scholarly journals Enzyme-Assisted Nucleic Acid Detection for Infectious Disease Diagnostics: Moving toward the Point-of-Care

ACS Sensors ◽  
2020 ◽  
Vol 5 (9) ◽  
pp. 2701-2723
Author(s):  
Akkapol Suea-Ngam ◽  
Léonard Bezinge ◽  
Bogdan Mateescu ◽  
Philip D. Howes ◽  
Andrew J. deMello ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Nucleic Acid ◽  
Point Of Care ◽  
Nucleic Acid Detection ◽  
Disease Diagnostics ◽  
Infectious Disease Diagnostics

scholarly journals Advances in microfluidic PCR for point-of-care infectious disease diagnostics

2011 ◽  
Vol 29 (6) ◽  
pp. 830-839 ◽  
Author(s):  
Seungkyung Park ◽  
Yi Zhang ◽  
Shin Lin ◽  
Tza-Huei Wang ◽  
Samuel Yang
Keyword(s):  
Infectious Disease ◽  
Point Of Care ◽  
Disease Diagnostics ◽  
Infectious Disease Diagnostics

scholarly journals Paper microfluidics for nucleic acid amplification testing (NAAT) of infectious diseases

Lab on a Chip ◽  
2017 ◽  
Vol 17 (14) ◽  
pp. 2347-2371 ◽  
Author(s):  
Laura Magro ◽  
Camille Escadafal ◽  
Pierre Garneret ◽  
Béatrice Jacquelin ◽  
Aurélia Kwasiborski ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Infectious Diseases ◽  
Nucleic Acid Amplification ◽  
Paper Microfluidics ◽  
Nucleic Acid Amplification Testing ◽  
Disease Diagnostics ◽  
Infectious Disease Diagnostics

On-field infectious disease diagnostics can be performed with paper microfluidics through sample preparation and nucleic acid amplification.

scholarly journals Microneedle-based devices for point-of-care infectious disease diagnostics

2021 ◽  
Author(s):  
Rachael V. Dixon ◽  
Eldhose Skaria ◽  
Wing Man Lau ◽  
Philip Manning ◽  
Mark A. Birch-Machin ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Point Of Care ◽  
Disease Diagnostics ◽  
Infectious Disease Diagnostics

scholarly journals Third-Generation Sequencing in the Clinical Laboratory: Exploring the Advantages and Challenges of Nanopore Sequencing

2019 ◽  
Vol 58 (1) ◽  
Author(s):  
Lauren M. Petersen ◽  
Isabella W. Martin ◽  
Wayne E. Moschetti ◽  
Colleen M. Kershaw ◽  
Gregory J. Tsongalis
Keyword(s):  
Infectious Disease ◽  
Pathogen Detection ◽  
Clinical Laboratory ◽  
Low Cost ◽  
Turnaround Time ◽  
Metagenomic Sequencing ◽  
Nanopore Sequencing ◽  
Advantages And Disadvantages ◽  
Disease Diagnostics ◽  
Infectious Disease Diagnostics

ABSTRACT Metagenomic sequencing for infectious disease diagnostics is an important tool that holds promise for use in the clinical laboratory. Challenges for implementation so far include high cost, the length of time to results, and the need for technical and bioinformatics expertise. However, the recent technological innovation of nanopore sequencing from Oxford Nanopore Technologies (ONT) has the potential to address these challenges. ONT sequencing is an attractive platform for clinical laboratories to adopt due to its low cost, rapid turnaround time, and user-friendly bioinformatics pipelines. However, this method still faces the problem of base-calling accuracy compared to other platforms. This review highlights the general challenges of pathogen detection in clinical specimens by metagenomic sequencing, the advantages and disadvantages of the ONT platform, and how research to date supports the potential future use of nanopore sequencing in infectious disease diagnostics.

scholarly journals Quality Management for Point-Of-Care Testing of Pathogen Nucleic Acids: Chinese Expert Consensus

2021 ◽  
Vol 11 ◽  
Author(s):  
Xi Mo ◽  
Xueliang Wang ◽  
Zhaoqin Zhu ◽  
Yuetian Yu ◽  
Dong Chang ◽  
...  
Keyword(s):  
Public Health ◽  
Nucleic Acid ◽  
Quality Management ◽  
Clinical Laboratory ◽  
Point Of Care ◽  
Conventional Polymerase Chain Reaction ◽  
Laboratory Medicine ◽  
Expert Consensus ◽  
Nucleic Acid Detection ◽  
Point Of Care Testing

