A Living Eukaryotic Autocementation Kit from Surface Display of Silica Binding Peptides on Yarrowia lipolytica

2016 ◽  
Vol 5 (12) ◽  
pp. 1466-1474 ◽  
Author(s):  
Shuyan Tang ◽  
Wang Xi ◽  
Zhangyu Cheng ◽  
Lei Yin ◽  
Ruihao Li ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Surface Display ◽  
Binding Peptides

The Surface Display of the Alginate Lyase on the Cells of Yarrowia lipolytica for Hydrolysis of Alginate

2009 ◽  
Vol 11 (5) ◽  
pp. 619-626 ◽  
Author(s):  
Guanglei Liu ◽  
Lixi Yue ◽  
Zhe Chi ◽  
Wengong Yu ◽  
Zhenming Chi ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Surface Display ◽  
Alginate Lyase ◽  
Hydrolysis Of

Surface display of acid protease on the cells of Yarrowia lipolytica for milk clotting

2010 ◽  
Vol 87 (2) ◽  
pp. 669-677 ◽  
Author(s):  
Xin-Jun Yu ◽  
Catherine Madzak ◽  
Hui-Juan Li ◽  
Zhen-Ming Chi ◽  
Jing Li
Keyword(s):  
Yarrowia Lipolytica ◽  
Surface Display ◽  
Acid Protease ◽  
Milk Clotting

Efficient cell surface display of Lip2 lipase using C-domains of glycosylphosphatidylinositol-anchored cell wall proteins of Yarrowia lipolytica

2011 ◽  
Vol 91 (3) ◽  
pp. 645-654 ◽  
Author(s):  
Evgeniya Y. Yuzbasheva ◽  
Tigran V. Yuzbashev ◽  
Ivan A. Laptev ◽  
Tatiana K. Konstantinova ◽  
Sergey P. Sineoky
Keyword(s):  
Cell Wall ◽  
Cell Surface ◽  
Yarrowia Lipolytica ◽  
Surface Display ◽  
Cell Surface Display ◽  
Cell Wall Proteins ◽  
Lip2 Lipase

scholarly journals Antibody-guided design and identification of CD25-binding small antibody mimetics using mammalian cell surface display

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kyra See ◽  
Tetsuya Kadonosono ◽  
Kotaro Miyamoto ◽  
Takuya Tsubaki ◽  
Yumi Ota ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Dissociation Constants ◽  
Surface Display ◽  
Interleukin 2 ◽  
Peptide Identification ◽  
Cell Surface Display ◽  
Affinity Maturation ◽  
High Affinity ◽  
Binding Peptides ◽  
Target Binding

AbstractSmall antibody mimetics that contain high-affinity target-binding peptides can be lower cost alternatives to monoclonal antibodies (mAbs). We have recently developed a method to create small antibody mimetics called FLuctuation-regulated Affinity Proteins (FLAPs), which consist of a small protein scaffold with a structurally immobilized target-binding peptide. In this study, to further develop this method, we established a novel screening system for FLAPs called monoclonal antibody-guided peptide identification and engineering (MAGPIE), in which a mAb guides selection in two manners. First, antibody-guided design allows construction of a peptide library that is relatively small in size, but sufficient to identify high-affinity binders in a single selection round. Second, in antibody-guided screening, the fluorescently labeled mAb is used to select mammalian cells that display FLAP candidates with high affinity for the target using fluorescence-activated cell sorting. We demonstrate the reliability and efficacy of MAGPIE using daclizumab, a mAb against human interleukin-2 receptor alpha chain (CD25). Three FLAPs identified by MAGPIE bound CD25 with dissociation constants of approximately 30 nM as measured by biolayer interferometry without undergoing affinity maturation. MAGPIE can be broadly adapted to any mAb to develop small antibody mimetics.

scholarly journals Directed Evolution and Engineering of Gallium-Binding Phage Clones—A Preliminary Study

