scholarly journals Combining 26s rDNA and the Cre-loxP System for Iterative Gene Integration and Efficient Marker Curation in Yarrowia lipolytica

2019 ◽  
Vol 8 (3) ◽  
pp. 568-576 ◽  
Author(s):  
Yongkun Lv ◽  
Harley Edwards ◽  
Jingwen Zhou ◽  
Peng Xu
Keyword(s):  
Yarrowia Lipolytica ◽  
26S Rdna ◽  
Gene Integration

scholarly journals A novel and effective Cre/lox-based genetic tool for repeated, targeted and markerless integration in Yarrowia lipolytica

2020 ◽  
Author(s):  
Qinghua Zhou ◽  
Liangcheng Jiao ◽  
Wenjuan Li ◽  
Zhiming Hu ◽  
Yunchong Li ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Genome Engineering ◽  
Selection Marker ◽  
The Novel ◽  
Genetic Tool ◽  
Recombination System ◽  
Marker Free ◽  
Free Gene ◽  
Gene Integration

AbstractThe unconventional yeast Yarrowia lipolytica is extensively applied in bioproduction fields owing to its excellent metabolite and protein production ability. Nonetheless, utilization of this promising host is still restricted by limited availability of precise and effective gene integration tools. In this study, a novel and efficient genetic tool was developed for targeted, repeated, and markerless gene integration based on Cre/lox sitespecific recombination system. The developed tool required only a single selection marker and could completely excise all of the unnecessary sequences. A total of three plasmids were created and seven rounds of marker-free gene integration were examined in Y. lipolytica. All the integration efficiencies remained above 90%, and analysis of protein production and growth characteristics of the engineered strains confirmed that genome modification via the novel genetic tool was feasible. Further work also confirmed the genetic tool was effective for integration of other genes, loci, and strains. Thus, this study significantly promotes the application of Cre/lox system and presents a powerful tool for genome engineering in Y. lipolytica.

Multiple gene integration to promote erythritol production on glycerol in Yarrowia lipolytica

2021 ◽  
Author(s):  
Ling Zhang ◽  
Ming-Yue Nie ◽  
Feng Liu ◽  
Jun Chen ◽  
Liu-Jing Wei ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Multiple Gene ◽  
Gene Integration

scholarly journals A Novel Cre/lox-Based Genetic Tool for Repeated, Targeted and Markerless Gene Integration in Yarrowia lipolytica

2021 ◽  
Vol 22 (19) ◽  
pp. 10739
Author(s):  
Qinghua Zhou ◽  
Liangcheng Jiao ◽  
Wenjuan Li ◽  
Zhiming Hu ◽  
Yunchong Li ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Genome Engineering ◽  
Selection Marker ◽  
The Novel ◽  
Genetic Tool ◽  
Recombination System ◽  
Marker Free ◽  
Free Gene ◽  
Gene Integration

The unconventional yeast Yarrowia lipolytica is extensively applied in bioproduction fields owing to its excellent metabolite and protein production ability. Nonetheless, utilization of this promising host is still restricted by the limited availability of precise and effective gene integration tools. In this study, a novel and efficient genetic tool was developed for targeted, repeated, and markerless gene integration based on Cre/lox site-specific recombination system. The developed tool required only a single selection marker and could completely excise the unnecessary sequences. A total of three plasmids were created and seven rounds of marker-free gene integration were examined in Y. lipolytica. All the integration efficiencies remained above 90%, and analysis of the protein production and growth characteristics of the engineered strains confirmed that genome modification via the novel genetic tool was feasible. Further work also confirmed that the genetic tool was effective for the integration of other genes, loci, and strains. Thus, this study significantly promotes the application of the Cre/lox system and presents a powerful tool for genome engineering in Y. lipolytica.

Effect of essential oil of Citrus cinensis cv new hall – Citrus aurantium (indigenous in Greece) upon growth of Yarrowia lipolytica

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
O Gortzi ◽  
S Papanikolaou ◽  
S Lalas ◽  
M Galiotou-Panayotou ◽  
P Mitliaga
Keyword(s):  
Essential Oil ◽  
Yarrowia Lipolytica ◽  
Citrus Aurantium

Effect of Yarrowia lipolytica RO25 cricket-based hydrolysates on sourdough quality parameters

LWT ◽  
2021 ◽  
pp. 111760
Author(s):  
Samantha Rossi ◽  
Luigi Parrotta ◽  
Stefano Del Duca ◽  
Marco Dalla Rosa ◽  
Francesca Patrignani ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Quality Parameters

scholarly journals Aeration and Stirring in Yarrowia lipolytica Lipase Biosynthesis during Batch Cultures with Waste Fish Oil as a Carbon Source

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 88
Author(s):  
Paulina Snopek ◽  
Dorota Nowak ◽  
Bartłomiej Zieniuk ◽  
Agata Fabiszewska
Keyword(s):  
Carbon Source ◽  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Lipolytic Activity ◽  
Culture Conditions ◽  
Agitation Speed ◽  
Oxygen Deficit ◽  
Shear Stresses ◽  
Process Selection ◽  
Batch Cultures

Yarrowia lipolytica is one of the most studied non-conventional forms of yeast, exhibiting a high secretory capacity and producing many industrially important and valuable metabolites. The yeast conceals a great biotechnological potential to synthesize organic acids, sweeteners, microbial oil, or fragrances. The vast majority of bioprocesses are carried out in bioreactors, where suitable culture conditions are provided. In the current study, the effect of agitation speed (200–600 rpm) and air flow rate (0.0375–2.0 dm3/(dm3 × min)) on the biomass yield and lipase activity of Y. lipolytica KKP 379 is analyzed in a growth medium containing waste fish oil. The increase of aeration intensity limited the period of oxygen deficit in the medium. Simultaneously, an increase in lipolytic activity was observed from 2.09 U/cm3 to 14.21 U/cm3; however, an excessive agitation speed likely caused oxidative or shear stresses, and a reduction in lipolytic activity was observed. Moreover, it is confirmed that the synthesis of lipases is related to oxygen consumption, pH, and the yeast growth phase, and appropriate process selection may provide two advantages, namely, the maximum use of the waste carbon source and the production of lipolytic enzymes that are valuable in many industries.

Lipid bioproduction from delignified native grass (Cyperus distans) hydrolysate by Yarrowia lipolytica

2021 ◽  
pp. 124659
Author(s):  
Satish Kommoji ◽  
M. Gopinath ◽  
Polinati Satya Sagar ◽  
D. Yuvaraj ◽  
J. Iyyappan ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Native Grass
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