scholarly journals Ring-Fused Diphenylchlorins as Potent Photosensitizers for Photodynamic Therapy Applications: In Vitro Tumor Cell Biology and in Vivo Chick Embryo Chorioallantoic Membrane Studies

ACS Omega ◽  
2019 ◽  
Vol 4 (17) ◽  
pp. 17244-17250 ◽  
Author(s):  
Bruno F. O. Nascimento ◽  
Mafalda Laranjo ◽  
Nelson A. M. Pereira ◽  
João Dias-Ferreira ◽  
Marta Piñeiro ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Chick Embryo ◽  
Tumor Cell ◽  
Cell Biology ◽  
Chorioallantoic Membrane ◽  
Tumor Cell Biology ◽  
Chick Embryo Chorioallantoic Membrane

scholarly journals The Potential Use of Electrochemotherapy in the Treatment of Uveal Melanoma: In Vitro Results in 3D Tumor Cultures and In Vivo Results in a Chick Embryo Model

Cancers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1344 ◽  
Author(s):  
Miltiadis Fiorentzis ◽  
Arne Viestenz ◽  
Udo Siebolts ◽  
Berthold Seitz ◽  
Sarah E. Coupland ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Uveal Melanoma ◽  
Chorioallantoic Membrane ◽  
In Vivo Model ◽  
Metastatic Lesions ◽  
Diphenyltetrazolium Bromide ◽  
Chick Embryo Chorioallantoic Membrane ◽  
Tumors Of The Liver

Uveal melanoma (UM) is the most common primary intraocular tumor that arises from neoplastic melanocytes in the choroid, iris, and ciliary body. Electrochemotherapy (ECT) has been successfully established for the treatment of skin and soft tissue metastatic lesions, deep-seated tumors of the liver, bone metastases, and unresectable pancreas lesions. The aim of this study was to evaluate the effect of ECT in vitro in 3D spheroid culture systems in primary and metastatic UM cell lines. We also investigated the chick embryo chorioallantoic membrane (CAM) as an in vivo model system for the growth and treatment of UM tumors using ECT. The cytotoxic effect of ECT in 3D spheroids was analyzed seven days following treatment by assessment of the size and MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction] assay. The cytotoxicity of ECT after intratumoral or intraarterial administration was evaluated histologically. In vitro and in vivo ECT caused a significant reduction in tumor size and viability compared to electroporation or chemotherapy in both sections of our study. The current results underline the effectiveness of ECT in the treatment of UM and prepare the way for further investigation of its potential application in UM.

scholarly journals Photobiomodulation (PBM) promotes angiogenesis in-vitro and in chick embryo chorioallantoic membrane model

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Raimund Winter ◽  
Peter Dungel ◽  
Frederike Marie Josephine Reischies ◽  
Sabrina Rohringer ◽  
Paul Slezak ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Chorioallantoic Membrane ◽  
Membrane Model ◽  
Chick Embryo Chorioallantoic Membrane

The Chick Embryo Chorioallantoic Membrane as an in vivo Wound Healing Model

1996 ◽  
Vol 192 (10) ◽  
pp. 1068-1076 ◽  
Author(s):  
D. Ribatti ◽  
A. Vacca ◽  
G. Ranieri ◽  
S. Sorino ◽  
L. Roncali
Keyword(s):  
Wound Healing ◽  
Chick Embryo ◽  
Chorioallantoic Membrane ◽  
Chick Embryo Chorioallantoic Membrane ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals The Chick Embryo Chorioallantoic Membrane as a Model for in vivo Research on Anti-Angiogenesis

2000 ◽  
Vol 1 (1) ◽  
pp. 73-82 ◽  
Author(s):  
Domenico Ribatti ◽  
Angelo Vacca ◽  
Luisa Roncali ◽  
Franco Dammacco
Keyword(s):  
Chick Embryo ◽  
Chorioallantoic Membrane ◽  
Chick Embryo Chorioallantoic Membrane

uPAR – uPA – PAI-1 interactions and signaling: A vascular biologist’s view

2007 ◽  
Vol 97 (03) ◽  
pp. 336-342 ◽  
Author(s):  
Judit Mihaly ◽  
Gerald Prager ◽  
Bernd Binder
Keyword(s):  
Intracellular Signaling ◽  
Cell Biology ◽  
Large Body ◽  
Vascular Biology ◽  
General Mechanism ◽  
Tumor Cell Biology ◽  
Type Plasminogen Activator ◽  
Pai 1

SummaryThe urokinase-type plasminogen activator (uPA), its inhibitor PA I-1 and its cellular receptor (uPAR), play a pivotal role in pericellular proteolysis. In addition, through their interactions with extracellular matrix proteins as well as with transmembrane receptors and other links to the intracellular signaling machinery, they modulate cell migration, cell-matrix interactions and signaling pathways. A large body of experimental evidence from in-vitro and in-vivo data as well as from the clinics indicates an important role of the uPA-uPAR-PAI-1 systems in cancer. In addition to their role in tumor cell biology, the uPA-uPAR-PAI-1 systems are also important for vascular biology by modulating angiogenesis and by altering migration of smooth muscle cells and fibrin deposition in atherosclerosis and restenosis. This review will focus on the general mechanism of uPAR/uPA/PAI-1 interactions and signaling and the possible relevance of this system in vascular biology.

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