Biomimetics ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 35 ◽  
Author(s):  
Nora Schönberger ◽  
Christina Zeitler ◽  
Robert Braun ◽  
Franziska L. Lederer ◽  
Sabine Matys ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Metal Binding ◽  
Surface Display ◽  
Protein Structures ◽  
Amino Acid Position ◽  
Site Directed Mutagenesis ◽  
Peptide Sequence ◽  
Peptide Structure ◽  
Cysteine Scanning ◽  
Binding Peptides

The phage surface display technology is a useful tool to screen and to extend the spectrum of metal-binding protein structures provided by nature. The directed evolution approach allows identifying specific peptide ligands for metals that are less abundant in the biosphere. Such peptides are attractive molecules in resource technology. For example, gallium-binding peptides could be applied to recover gallium from low concentrated industrial wastewater. In this study, we investigated the affinity and selectivity of five bacteriophage clones displaying different gallium-binding peptides towards gallium and arsenic in independent biosorption experiments. The displayed peptides were highly selective towards Ga3+ whereby long linear peptides showed a lower affinity and specificity than those with a more rigid structure. Cysteine scanning was performed to determine the relationship between secondary peptide structure and gallium sorption. By site-directed mutagenesis, the amino acids of a preselected peptide sequence are systematically replaced by cysteines. The resulting disulphide bridge considerably reduces the flexibility of linear peptides. Subsequent biosorption experiments carried out with the mutants obtained from cysteine scanning demonstrated, depending on the position of the cysteines in the peptide, either a considerable increase in the affinity of gallium compared to arsenic or an increase in the affinity for arsenic compared to gallium. This study shows the impressive effect on peptide–target interaction based on peptide structure and amino acid position and composition via the newly established systematic cysteine scanning approach.

Bacterial surface display of metal binding peptides as whole-cell biocatalysts for 4-nitroaniline reduction

RSC Advances ◽  
2015 ◽  
Vol 5 (107) ◽  
pp. 87998-88001 ◽  
Author(s):  
Dong-Yu Tsai ◽  
Yi-Jung Tsai ◽  
Chia-Ho Yen ◽  
Chun-Yu Ouyang ◽  
Yi-Chun Yeh
Keyword(s):  
Metal Binding ◽  
Surface Display ◽  
Whole Cell ◽  
Bacterial Surface ◽  
Bacterial Surface Display ◽  
Metal Binding Peptides ◽  
Recombinant Microorganisms ◽  
Binding Peptides

Using recombinant microorganisms expressing metal binding peptides as whole-cell biocatalysts for 4-nitroaniline reduction.

Development of Metal Ion Binding Peptides Using Phage Surface Display Technology

2017 ◽  
Vol 262 ◽  
pp. 591-595 ◽  
Author(s):  
Nora Schönberger ◽  
Sabine Matys ◽  
Katrin Flemming ◽  
Falk Lehmann ◽  
Franziska L. Lederer ◽  
...  
Keyword(s):  
Metal Ion ◽  
Specific Binding ◽  
Surface Display ◽  
Ionic Species ◽  
Phage Display Library ◽  
Complexing Agents ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Display Technology ◽  
Binding Peptides

Phage surface display technology is a useful tool for the identification of biosorptive peptides. In this work it is used for the identification of cobalt, nickel and gallium binding peptides. We present methods for the enrichment of metal ion binding bacteriophage clones from a commercial phage display library. Metal ion selective peptides are suitable to separate as well as concentrate cobalt and nickel from copper black shale leaching products (EcoMetals project) and gallium from industrial waste waters (EcoGaIn project). In contrast to common capture methods of specific binding phage for solid materials the ionic species have to be immobilized prior to the bio-panning procedure. This was realized by chemical complexation of the metal ions using commercial complexing agents on porous matrices. Moreover, an option to harvest non elutable strong binding phage is proposed.

Construction of a new plasmid for surface display on cells of Yarrowia lipolytica

2008 ◽  
Vol 72 (2) ◽  
pp. 116-123 ◽  
Author(s):  
Lixi Yue ◽  
Zhenming Chi ◽  
Lin Wang ◽  
Jia Liu ◽  
Catherine Madzak ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Surface Display
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