scholarly journals Engineering Isoprenoid Quinone Production in Yeast

2020 ◽  
Vol 9 (9) ◽  
pp. 2239-2245 ◽  
Author(s):  
Divjot Kaur ◽  
Duha Alkhder ◽  
Christophe Corre ◽  
Fabrizio Alberti
Keyword(s):  
Isoprenoid Quinone

scholarly journals Engineering isoprenoid quinone production in yeast

2020 ◽  
Author(s):  
Divjot Kaur ◽  
Christophe Corre ◽  
Fabrizio Alberti
Keyword(s):  
Yeast Strain ◽  
Mevalonate Pathway ◽  
Coenzyme Q ◽  
Shikimate Pathway ◽  
Bioactive Molecules ◽  
Primary Metabolism ◽  
Isoprenoid Quinone ◽  
New Host ◽  
Domains Of Life ◽  
Isoprenoid Quinones

Isoprenoid quinones are bioactive molecules that include an isoprenoid chain and a quinone head. They are traditionally found to be involved in primary metabolism, where they act as electron transporters, but specialized isoprenoid quinones are also produced by all domains of life. Here, we report the engineering of a baker's yeast strain, Saccharomyces cerevisiae EPYFA3, for the production of isoprenoid quinones. Our yeast strain was developed through overexpression of the shikimate pathway in a well-established recipient strain (S. cerevisiae EPY300) where the mevalonate pathway is overexpressed. As a proof of concept, our new host strain was used to overproduce the endogenous isoprenoid quinone coenzyme Q6, resulting in a final four-fold production increase. EPYFA3 represents a valuable platform for the heterologous production of high value isoprenoid quinones. EPYFA3 will also facilitate the elucidation of isoprenoid quinone biosynthetic pathways.

A note on the isoprenoid quinone content ofBordetella aviumand related species

1987 ◽  
Vol 62 (3) ◽  
pp. 275-277 ◽  
Author(s):  
Mary T. Fletcher ◽  
P. J. Blackall ◽  
Catherine M. Doheny
Keyword(s):  
Related Species ◽  
Isoprenoid Quinone

scholarly journals Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain

2005 ◽  
Vol 55 (6) ◽  
pp. 2385-2390 ◽  
Author(s):  
Fernando Martínez-Checa ◽  
Victoria Béjar ◽  
Inmaculada Llamas ◽  
Ana del Moral ◽  
Emilia Quesada
Keyword(s):  
Fatty Acid ◽  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Halophilic Bacterium ◽  
Southern Spain ◽  
Isoprenoid Quinone ◽  
Hypersaline Water ◽  
Phenotypic Tests ◽  
Major Isoprenoid Quinone

Strain F-32T, which produces exopolysaccharides and contains polyunsaturated fatty acids, was isolated from a hypersaline water sample collected from Fuente de Piedra (southern Spain). Phylogenetic analyses indicated conclusively that the strain in question belonged to the genus Alteromonas. Phenotypic tests showed that it could be assigned to the genus Alteromonas although it had a number of distinctive characteristics: it is moderately halophilic, growing best with 7·5–10 % w/v NaCl; it grows at 4 °C and produces H2S; it does not grow with d-cellobiose, d-fructose, d-galactose, d-glucose or lactose as sole sources of carbon and energy; and its fatty-acid profile is typical of Alteromonas but it also contains a large amount of an unusual acid with three double bonds [18 : 3ω6c (6, 9, 12); 5·01 %, w/v]. The major isoprenoid quinone is Q8. The DNA G+C content is 46·3 mol%. The phylogenetic, phenotypic and genetic properties of strain F-32T place it within a novel species, for which the name Alteromonas hispanica sp. nov. is proposed. The type strain is F-32T (=CECT 7067T=LMG 22958T).

scholarly journals Exiguobacterium Sp. HA2, isolated from the Ilam Mountains of Iran

2021 ◽  
Author(s):  
Reza Heidari ◽  
Mostafa Akbariqomi ◽  
Gholamreza Tavoosidana ◽  
Garshasb Rigi
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Novel Species ◽  
Isoprenoid Quinone ◽  
Gram Stain ◽  
Phenotypic Characteristics ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Peptidoglycan Layer ◽  
Major Isoprenoid Quinone

