Multiplex detection of nucleic acids using a low cost microfluidic chip and a personal glucose meter at the point-of-care

2014 ◽  
Vol 50 (29) ◽  
pp. 3824-3826 ◽  
Author(s):  
Qing Wang ◽  
Hui Wang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Fang Liu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Microfluidic Chip ◽  
Dna Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Multiplex Detection ◽  
Personal Glucose Meter ◽  
Glucose Meter

A simple assay for multiplex DNA detection has been developed using a low cost microfluidic chip and a personal glucose meter.

scholarly journals A glucose meter interface for point-of-care gene circuit-based diagnostics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Evan Amalfitano ◽  
Margot Karlikow ◽  
Masoud Norouzi ◽  
Katariina Jaenes ◽  
Seray Cicek ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Synthetic Biology ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Low Cost ◽  
Gene Circuit ◽  
Reporter Systems ◽  
Glucose Meter ◽  
Pandemic Response ◽  
Glucose Output

AbstractRecent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2.

scholarly journals A fully battery-powered inexpensive spectrophotometric system for high-sensitivity point-of-care analysis on a microfluidic chip

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3898-3903 ◽  
Author(s):  
Maowei Dou ◽  
Juan Lopez ◽  
Misael Rios ◽  
Oscar Garcia ◽  
Chuan Xiao ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Power Supplies ◽  
Low Resource Settings ◽  
Low Resource ◽  
External Power

A low-cost b̲a̲ttery-powered s̲pectrophotometric s̲ystem (BASS) was developed for high-sensitivity point-of-care analysis in low-resource settings on a microfluidic chip without relying on external power supplies.

Using a Personal Glucose Meter and Alkaline Phosphatase for Point-of-Care Quantification of Galactose-1-Phosphate Uridyltransferase in Clinical Galactosemia Diagnosis

2015 ◽  
Vol 10 (10) ◽  
pp. 2221-2227 ◽  
Author(s):  
Jingjing Zhang ◽  
Yu Xiang ◽  
Donna E. Novak ◽  
George E. Hoganson ◽  
Junjie Zhu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Point Of Care ◽  
Personal Glucose Meter ◽  
Glucose Meter

scholarly journals A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples

Lab on a Chip ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 753-763 ◽  
Author(s):  
Natalia M. Rodriguez ◽  
Winnie S. Wong ◽  
Lena Liu ◽  
Rajan Dewar ◽  
Catherine M. Klapperich
Keyword(s):  
Nucleic Acids ◽  
Point Of Care ◽  
Low Cost ◽  
Molecular Diagnostic ◽  
Clinical Samples ◽  
Fully Integrated

We present a low-cost, disposable, and fully-integrated paperfluidic molecular diagnostic chip for sample-to-result functionality at the point-of-care.

scholarly journals Multiplex Solid-Phase RPA Coupled CRISPR-Based Visual Detection of SARS-CoV-2

2021 ◽  
Author(s):  
Xiaochen Qin ◽  
Yuyuan Zhou ◽  
Ratul Paul ◽  
Yue Wu ◽  
Yaling Liu
Keyword(s):  
Solid Phase ◽  
Dna Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Control Sample ◽  
Virus Detection ◽  
Cost Effective ◽  
Analytical Sensitivity ◽  
One Pot ◽  
Hpv Dna

COVID-19 has challenged the world's public health and led to over 4.5 million deaths. A rapid, sensitive, and cost-effective point-of-care virus detection device is crucial to the control and surveillance of the contagious severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic. Here we demonstrate a solid phase isothermal recombinase polymerase amplification coupled CRISPR-based (spRPA-CRISPR) assay for on-chip multiplexed, sensitive, and visual COVID-19 DNA detection. By targeting the SARS-CoV-2 structure protein encoded genomes, two specific genes were simultaneously detected with the control sample without cross-interaction with other sequences. The endpoint signal can be directly visualized for rapid detection of COVID-19. The amplified target sequences were immobilized on the one-pot device surface and detected using the mixed Cas12a-crRNA collateral cleavage of reporter released fluorescent signal when specific genes were recognized. The system was tested with samples of a broad range of concentrations (20 to 2x105 copies) and showed analytical sensitivity down to 20 copies per reaction. Furthermore, a low-cost LED UV flashlight (~$12) was used to provide a visible SARS-CoV-2 detection signal of the spRPA-CRISPR assay which could be purchased online easily. Thus, our platform provides a sensitive and easy-to-read multiplexed gene detection method with the capacity to specifically identify low concentration genes. Similar CRISPR biosensor chips can support a broad range of applications such as HPV DNA detection, influenza SARS-CoV-2 multiplex detection, and other infectious disease testing assays.

scholarly journals Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1585
Author(s):  
Emanuele Luigi Sciuto ◽  
Antonio Alessio Leonardi ◽  
Giovanna Calabrese ◽  
Giovanna De Luca ◽  
Maria Anna Coniglio ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Analytical Methods ◽  
Point Of Care ◽  
Low Cost ◽  
Genetic Material ◽  
Treatment And Prevention ◽  
Polymerase Chain ◽  
Silicon Based ◽  
User Friendly ◽  
Diagnosis Of Infection

The analysis of viral nucleic acids (NA), DNA or RNA, is a crucial issue in the diagnosis of infections and the treatment and prevention of related human diseases. Conventional nucleic acid tests (NATs) require multistep approaches starting from the purification of the pathogen genetic material in biological samples to the end of its detection, basically performed by the consolidated polymerase chain reaction (PCR), by the use of specialized instruments and dedicated laboratories. However, since the current NATs are too constraining and time and cost consuming, the research is evolving towards more integrated, decentralized, user-friendly, and low-cost methods. These will allow the implementation of massive diagnoses addressing the growing demand of fast and accurate viral analysis facing such global alerts as the pandemic of coronavirus disease of the recent period. Silicon-based technology and microfluidics, in this sense, brought an important step up, leading to the introduction of the genetic point-of-care (PoC) systems. This review goes through the evolution of the analytical methods for the viral NA diagnosis of infection diseases, highlighting both advantages and drawbacks of the innovative emerging technologies versus the conventional approaches.

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