An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

ACS Sensors ◽  
2019 ◽  
Vol 4 (6) ◽  
pp. 1560-1568 ◽  
Author(s):  
Akkapol Suea-Ngam ◽  
Philip D. Howes ◽  
Claire E. Stanley ◽  
Andrew J. deMello
Keyword(s):  
Ochratoxin A ◽  
Electrochemical Aptasensor ◽  
Exonuclease I

Fabrication of electro-active nano-trans surfaces to design label free electrochemical aptasensor for ochratoxin A detection

2021 ◽  
Vol 379 ◽  
pp. 138172
Author(s):  
Maham Liaqat ◽  
Sara Riaz ◽  
Mian Hasnain Nawaz ◽  
Mihaela Badea ◽  
Akhtar Hayat ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Label Free ◽  
Electrochemical Aptasensor

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

BMC Chemistry ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0 × 10−3–1000 ng mL−1 with a low limit of detection of 0.15 pg mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

A novel electrochemical aptasensor based on arch-shape structure of aptamer-complimentary strand conjugate and exonuclease I for sensitive detection of streptomycin

2016 ◽  
Vol 75 ◽  
pp. 123-128 ◽  
Author(s):  
Noor Mohammad Danesh ◽  
Mohammad Ramezani ◽  
Ahmad Sarreshtehdar Emrani ◽  
Khalil Abnous ◽  
Seyed Mohammad Taghdisi
Keyword(s):  
Sensitive Detection ◽  
Electrochemical Aptasensor ◽  
Exonuclease I ◽  
Shape Structure ◽  
Arch Shape

An electrochemical aptasensor based on graphene doped chitosan nanocomposites for determination of Ochratoxin A

2019 ◽  
Vol 144 ◽  
pp. 102-109 ◽  
Author(s):  
Navpreet Kaur ◽  
Anu Bharti ◽  
Supriya Batra ◽  
Shilpa Rana ◽  
Shweta Rana ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Electrochemical Aptasensor

A novel electrochemical aptasensor for ochratoxin a sensing in spiked food using strand-displacement polymerase reaction

Talanta ◽  
2021 ◽  
Vol 223 ◽  
pp. 121705
Author(s):  
Seyed Mohammad Taghdisi ◽  
Noor Mohammad Danesh ◽  
Mohammad Ramezani ◽  
Mona Alibolandi ◽  
Morteza Alinezhad Nameghi ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Electrochemical Aptasensor ◽  
Strand Displacement

Ultrasensitive electrochemical aptasensor for ochratoxin A based on two-level cascaded signal amplification strategy

2014 ◽  
Vol 96 ◽  
pp. 7-13 ◽  
Author(s):  
Xingwang Yang ◽  
Jing Qian ◽  
Ling Jiang ◽  
Yuting Yan ◽  
Kan Wang ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Amplification Strategy

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

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