ABSTRACTA motile, Gram-stain-positive, rod-shaped, non-sporing, tolerate up to 5% NaCl, grew at 0–25 °C, designated Exiguobacterium sp. HA2 was isolated from the soil of the Ilam Mountains of Iran during October 2016. The major isoprenoid quinone is MK-7 and in the smaller amount are MK-6 and MK-8. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine. Major fatty acids (>10 %) are isoC13:0, isoC15:0 and C16:0. The bacterial cell wall peptidoglycan layer was lysine-glycine. The 16S rRNA sequence was analyzed at the phylogenetic levels. Also, A supplemental comparison was made between five other genes including csp, gyrB, hsp70, rpoB, and citC. According to the results of genotypic and phenotypic characteristics, the strain was categorized in the genus Exiguobacterium. This bacterium had the closest relation with Exiguobacterium undae, and thus was dubbed Exiguobacterium sp. HA2. The different in the Phenotypic, functional characteristics and phylogenetic indicated Exiguobacterium sp. HA2 can be regarded as representing considered a novel species within the genus Exiguobacterium.

scholarly journals Solimonas terrae sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1218-1222 ◽  
Author(s):  
Soo-Jin Kim ◽  
Ji-Young Moon ◽  
Hang-Yeon Weon ◽  
Jae-Hyung Ahn ◽  
Wen-Ming Chen ◽  
...  
Keyword(s):  
Type Strain ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Isoprenoid Quinone ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated strain KIS83-12T, was isolated from soil of Gaui island in the Taean region of South Korea. The strain grew at 15–33 °C (optimum, 28 °C), at pH 5.0–8.0 (optimum, pH 7.0). Growth did not occur in the presence of NaCl. The strain was catalase-negative and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that KIS83-12T was most closely related to Solimonas soli DCY12T (96.9 %), Solimonas variicoloris MN28T (96.5 %), Solimonas flava CW-KD 4T (96.5 %) and Solimonas aquatica NAA16T (96.0 %), and formed a robust phyletic lineage with members of the genus Solimonas . The main isoprenoid quinone was Q-8. Major polar lipids included phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acids present in large and moderate amounts (>5.0 %) were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0, C16 : 1ω5c, summed feature 2 (iso-C16 : 1 I and/or C14 : 0 3-OH) and C12 : 0. The DNA G+C content was 67.9 mol%. On the basis of the taxonomic data obtained in this study, KIS83-12T represents a novel species of the genus Solimonas , for which the name Solimonas terrae sp. nov. is proposed, with KIS83-12T ( = KACC 16967T = DSM 27281T) as the type strain.

scholarly journals Isoprenoid quinone and polar lipid composition ofLeptospiraceae

1987 ◽  
Vol 44 (2) ◽  
pp. 273-276
Author(s):  
T.M. Embley ◽  
J.V. Hookey ◽  
M.D. Collins
Keyword(s):  
Polar Lipid ◽  
Lipid Composition ◽  
Isoprenoid Quinone

scholarly journals Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter

2010 ◽  
Vol 60 (1) ◽  
pp. 134-139 ◽  
Author(s):  
Keun Sik Baik ◽  
Seong Chan Park ◽  
Eun Mi Kim ◽  
Chae Hong Lim ◽  
Chi Nam Seong
Keyword(s):  
Fatty Acids ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Isoprenoid Quinone ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Emended Description ◽  
Major Isoprenoid Quinone

A non-motile, rod-shaped bacterium, designated strain WPCB133T, was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-reaction-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The strain contained MK-7 as the major isoprenoid quinone. The DNA G+C content was 47 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB133T forms an independent lineage within the genus Mucilaginibacter. Strain WPCB133T was distantly related to Mucilaginibacter kameinonensis SCKT (94.7 % sequence similarity), Mucilaginibacter paludis TPT56T (94.5 %) and Mucilaginibacter gracilis TPT18T (94.4 %). Phenotypic characteristics distinguished strain WPCB133T from members of the genus Mucilaginibacter. On the basis of evidence presented in this study, strain WPCB133T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter rigui sp. nov. is proposed. The type strain is WPCB133T (=KCTC 12534T =NBRC 101115T). An emended description of the genus Mucilaginibacter is also proposed.

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