scholarly journals Self-Assembly of a Dentinogenic Peptide Hydrogel

ACS Omega ◽  
2018 ◽  
Vol 3 (6) ◽  
pp. 5980-5987 ◽  
Author(s):  
Peter K. Nguyen ◽  
William Gao ◽  
Saloni D. Patel ◽  
Zain Siddiqui ◽  
Saul Weiner ◽  
...  
Keyword(s):  
Self Assembly ◽  
Peptide Hydrogel

scholarly journals Nanostructure, Self-Assembly, Mechanical Properties, and Antioxidant Activity of a Lupin-Derived Peptide Hydrogel

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 294
Author(s):  
Raffaele Pugliese ◽  
Anna Arnoldi ◽  
Carmen Lammi
Keyword(s):  
Mechanical Properties ◽  
Antioxidant Activity ◽  
Self Assembly ◽  
Antioxidant Activities ◽  
Functional Materials ◽  
Cd Spectroscopy ◽  
Beneficial Effects ◽  
Naturally Occurring ◽  
New Research ◽  
Peptide Hydrogel

Naturally occurring food peptides are frequently used in the life sciences due to their beneficial effects through their impact on specific biochemical pathways. Furthermore, they are often leveraged for applications in areas as diverse as bioengineering, medicine, agriculture, and even fashion. However, progress toward understanding their self-assembling properties as functional materials are often hindered by their long aromatic and charged residue-enriched sequences encrypted in the parent protein sequence. In this study, we elucidate the nanostructure and the hierarchical self-assembly propensity of a lupin-derived peptide which belongs to the α-conglutin (11S globulin, legumin-like protein), with a straightforward N-terminal biotinylated oligoglycine tag-based methodology for controlling the nanostructures, biomechanics, and biological features. Extensive characterization was performed via Circular Dichroism (CD) spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), rheological measurements, and Atomic Force Microscopy (AFM) analyses. By using the biotin tag, we obtained a thixotropic lupin-derived peptide hydrogel (named BT13) with tunable mechanical properties (from 2 to 11 kPa), without impairing its spontaneous formation of β-sheet secondary structures. Lastly, we demonstrated that this hydrogel has antioxidant activity. Altogether, our findings address multiple challenges associated with the development of naturally occurring food peptide-based hydrogels, offering a new tool to both fine tune the mechanical properties and tailor the antioxidant activities, providing new research directions across food chemistry, biochemistry, and bioengineering.

Light-Driven Dissipative Self-Assembly of a Peptide Hydrogel

2021 ◽  
Author(s):  
Mengmeng Liu ◽  
Cassidy Creemer ◽  
Thomas Reardon ◽  
Jon Parquette
Keyword(s):  
Temporal Resolution ◽  
Self Assembly ◽  
Light Energy ◽  
Waste Production ◽  
Wavelength Tunability ◽  
Fuel Source ◽  
Peptide Hydrogel

Light energy provides an attractive fuel source for energy dissipating systems because of the lack of waste production, wavelength tunability and the potential for spatial and temporal resolution. In this...

RADA16: A Self-Assembly Peptide Hydrogel for the Application in Tissue Regeneration

2014 ◽  
Vol 4 (12) ◽  
pp. 1019-1029 ◽  
Author(s):  
Qingyuan Meng ◽  
Shenglian Yao ◽  
Xiumei Wang ◽  
Yingying Chen
Keyword(s):  
Tissue Regeneration ◽  
Self Assembly ◽  
Peptide Hydrogel

scholarly journals Hierarchical supramolecular hydrogels: self-assembly by peptides and photo-controlled release via host–guest interaction

2017 ◽  
Vol 53 (92) ◽  
pp. 12450-12453 ◽  
Author(s):  
Chih-Wei Chu ◽  
Bart Jan Ravoo
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Peptide Hydrogel ◽  
Guest Chemistry

Using photoresponsive host–guest chemistry, three different payloads can be photo-released successively from the same peptide hydrogel.

scholarly journals A Rapid Self-Assembly Peptide Hydrogel for Recruitment and Activation of Immune Cells

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 419
Author(s):  
Ruyue Luo ◽  
Yuan Wan ◽  
Xinyi Luo ◽  
Guicen Liu ◽  
Zhaoxu Li ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Dendritic Cells ◽  
Self Assembly ◽  
Immune Cell ◽  
Three Dimensional ◽  
Hydrophobic Surface ◽  
Inhibitory Effect ◽  
Blue Staining ◽  
Peptide Hydrogel

Self-assembly peptide nanotechnology has attracted much attention due to its regular and orderly structure and diverse functions. Most of the existing self-assembly peptides can form aggregates with specific structures only under specific conditions and their assembly time is relatively long. They have good biocompatibility but no immunogenicity. To optimize it, a self-assembly peptide named DRF3 was designed. It contains a hydrophilic and hydrophobic surface, using two N-terminal arginines, leucine, and two c-terminal aspartate and glutamic acid. Meanwhile, the c-terminal of the peptide was amidated, so that peptide segments were interconnected to increase diversity. Its characterization, biocompatibility, controlled release effect on antigen, immune cell recruitment ability, and antitumor properties were examined here. Congo red/aniline blue staining revealed that peptide hydrogel DRF3 could be immediately gelled in PBS. The stable β-sheet secondary structure of DRF3 was confirmed by circular dichroism spectrum and IR spectra. The observation results of cryo-scanning electron microscopy, transmission electron microscopy, and atomic force microscopy demonstrated that DRF3 formed nanotubule-like and vesicular structures in PBS, and these structures interlaced with each other to form ordered three-dimensional nanofiber structures. Meanwhile, DRF3 showed excellent biocompatibility, could sustainably and slowly release antigens, recruit dendritic cells and promote the maturation of dendritic cells (DCs) in vitro. In addition, DRF3 has a strong inhibitory effect on clear renal cell carcinoma (786-0). These results provide a reliable basis for the application of peptide hydrogels in biomedical and preclinical trials.

