Characterization of a designed synthetic autotrophic–heterotrophic consortia for fixing CO2 without light

RSC Advances ◽  
2016 ◽  
Vol 6 (81) ◽  
pp. 78161-78169 ◽  
Author(s):  
Jiajun Hu ◽  
Yiyun Xue ◽  
Jixiang Li ◽  
Lei Wang ◽  
Shiping Zhang ◽  
...  
Keyword(s):  
Microbial Interactions ◽  
Microbial Consortia ◽  
Synthetic Microbial Consortia

CO2 fixation efficiency of the devised synthetic microbial consortia with both autotrophic–autotrophic and autotrophic–heterotrophic microbial interactions were higher than the sum of theoretical CO2 fixation efficiency of the microbial components.

scholarly journals Costless metabolic secretions as drivers of interspecies interactions in microbial ecosystems

2018 ◽  
Author(s):  
Alan R. Pacheco ◽  
Mauricio Moel ◽  
Daniel Segrè
Keyword(s):  
Microbial Interactions ◽  
Fitness Cost ◽  
Microbial Consortia ◽  
Interspecies Interactions ◽  
Large Space ◽  
Microbial Ecosystems ◽  
Scale Models ◽  
Genome Scale ◽  
Synthetic Microbial Consortia ◽  
Metabolic Exchange

ABSTRACTMetabolic exchange can mediate beneficial interactions among microbes, helping explain diversity in microbial communities. These interactions are often assumed to involve a fitness cost, prompting questions on how cooperative phenotypes can be stable and withstand the emergence of cheaters. Here we use genome-scale models of metabolism to investigate whether a radically different scenario, the pervasive release of “costless” metabolites (i.e. those that cause no fitness cost to the producing organism), can serve as a prominent mechanism for inter-microbial interactions. By carrying out over 1 million pairwise growth simulations for 14 microbial species in a combinatorial assortment of environmental conditions, we find that there is indeed a large space of metabolites that can be secreted at no cost, which can generate ample cross-feeding opportunities. In addition to providing an atlas of putative costless interdependencies, our modeling also demonstrates that oxygen availability significantly enhances mutualistic interactions by providing more opportunities for metabolic exchange through costless metabolites, resulting in an over-representation of specific ecological network motifs. In addition to helping explain natural diversity, we show how the exchange of costless metabolites can facilitate the engineering of stable synthetic microbial consortia.

CHARACTERIZATION OF FORMATION WATERS IN THE PARADOX BASIN TO CONSTRAIN PALEOFLUID FLOW AND WATER-ROCK-MICROBIAL INTERACTIONS

2019 ◽  
Author(s):  
Jihyun Kim ◽  
◽  
Chandler Noyes ◽  
Ambria Dell'Oro ◽  
Rebecca Tyne ◽  
...  
Keyword(s):  
Microbial Interactions ◽  
Paradox Basin

scholarly journals Sequencing and Computational Approaches to Identification and Characterization of Microbial Organisms

2013 ◽  
Vol 5 ◽  
pp. BECB.S10886 ◽  
Author(s):  
Brijesh Singh Yadav ◽  
Venkateswarlu Ronda ◽  
Dinesh P. Vashista ◽  
Bhaskar Sharma
Keyword(s):  
Sequence Data ◽  
Microbial Interactions ◽  
Microbial Pathogens ◽  
Nucleotide Sequence Data ◽  
Computational Approaches ◽  
Microbial Detection ◽  
Sequencing Technologies ◽  
Sequencing Platforms ◽  
Identification And Characterization

The recent advances in sequencing technologies and computational approaches are propelling scientists ever closer towards complete understanding of human-microbial interactions. The powerful sequencing platforms are rapidly producing huge amounts of nucleotide sequence data which are compiled into huge databases. This sequence data can be retrieved, assembled, and analyzed for identification of microbial pathogens and diagnosis of diseases. In this article, we present a commentary on how the metagenomics incorporated with microarray and new sequencing techniques are helping microbial detection and characterization.

scholarly journals The Enrichment And Characterization Of Compost And Wastewater-Derived Microbial Cellulolytic Consortia For Biofuel Production

2021 ◽  
Author(s):  
Augustyna Dobosz
Keyword(s):  
Energy Demand ◽  
Defined Medium ◽  
Biofuel Production ◽  
Microbial Consortia ◽  
Extraction And Purification ◽  
End Products ◽  
Fermentative Activity ◽  
Incubation Temperatures ◽  
Current Energy

Over the last decade, a rise in energy demand and diminishing fuel resources have created a challenge for finding an alternative solution that could supplement our current energy sources. This study demonstrated that ethanol and other useful end-products can be produced from the fermentative activity of microbial consortia derived from cellulose-rich waste environments. Compost and wastewater were used as inoculum sources to enrich cellulolytic cultures at incubation temperatures 50 ºC and 60ºC. A chemically defined medium was used without complex nutrients such as yeast extract. Four cellulolytic cultures were obtained and their end-products were monitored over an active cellulose degrading period. The compost culture incubated at 50ºC produced the highest concentration of butyrate while the wastewater-derived culture incubated at 60ºC produced the highest ethanol concentration. Optimization of DNA extraction and purification from complex environmental samples such as the compost and wastewater cultures used in this study was also discussed.

scholarly journals The extracellular contractile injection system is enriched in environmental microbes and associates with numerous toxins

2020 ◽  
Author(s):  
Alexander Martin Geller ◽  
Inbal Pollin ◽  
David Zlotkin ◽  
Aleks Danov ◽  
Nimrod Nachmias ◽  
...  
Keyword(s):  
Control Systems ◽  
Protein Delivery ◽  
Microbial Interactions ◽  
Host Cells ◽  
Injection System ◽  
Prokaryotic Genomes ◽  
Immunity Genes ◽  
Core Genes ◽  
Environmental Microbes

AbstractBacteria employ toxin delivery systems to exclude bacterial competitors and to infect host cells. Characterization of these systems and the toxins they secrete is important for understanding microbial interactions and virulence in different ecosystems. The extracellular Contractile Injection System (eCIS) is a toxin delivery particle that evolved from a bacteriophage tail. Four known eCIS systems have been shown to mediate interactions between bacteria and their invertebrate hosts, but the broad ecological function of these systems remains unknown. Here, we identify eCIS loci in 1,249 prokaryotic genomes and reveal a striking enrichment of these loci in environmental microbes and absence from mammalian pathogens. We uncovered 13 toxin genes that associate with eCIS from diverse microbes and show that they can inhibit growth of bacteria, yeast or both. We also found immunity genes that protect bacteria from self-intoxication, supporting an antibacterial role for eCIS. Furthermore, we identified multiple new eCIS core genes including a conserved eCIS transcriptional regulator. Finally, we present our data through eCIStem; an extensive eCIS repository. Our findings define eCIS as a widespread environmental prokaryotic toxin delivery system that likely mediates antagonistic interactions with eukaryotes and prokaryotes. Future understanding of eCIS functions can be leveraged for the development of new biological control systems, antimicrobials, and cell-free protein delivery tools.

scholarly journals Majority sensing in synthetic microbial consortia

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Razan N. Alnahhas ◽  
Mehdi Sadeghpour ◽  
Ye Chen ◽  
Alexis A. Frey ◽  
William Ott ◽  
...  
Keyword(s):  
Microbial Consortia ◽  
Synthetic Microbial Consortia
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