A novel electrochemical aptasensor based on arch-shape structure of aptamer-complimentary strand conjugate and exonuclease I for sensitive detection of streptomycin

2016 ◽  
Vol 75 ◽  
pp. 123-128 ◽  
Author(s):  
Noor Mohammad Danesh ◽  
Mohammad Ramezani ◽  
Ahmad Sarreshtehdar Emrani ◽  
Khalil Abnous ◽  
Seyed Mohammad Taghdisi
Keyword(s):  
Sensitive Detection ◽  
Electrochemical Aptasensor ◽  
Exonuclease I ◽  
Shape Structure ◽  
Arch Shape

A label-free electrochemical aptasensor based on the core–shell Cu-MOF@TpBD hybrid nanoarchitecture for the sensitive detection of PDGF-BB

The Analyst ◽  
2021 ◽  
Author(s):  
Ya Li ◽  
Zhiling Liu ◽  
Wenbo Lu ◽  
Man Zhao ◽  
He Xiao ◽  
...  
Keyword(s):  
Hybrid Nanocomposites ◽  
Sensitive Detection ◽  
Core Shell ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
The Core

A novel label-free electrochemical aptasensor based on core–shell Cu-MOF@TpBD hybrid nanocomposites has been prepared for the sensitive detection of PDGF-BB.

A regular “signal attenuation” electrochemical aptasensor for highly sensitive detection of streptomycin

2016 ◽  
Vol 40 (11) ◽  
pp. 9711-9718 ◽  
Author(s):  
Junling Yin ◽  
Wenjuan Guo ◽  
Xiaoli Qin ◽  
Meishan Pei ◽  
Luyan Wang ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Electrochemical Aptasensor ◽  
Signal Attenuation ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A novel aptasensor based on PCNRs and MWCNTs–CuO–AuNPs as the nanomatrix was constructed for the highly sensitive detection of streptomycin.

scholarly journals A Novel Sandwich-type Electrochemical Aptasensor Based on GR-3D Au and Aptamer-AuNPs-HRP for Sensitive Detection of Oxytetracycline

2017 ◽  
Vol 27 ◽  
pp. 151-152 ◽  
Author(s):  
Su Liu ◽  
Yu Wang ◽  
Wei Xu ◽  
Xueqi Leng ◽  
Hongzhi Wang ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Electrochemical Aptasensor ◽  
Sandwich Type

An Exonuclease I-Assisted Silver-Metallized Electrochemical Aptasensor for Ochratoxin A Detection

ACS Sensors ◽  
2019 ◽  
Vol 4 (6) ◽  
pp. 1560-1568 ◽  
Author(s):  
Akkapol Suea-Ngam ◽  
Philip D. Howes ◽  
Claire E. Stanley ◽  
Andrew J. deMello
Keyword(s):  
Ochratoxin A ◽  
Electrochemical Aptasensor ◽  
Exonuclease I

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

BMC Chemistry ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0 × 10−3–1000 ng mL−1 with a low limit of detection of 0.15 pg mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

Electrochemical aptasensor based on Au@HS-rGO and thymine-Hg2+-thymine structure for sensitive detection of mercury ion

2019 ◽  
Vol 848 ◽  
pp. 113308 ◽  
Author(s):  
Lei Zhao ◽  
Yaoguang Wang ◽  
Guanhui Zhao ◽  
Nuo Zhang ◽  
Yong Zhang ◽  
...  
Keyword(s):  
Mercury Ion ◽  
Sensitive Detection ◽  
Electrochemical Aptasensor
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