Self-Assembly of an Antiangiogenic Nanofibrous Peptide Hydrogel

2018 ◽  
Vol 1 (3) ◽  
pp. 865-870 ◽  
Author(s):  
Peter K. Nguyen ◽  
Biplab Sarkar ◽  
Zain Siddiqui ◽  
Michael McGowan ◽  
Patricia Iglesias-Montoro ◽  
...  
Keyword(s):  
Self Assembly ◽  
Peptide Hydrogel

scholarly journals Cross-Linked Self-Assembling Peptides and Their Post-Assembly Functionalization via One-Pot and In Situ Gelation System

2020 ◽  
Vol 21 (12) ◽  
pp. 4261
Author(s):  
Raffaele Pugliese ◽  
Fabrizio Gelain
Keyword(s):  
Mechanical Properties ◽  
Self Assembly ◽  
Self Healing ◽  
One Pot ◽  
Self Assembling ◽  
In Situ Gelation ◽  
Wide Range ◽  
Peptide Hydrogels ◽  
Peptide Hydrogel

Supramolecular nanostructures formed through peptide self-assembly can have a wide range of applications in the biomedical landscape. However, they often lose biomechanical properties at low mechanical stress due to the non-covalent interactions working in the self-assembling process. Herein, we report the design of cross-linked self-assembling peptide hydrogels using a one-pot in situ gelation system, based on 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide/N-hydroxysulfosuccinimide (EDC/sulfo–NHS) coupling, to tune its biomechanics. EDC/sulfo–NHS coupling led to limited changes in storage modulus (from 0.9 to 2 kPa), but it significantly increased both the strain (from 6% to 60%) and failure stress (from 19 to 35 Pa) of peptide hydrogel without impairing the spontaneous formation of β-sheet-containing nano-filaments. Furthermore, EDC/sulfo–NHS cross-linking bestowed self-healing and thixotropic properties to the peptide hydrogel. Lastly, we demonstrated that this strategy can be used to incorporate bioactive functional motifs after self-assembly on pre-formed nanostructures by functionalizing an Ac-LDLKLDLKLDLK-CONH2 (LDLK12) self-assembling peptide with the phage display-derived KLPGWSG peptide involved in the modulation of neural stem cell proliferation and differentiation. The incorporation of a functional motif did not alter the peptide’s secondary structure and its mechanical properties. The work reported here offers new tools to both fine tune the mechanical properties of and tailor the biomimetic properties of self-assembling peptide hydrogels while retaining their nanostructures, which is useful for tissue engineering and regenerative medicine applications.

Functionalized self-assembly polypeptide hydrogel scaffold applied in modulation of neural progenitor cell behavior

2016 ◽  
Vol 32 (1) ◽  
pp. 45-60 ◽  
Author(s):  
Mingyong Gao ◽  
Haiyin Tao ◽  
Tao Wang ◽  
Ailin Wei ◽  
Bin He
Keyword(s):  
Extracellular Matrix ◽  
Progenitor Cells ◽  
Self Assembly ◽  
Progenitor Cell ◽  
Neural Progenitor Cells ◽  
Three Dimensional ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Hydrogel Scaffold ◽  
Peptide Hydrogel

Three-dimensional cell culturing provides an appealing biomimetic platform to probe the biological effects of a designed extracellular matrix on the behavior of seeded neural stem or neural progenitor cells. This culturing model serves as an important tool to investigate functional regulators involved in proliferation and differentiation of neural progenitor cells. This study aims to reconstruct a polypeptide hydrogel matrix functionally integrated with cyclo-RGD motif [c(RGDfK)] for initial exploration of neural progenitor cell behavior in three-dimensional culture. Three types of hydrogel scaffolds including Type I collagen, RADA16 self-assembly peptide, and RADA16-c(RGDfK) self-assembly peptide hydrogel were employed to serve as the culturing extracellular matrix of neonatal rat spinal neural progenitor cells. The neural adhesion of functionalized self-assembly peptide hydrogel was acquired prior to its RADA16 counterpart with neural progenitor cell seeding tests. The biophysiological properties of self-assembly peptide hydrogel scaffolds were then detected by scanning electron microscopy and rheology measurements. The biological behavior of embedded neural progenitor cells including cell proliferation and differentiation in three-dimensional niche were analyzed by MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] tests and immunocytochemistry fluorescence staining. The 1% (w/v) RADA16-c(RGDfK) hydrogel scaffold [R16-c(RGDfK)HS] demonstrated an elastic modulus(312 ± 5.7 Pa) compatible with central neural cells, which significantly facilitated the proliferation of embedded neural progenitor cells. Compared to collagen hydrogel, both RADA16 and RADA16-c(RGDfK) hydrogel scaffold improved the cellular proliferation and neuronal differentiation of neural progenitor cells in a three-dimensional culture model. In order to model neuronal regeneration, introduction of neurotrophin-3 in the differentiation environment significantly increased the neuronal differentiation in which the ratio of Tuj-1-positive cell number increased to 72.5% ± 4.7% in the c(RGDfK)-functionalized three-dimensional matrix environment at 7 days in culture. Collectively, the present R16-c(RGDfK)HS displays excellent central neural biocompatibility and emerges as a promising bioengineered extracellular matrix niche of neural stem or progenitor cells, building a solid foundation for the subsequent in vitro and in vivo studies including neural repair, regeneration, and development.

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