Devices ◽  
Spatiotemporal Dynamics ◽  
Microbial Consortia ◽  
Intercellular Signaling ◽  
Single Strain ◽  
Trap Size ◽  
Different Strains ◽  
Synthetic Microbial Consortia

AbstractSynthetic microbial consortia consist of two or more engineered strains that grow together and share the same resources. When intercellular signaling pathways are included in the engineered strains, close proximity of the microbes can generate complex dynamic behaviors that are difficult to obtain using a single strain. However, when a consortium is not cultured in a well-mixed environment the constituent strains passively compete for space as they grow and divide, complicating cell-cell signaling. Here, we explore the temporal dynamics of the spatial distribution of consortia co-cultured in microfluidic devices. To do this, we grew two different strains ofEscherichia coliin microfluidic devices with cell-trapping regions (traps) of several different designs. We found that the size and shape of the traps are critical determinants of spatiotemporal dynamics. In small traps, cells can easily signal one another but the relative proportion of each strain within the trap can fluctuate wildly. In large traps, the relative ratio of strains is stabilized, but intercellular signaling can be hindered by distances between cells. This presents a trade-off between the trap size and the effectiveness of intercellular signaling, which can be mitigated by controlling the initial seeding of cells in the large trap. These results show how synthetic microbial consortia behave in microfluidic traps and provide a method to help remedy the spatial heterogeneity inherent to different trap geometries.

Characterization of a designed synthetic autotrophic–heterotrophic consortia for fixing CO2 without light

RSC Advances ◽  
2016 ◽  
Vol 6 (81) ◽  
pp. 78161-78169 ◽  
Author(s):  
Jiajun Hu ◽  
Yiyun Xue ◽  
Jixiang Li ◽  
Lei Wang ◽  
Shiping Zhang ◽  
...  
Keyword(s):  
Microbial Interactions ◽  
Microbial Consortia ◽  
Synthetic Microbial Consortia

CO2 fixation efficiency of the devised synthetic microbial consortia with both autotrophic–autotrophic and autotrophic–heterotrophic microbial interactions were higher than the sum of theoretical CO2 fixation efficiency of the microbial components.

scholarly journals Majority sensing in synthetic microbial consortia

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Razan N. Alnahhas ◽  
Mehdi Sadeghpour ◽  
Ye Chen ◽  
Alexis A. Frey ◽  
William Ott ◽  
...  
Keyword(s):  
Microbial Consortia ◽  
Synthetic Microbial Consortia

Design of synthetic microbial consortia for gut microbiota modulation

2019 ◽  
Vol 49 ◽  
pp. 52-59 ◽  
Author(s):  
Jorge F. Vázquez-Castellanos ◽  
Anaïs Biclot ◽  
Gino Vrancken ◽  
Geert RB Huys ◽  
Jeroen Raes
Keyword(s):  
Gut Microbiota ◽  
Microbial Consortia ◽  
Synthetic Microbial Consortia

Towards synthetic microbial consortia for bioprocessing

2012 ◽  
Vol 23 (5) ◽  
pp. 798-802 ◽  
Author(s):  
Jasmine Shong ◽  
Manuel Rafael Jimenez Diaz ◽  
Cynthia H Collins
Keyword(s):  
Microbial Consortia ◽  
Synthetic Microbial Consortia

scholarly journals Design, analysis and application of synthetic microbial consortia

2016 ◽  
Vol 1 (2) ◽  
pp. 109-117 ◽  
Author(s):  
Xiaoqiang Jia ◽  
Chang Liu ◽  
Hao Song ◽  
Mingzhu Ding ◽  
Jin Du ◽  
...  
Keyword(s):  
Design Analysis ◽  
Microbial Consortia ◽  
Synthetic Microbial Consortia

scholarly journals Tools for engineering coordinated system behaviour in synthetic microbial consortia

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Nicolas Kylilis ◽  
Zoltan A. Tuza ◽  
Guy-Bart Stan ◽  
Karen M. Polizzi
Keyword(s):  
Microbial Consortia ◽  
System Behaviour ◽  
Synthetic Microbial Consortia
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