scholarly journals Multiplex Detection of Nucleic Acids Using Recombinase Polymerase Amplification and a Molecular Colorimetric 7-Segment Display

ACS Omega ◽  
2019 ◽  
Vol 4 (7) ◽  
pp. 11388-11396 ◽  
Author(s):  
Jia Li ◽  
Nina M. Pollak ◽  
Joanne Macdonald
Keyword(s):  
Nucleic Acids ◽  
Recombinase Polymerase Amplification ◽  
Multiplex Detection

scholarly journals Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA

Lab on a Chip ◽  
2015 ◽  
Vol 15 (13) ◽  
pp. 2759-2766 ◽  
Author(s):  
Friedrich Schuler ◽  
Frank Schwemmer ◽  
Martin Trotter ◽  
Simon Wadle ◽  
Roland Zengerle ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Absolute Quantification ◽  
Recombinase Polymerase Amplification ◽  
Dead Volume

Centrifugal step emulsification enables dead volume free emulsification, it was applied to the first digital droplet recombinase polymerase amplification (RPA).

scholarly journals Variation-Tolerant Capture and Multiplex Detection of Nucleic Acids: Application to Detection of Microbes

2012 ◽  
Vol 50 (10) ◽  
pp. 3208-3215 ◽  
Author(s):  
C. Ohrmalm ◽  
R. Eriksson ◽  
M. Jobs ◽  
M. Simonson ◽  
M. Stromme ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Multiplex Detection

scholarly journals Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153359 ◽  
Author(s):  
Zhao Li ◽  
Yong Liu ◽  
Qingquan Wei ◽  
Yuanjie Liu ◽  
Wenwen Liu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Absolute Quantification ◽  
Recombinase Polymerase Amplification

scholarly journals Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip

2011 ◽  
Vol 83 (9) ◽  
pp. 3533-3540 ◽  
Author(s):  
Feng Shen ◽  
Elena K. Davydova ◽  
Wenbin Du ◽  
Jason E. Kreutz ◽  
Olaf Piepenburg ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Recombinase Polymerase Amplification

Multiplex detection of nucleic acids using a low cost microfluidic chip and a personal glucose meter at the point-of-care

2014 ◽  
Vol 50 (29) ◽  
pp. 3824-3826 ◽  
Author(s):  
Qing Wang ◽  
Hui Wang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Fang Liu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Microfluidic Chip ◽  
Dna Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Multiplex Detection ◽  
Personal Glucose Meter ◽  
Glucose Meter

A simple assay for multiplex DNA detection has been developed using a low cost microfluidic chip and a personal glucose meter.

scholarly journals Gold Island-Enhanced Multiplex Quantum Dots Fluorescent System for Biomedical Analysis of Circulating Tumor Nucleic Acids

2021 ◽  
Author(s):  
Jingyan Lin ◽  
Yunxia Wu ◽  
Ronghua Yang ◽  
Jingru Wang ◽  
Wenjie Wu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Nucleic Acids ◽  
Point Mutations ◽  
High Sensitivity ◽  
Clinical Samples ◽  
Multiplex Detection ◽  
Biomedical Analysis ◽  
High Detection Rate ◽  
Potential Marker ◽  
Highly Sensitive

Abstract Background: Circulating tumor nucleic acids (CTNAs) have been employed as the potential marker for tumor diagnosis and management, which are highly related to the tumorigenesis, progression and metastasis processes. Therefore, it is of significance to develop a highly-sensitive and reliable methods for detection of CTNAs, especially the multiplex point mutation detection of blood-derived CTNAs. Results: Herein, a gold island-enhanced multiplex quantum dots (QDs) fluorescent platform was constructed for highly-sensitive detection of CTNAs in serum. The gold island-enhanced multiplex fluorescent strategy was designed as the highly-efficient signal giving-out mode which could amplify the fluorescence of QDs, realized a homogeneous nano-platform for the enrichment, multiplex detection and point mutations monitoring of CTNAs with the principle of base-stacking. A high sensitivity of 10 amol and desirable specificity were achieved, and the performance index for analysis of clinical CTNAs samples indicated that the gold island-enhanced multiplex QDs fluorescent strategy could realize multiplex point mutations detection of CTNAs in complex blood samples. Conclusions: Hence, this platform achieved high detection rate in clinical samples that suitably met the clinical-requirements for multiplex detection and point mutations monitoring of CTNAs, and thus has the potential to serve as the tumor liquid biopsy strategy based on CTNAs.

Electron Microscopy of Nucleic Acids

1974 ◽  
Vol 32 ◽  
pp. 302-303
Author(s):  
Norman Davidson
Keyword(s):  
Electron Microscopy ◽  
Nucleic Acids ◽  
Basic Protein ◽  
Molecular Biologist ◽  
Topological Properties ◽  
Protein Film ◽  
Polynucleotide Chain

The basic protein film technique for mounting nucleic acids for electron microscopy has proven to be a general and powerful tool for the working molecular biologist in characterizing different nucleic acids. It i s possible to measure molecular lengths of duplex and single-stranded DNAs and RNAs. In particular, it is thus possible to as certain whether or not the nucleic acids extracted from a particular source are or are not homogeneous in length. The topological properties of the polynucleotide chain (linear or circular, relaxed or supercoiled circles, interlocked circles, etc. ) can also be as certained.

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