COVID-19 continues to circulate globally in 2021, while under the precise policy implementation of China’s public health system, the epidemic was quickly controlled, and society and the economy have recovered. During the pandemic response, nucleic acid detection of SARS-CoV-2 has played an indispensable role in the first line of defence. In the cases of emergency operations or patients presenting at fever clinics, nucleic acid detection is required to be performed and reported quickly. Therefore, nucleic acid point-of-care testing (POCT) technology for SARS-CoV-2 identification has emerged, and has been widely carried out at all levels of medical institutions. SARS-CoV-2 POCT has served as a complementary test to conventional polymerase chain reaction (PCR) batch tests, thus forming an experimental diagnosis platform that not only guarantees medical safety but also improves quality services. However, in view of the complexity of molecular diagnosis and the biosafety requirements involved, pathogen nucleic acid POCT is different from traditional blood-based physical and chemical index detection. No guidelines currently exist for POCT quality management, and there have been inconsistencies documented in practical operation. Therefore, Shanghai Society of Molecular Diagnostics, Shanghai Society of Laboratory Medicine, Clinical Microbiology Division of Shanghai Society of Microbiology and Shanghai Center for Clinical Laboratory have cooperated with experts in laboratory medicine to generate the present expert consensus. Based on the current spectrum of major infectious diseases in China, the whole-process operation management of pathogen POCT, including its application scenarios, biosafety management, personnel qualification, performance verification, quality control, and result reporting, are described here. This expert consensus will aid in promoting the rational application and robust development of this technology in public health defence and hospital infection management.

scholarly journals A Chemical-Enhanced System for CRISPR-Based Nucleic Acid Detection

2021 ◽  
Author(s):  
Zihan Li ◽  
Wenchang Zhao ◽  
Shixin Ma ◽  
Zexu Li ◽  
Yingjia Yao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Detection System ◽  
Point Of Care ◽  
Detection Sensitivity ◽  
Nucleic Acid Detection ◽  
Chemical Additives ◽  
Lateral Flow Strip ◽  
Viral Pathogens ◽  
Detection Systems ◽  
System Robustness

The CRISPR-based nucleic acid detection systems such as SHERLOCK, DETECTR and HOLMES have shown great potential for point-of-care testing of viral pathogens, especially in the context of COVID-19 pandemic. Here we optimize several key parameters of reaction chemistry and develop a Chemical Enhanced CRISPR Detection system for nucleic acid (termed CECRID). For the Cas12a/Cas13a-based signal detection phase, we determine buffer conditions and substrate range for optimal detection performance. By comparing several chemical additives, we find that addition of L-proline can secure or enhance Cas12a/Cas13a detection capability. For isothermal amplification phase with typical LAMP and RPA methods, inclusion of L-proline can also enhance specific target amplification as determined by CRISPR detection. Using SARS-CoV-2 pseudovirus, we demonstrate CECRID has enhanced detection sensitivity over chemical additive-null method with either fluorescence or lateral flow strip readout. Thus, CECRID provides an improved detection power and system robustness towards practical application of CRISPR-based diagnostics.

scholarly journals CRISPR-Assisted DNA Detection, a novel dCas9-based DNA detection technique

2020 ◽  
Author(s):  
Xinhui Xu ◽  
Tao Luo ◽  
Jinliang Gao ◽  
Na Lin ◽  
Weiwei Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Room Temperature ◽  
Dna Detection ◽  
Point Of Care ◽  
Hpv Infection ◽  
Solid Support ◽  
Human Papillomaviruses ◽  
Nucleic Acid Detection ◽  
Detection Techniques ◽  
Signal Readout

AbstractNucleic acid detection techniques are always critical to diagnosis, especially in the background of the present COVID-19 pandemic. The simple and rapid detection techniques with high sensitivity and specificity are always urgently needed. However, the current nucleic acid detection techniques are still limited the traditional amplification and hybridization. To overcome the limitation, we here develop a CRISPR/Cas9-assisted DNA detection (CADD). In this detection, DNA sample is incubated with a pair of capture sgRNAs (sgRNAa and sgRNAb) specific to a target DNA, dCas9, a signal readout-related probe, and an oligo-coated solid support beads or microplate at room temperature for 15 min. During this incubation, the dCas9-sgRNA-DNA complex is formed and captured on solid support by the capture sequence of sgRNAa and the signal readout-related probe is captured by the capture sequence of sgRNAb. Finally the detection result is reported by a fluorescent or colorimetric signal readout. This detection was verified by detecting DNA of bacteria, cancer cell and virus. Especially, by designing a set of sgRNAs specific to 15 high-risk human papillomaviruses (HPVs), the HPV infection in 64 clinical cervical samples were successfully detected by the method. All detections can be finished in 30 minutes at room temperature. This detection holds promise for rapid on-the-spot detection or point-of-care testing (POCT).

scholarly journals Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics

2018 ◽  
Vol 119 (2) ◽  
pp. 1456-1518 ◽  
Author(s):  
Christine F. Markwalter ◽  
Andrew G. Kantor ◽  
Carson P. Moore ◽  
Kelly A. Richardson ◽  
David W. Wright
Keyword(s):  
Infectious Disease ◽  
Disease Diagnostics ◽  
Infectious Disease Diagnostics ◽  
Inorganic Complexes
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