scholarly journals Site-Selective RNA Activation by Acridine-Modified Oligodeoxynucleotides in Metal-Ion Catalyzed Hydrolysis: A Comprehensive Study

ACS Omega ◽  
2017 ◽  
Vol 2 (9) ◽  
pp. 5370-5377 ◽  
Author(s):  
Akinori Kuzuya ◽  
Kenzo Machida ◽  
Yun Shi ◽  
Keita Tanaka ◽  
Makoto Komiyama
Keyword(s):  
Metal Ion ◽  
Rna Activation ◽  
Site Selective ◽  
Comprehensive Study

Efficient Site-selective RNA Activation and Scission Achieved by Geometry Control of Acridine Intercalation in RNA/DNA Heteroduplex

2009 ◽  
Vol 38 (5) ◽  
pp. 432-433 ◽  
Author(s):  
Akinori Kuzuya ◽  
Yun Shi ◽  
Keita Tanaka ◽  
Kenzo Machida ◽  
Makoto Komiyama
Keyword(s):  
Rna Activation ◽  
Site Selective

Ca2+ metal ion adducts with cytosine, cytidine and cytidine 5′-monophosphate: a comprehensive study of calcium reactivity towards building units of nucleic acids

CrystEngComm ◽  
2014 ◽  
Vol 16 (35) ◽  
pp. 8286-8296 ◽  
Author(s):  
Nadia Marino ◽  
Donatella Armentano ◽  
Claudia Zanchini ◽  
Giovanni De Munno
Keyword(s):  
Nucleic Acids ◽  
Binding Sites ◽  
Calcium Ion ◽  
Metal Ion ◽  
Comprehensive Study

Six new Ca(ii) adducts with cytosine (cyt), cytidine (H2cyd) and cytidine 5′-monophosphate (CMP) are presented. H2cyd and CMP show unprecedented binding sites for the calcium ion.

Metal-Catalyzed Site-Selective Monoacylation of Diols in Aqueous Media

Synthesis ◽  
2019 ◽  
Vol 51 (20) ◽  
pp. 3784-3791
Author(s):  
Yuyang Li ◽  
Ronald Kluger
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Aqueous Media ◽  
Water Soluble ◽  
Solvent Mixtures ◽  
Phosphate Monoesters ◽  
Stable Reagent ◽  
Metal Catalyzed ◽  
Site Selective ◽  
Acyl Phosphate

Site-selective reactions of water-soluble biomolecules are being developed to produce efficient conversions in water and water­/solvent mixtures. This review focuses on the use of designs based on bis-bidentate chelation of large metal ions by diols to be acylated by a co-chelated water-stable reagent. Topics discussed include: 1. The preparation and properties of water-stable acyl phosphate monoesters and their reactions with diol-chelated metal ions. 2. Site-selective monoaminoacylation of 3′-terminal diols of RNA and their applications in protein engineering. 3. Site-selective monoacylation of sugars with acyl phosphate monoesters associated with metal ions, including lanthanum and lead. The combination of metal ion, 1,2-diol, and acyl phosphate monoester produces site-selective reactions in aqueous media­ that can produce a general approach to site-selective mono-(amino)acylation in RNA and carbohydrates.1 Introduction2 Synthetic Aminoacylation of tRNA3 Activated Amino Acids in Water4 Metal Ions and Their Effects on the Reactivity of Acyl Phosphate Monoesters5 The Challenge of Site-Selective Acylation of Carbohydrates in Water6 Conclusions and Prospects

scholarly journals Site-Selective Artificial Ribonucleases: Renaissance of Oligonucleotide Conjugates for Irreversible Cleavage of RNA Sequences

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1732
Author(s):  
Yaroslav Staroseletz ◽  
Svetlana Gaponova ◽  
Olga Patutina ◽  
Elena Bichenkova ◽  
Bahareh Amirloo ◽  
...  
Keyword(s):  
Metal Ion ◽  
Protein Complexes ◽  
Artificial Ribonucleases ◽  
Rna Sequences ◽  
Molecular Scaffold ◽  
Catalytically Active ◽  
Recognition Motifs ◽  
The One ◽  
Site Selective

RNA-targeting therapeutics require highly efficient sequence-specific devices capable of RNA irreversible degradation in vivo. The most developed methods of sequence-specific RNA cleavage, such as siRNA or antisense oligonucleotides (ASO), are currently based on recruitment of either intracellular multi-protein complexes or enzymes, leaving alternative approaches (e.g., ribozymes and DNAzymes) far behind. Recently, site-selective artificial ribonucleases combining the oligonucleotide recognition motifs (or their structural analogues) and catalytically active groups in a single molecular scaffold have been proven to be a great competitor to siRNA and ASO. Using the most efficient catalytic groups, utilising both metal ion-dependent (Cu(II)-2,9-dimethylphenanthroline) and metal ion-free (Tris(2-aminobenzimidazole)) on the one hand and PNA as an RNA recognising oligonucleotide on the other, allowed site-selective artificial RNases to be created with half-lives of 0.5–1 h. Artificial RNases based on the catalytic peptide [(ArgLeu)2Gly]2 were able to take progress a step further by demonstrating an ability to cleave miRNA-21 in tumour cells and provide a significant reduction of tumour growth in mice.

15N and 199Hg magnetic resonance spectral investigation of inosine protonation and diamagnetic metal ion complexation

1983 ◽  
Vol 61 (11) ◽  
pp. 2445-2448 ◽  
Author(s):  
G. W. Buchanan ◽  
M. J. Bell
Keyword(s):  
Magnetic Resonance ◽  
Chemical Shift ◽  
Metal Ion ◽  
Spectral Investigation ◽  
Metal Ion Complexation ◽  
Ion Complexation ◽  
Site Selective

On the basis of complexation induced 15N chemical shift changes, the nucleoside inosine is shown to protonate preferentially at the N-7 site. This nitrogen is also favoured by Zn+2 and Hg+2 ions. 199Hg shifts support the notion that the binding of the first equivalent of Hg+2 is site selective.

Diamagnetic metal ion – nucleoside interactions in solution as studied by 15N nuclear magnetic resonance

1982 ◽  
Vol 60 (6) ◽  
pp. 787-791 ◽  
Author(s):  
G. W. Buchanan ◽  
J. B. Stothers
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Metal Ion ◽  
Chemical Shifts ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Physical Measurements ◽  
Highly Sensitive ◽  
Site Selective ◽  
Nuclear Magnetic

15N nuclear magnetic resonance chemical shifts are shown to be highly sensitive to site-selective diamagnetic metal ion binding for the nucleosides adenosine, cytidine, and guanosine. Conclusions regarding preferred complexation sites are compared with results derived from other physical measurements, and with expectations based on electron density calculations.

scholarly journals Site-selective artificial ribonuclease using pinpoint RNA activation

2001 ◽  
Vol 1 (1) ◽  
pp. 131-132 ◽  
Author(s):  
A. Kuzuya ◽  
R. Mizoguchi ◽  
M. Komiyama
Keyword(s):  
Rna Activation ◽  
Site Selective

Cooperation of metal-ion fixation and target-site activation for efficient site-selective RNA scission

2005 ◽  
Vol 10 (3) ◽  
pp. 270-274 ◽  
Author(s):  
Akinori Kuzuya ◽  
Yun Shi ◽  
Takuro Sasayama ◽  
Makoto Komiyama
Keyword(s):  
Metal Ion ◽  
Target Site ◽  
Site Selective
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